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Scott Radcliffe. Romer Labs. 130 Sandy Dr, Newark, DE 19713 scott.radcliffe@romerlabs.com www.romerlabs.com. Scott W. Radcliffe and Lukas Frank.

Scott W. Radcliffe and Lukas Frank

Romer Labs, Inc., 130 Sandy Dr, Newark, DE 19713

Objective • Determine whether a selection of popular spices may cross-react with AgraStrip Allergen assays

Results

Conclusions

Table 1. AgraStrip Allergen cross-reactivity and interference test results for various spice matrices. AgraStrip® Assay

• Verify that selected spices will not interfere with detection of a low level allergen spike

Spike

Unspiked Almond

Methods • Test cumin, turmeric, curry powder, paprika and chili powder using AgraStrip Almond, Cashew/Pistachio, Egg, Gluten G12, Peanut, Soy and Total Milk

5 ppm Almond

Unspiked Cashew/Pistachio 10 ppm Pistachio

• Verify pH of spice extracts in 6.0 – 8.0 range prior to assay, and adjust if necessary • Spike spices with almond, pistachio, egg, wheat, peanut, soy or milk at levels close to AgraStrip LOD and retest Correspondence should be addressed to: Scott Radcliffe Romer Labs 130 Sandy Dr, Newark, DE 19713 [email protected] www.romerlabs.com

Unspiked Egg 5 ppm Egg

Unspiked Gluten G12 10 ppm Gluten

Spice

Result

Cumin Turmeric Curry Powder Paprika* Chili Powder* Cumin + Turmeric + Curry Powder + Paprika* + Chili Powder* + Cumin Turmeric Curry Powder Paprika* Chili Powder* Cumin + Turmeric + Curry Powder + Paprika* + Chili Powder* + Cumin Turmeric Curry Powder Paprika* Chili Powder* Cumin + Turmeric + Curry Powder + weak line Paprika* Chili Powder* + Cumin Turmeric Curry Powder Paprika* Chili Powder* Cumin + Turmeric + Curry Powder + Paprika* + Chili Powder* +

AgraStrip® Assay

Peanut

Soy

Total Milk

Spike

Spice

Result

Cumin Turmeric Unspiked Curry Powder Paprika* Chili Powder* Cumin + Turmeric + 5 ppm Curry Powder + Peanut weak line Paprika* Chili Powder* + Cumin Turmeric Unspiked Curry Powder Paprika* Chili Powder* Cumin + Turmeric + 5 ppm Curry Powder + Soy Paprika* + Chili Powder* + Cumin Turmeric Unspiked Curry Powder Paprika Chili Powder Cumin + Turmeric + 5 ppm Curry Powder + Milk weak line Paprika* Chili Powder* +

*Extracts from paprika and chili powder were found to have pH