Comparison of two different methods for evaluating ...
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Comparison of two different methods for evaluating boar semen morphology Comparación de dos diferentes metodologías de evaluación de la morfología del semen porcino G Oberlender*, LDS Murgas, MG Zangeronimo, AC Silva, LJ Pereira, RAL Muzzi Department of Veterinary Medicine, Division of Physiology and Pharmacology, Federal University of Lavras, Lavras, Minas Gerais, Brazil.
RESUMEN El objetivo de este estudio fue comparar el uso y la eficacia de dos técnicas para evaluación de la morfología espermática del semen porcino. La preparación húmeda con una solución de formaldehído-citrato 2,94% y otra con el uso del colorante Rosa de Bengala. Se evaluaron 58 eyaculados de ocho verracos de alto desempeño, con edad entre 20 y 25 meses. Después de la recogida del semen, la morfología espermática fue evaluada por dos técnicas: la preparación húmeda convencional (solución de formaldehído-citrato 2,94%) observada en microscopio de contraste de fase (1.000X de magnificación) y la técnica que utiliza un frotis preparado a partir del colorante Rosa de Bengala, analizado bajo un microscopio óptico (400X de magnificación). Se observó que el porcentaje de cambios de la cabeza, acrosoma, pieza intermedia y el total de cambios morfológicos no fueron diferentes (P > 0,05) entre los dos métodos. Se obtuvo un mayor número de defectos de la cola (P
