Conformational Plasticity of the Immunoglobulin Fc ... - Cell Press

2 downloads 0 Views 3MB Size Report
May 3, 2018 - Soumya G. Remesh,1 Anthony A. Armstrong,2 Andrew D. Mahan,2 Jinquan Luo,2,* and Michal Hammel1,3,*. 1Molecular Biophysics and ...
Short Article

Conformational Plasticity of the Immunoglobulin Fc Domain in Solution Graphical Abstract

Authors Soumya G. Remesh, Anthony A. Armstrong, Andrew D. Mahan, Jinquan Luo, Michal Hammel

Correspondence [email protected] (J.L.), [email protected] (M.H.)

In Brief Fragment crystallizable (Fc) region of immunoglobulin G controls antibody effector function through specific Fc receptors interaction. Remesh et al. developed and applied an all-atom solution scattering-based modeling tool to reveal biologically relevant conformational plasticity of the Fc isotypes. This inexpensive SAXS-based approach can also aid typical antibody engineering efforts.

Highlights d

Experimental visualization of Fc flexibility in solution

d

Flexibility variation observed between Fc isotypes in solution

d

All-atom modeling provides an accurate conformational sampling of glycoproteins

d

SAXS utilized to model