Conc (nM) Amplicon length (bp) Supplementary Table 1 ... - PLOS

Gene ID PLUGIN AGAP009098 AGAP009099 AGAP009100 AEDES 1 AEDES 2 CULEX 1 CULEX 2 RpS7 Y-specific DsRed

Sequence (5’-3’) TGATTCAACCGTAGACATGAAGG CCACCATACAACGGAACGAC CACCATTCCGATTGAGTTTGTT CAGTTGAGTGCTTCCCTTGA CAACCATGTCTACCACCTCTACC ATCCTTTATCGCACTGTTCGTC AGAGTTATTCGCTTCCGCAG GGTGTGCTTTCTCTTCCATGT CAGTGGATTTTTACCTACTGTTCAATC TCGCCAAATCATTGTCGTATC TCGTTTTATTTGCTGTTCAATCC GAATTTCCACACCGAAGGTC TACTTGCTGTTTAATCCGTGGTG TCCAGATCAAAGTGGTATCGTTC TTGTCCCCGTTGATGATGT GTACGATGAGCCTCTCCATTCT ATGGTTTTCGGATCAAAGGTG CGGCTTCAGGTCCGAGTT GGATCTGGCCAAGAGGAGTA CCCAACCAAGGTACTCTAACG ATGGTGCGCTCCTCCAAGAACG ACCTTCAGCTTCACGGTGTTGTGG

Conc (nM) 300 300 300 900 300 300 300 900 300 900 300 900 300 300 300 300 300 300 300 300 300 300

Amplicon length (bp) 87 88 150 87 113 147 100 116 111 148 146

Supplementary Table 1. Primers used for quantitative PCR. Primers against Plugin and TGases were used for qRT-PCR. Ribosomal genes were used as controls for normalization. For An. gambiae, we used against RpL19 (AGAP004422). For A. aegypti and C. quinquefasciatus, we used a single set of primers against RpS7 (AAEL009496 and CPIJ006763, respectively) which perfectly matched the sequences in both species. Primers against the Y-chromosome of An. gambiae and the dsRed-containing transgenic cassette were used to quantify relative sperm numbers by standard quantitative PCR in the remating experiment.

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