Marmoset New World Primates. Suzette Tardif1, Jean ... infection, congenital ZIKV syndrome, and demise in 100% of twin gestations. Considered with our in ...
Poster Session V
ajog.org assumption of the cost of vaccine of $37.55, vaccination was cost effective at $19,659.20 per QALY. The results of the model remain cost-effective in sensitivity analyses that vary baseline prevalence of pertussis in infants to 1%, vaccine effectiveness down to 20%, and cost of the vaccine up to $100.00. CONCLUSION: OB providers should ensure that pregnant women receive the Tdap vaccine during pregnancy, especially given recent data that show postpartum maternal vaccination and cocooning strategies are not effective. Public health strategies to encourage greater uptake of the Tdap vaccination should be employed.
& 83 (dam 1, human equivalent 14 weeks) or 68 & 72 days (dam 2, 9 weeks; Fig.1A). Serial sonograms assessed ongoing viability or demise of twin gestations, and dams were monitored daily for illness. Following delivery, placental & fetal tissues were subjected to qRTPCR for ZIKV quantification, gross fetal pathology & IHC (4G2 mAb). RESULTS: Neither dam demonstrated any evidence of illness post inoculation, and gained weight as anticipated (0.4-1.5 g/day). Viable and continuing pregnancies were confirmed by US on days 78-93, until spontaneous demise at 16-18 days post infection (Fig.1A; 21 & 17 week human equivalent dams 1 & 2, respectively). Fetal expulsion occurred within 36 hours of demise, and fetuses were friable with BPD 50% of expected (Fig.1B). Viral replication in placenta, cord, and anticipated fetal tissues recapitulated human disease (Fig.1A); these findings were confirmed by IHC of placenta & neural tissue (Fig.1C). CONCLUSION: We have demonstrated that marmosets are unique and valuable primate model for studying congenital viral infections in vivo, and ZIKV in particular. We have observed that placental infection was both necessary and sufficient for dizygotic fetal infection, congenital ZIKV syndrome, and demise in 100% of twin gestations. Considered with our in vitro data, these findings suggest that the placental trophoblast is the reservoir and portal for fetal ZIKV infection.
868 Congenital Zika Virus (ZIKV) Infection in Marmoset New World Primates Suzette Tardif1, Jean Patterson2, Manasi Tamhankar2, Maxim Seferovic3, Eumenia Castro3, Claudia Sanchez San Martin4, Melissa Suter3, Kjersti Aagaard3, Julienne Rutherford5, Charles Chiu4 1
Southwest National Primate Research Center, San Antonio, TX, 2Texas Biomedical Research Center, San Antonio, TX, 3Baylor College of Medicine, Houston, TX, 4University of California at San Francisco, San Francisco, CA, 5 University of Illinois at Chicago, Chicago, IL
OBJECTIVE: We and others have recently shown that placental tro-
phoblasts serve as a reservoir for ZIKV replication & a portal for fetal infection. Marmosets are the only primates who have dizygotic twins or triplets in each pregnancy, and have evolved a series of unique adaptations accordingly. One such unique adaptation is the significant period spent in placental development and trophoblast differentiation. In fact, nearly 40% of each marmoset dizygotic gestation is dedicated to placental development with simultaneous halting of the twin embryos implantation and development (term gestation 144 days, CRL measureable only after day 65). We reasoned that the marmoset would thus serve as a unique and relevant model to test the premise that placental infection is necessary for congenital ZIKV infection and malformations or death. STUDY DESIGN: Ultrasound dated (+/- 3d) pregnant marmoset dams (n¼2) were infected intramuscularly with 2 inoculums (.25 ml of 1x105 cfu/ml) of current pandemic strain ZIKV at gestational days 79
869 Zika virus can infect term human placentas devoid of the maternal inflammatory response Diana Villazana-Kretzer1, Stacey Schmiedecke1, Peter G. Napolitano1, Jennifer Stencel-Baerenwald1, Michael Gale2, Nicholas Ieronimakis1 1 Madigan Army Medical Center, Tacoma, WA, 2University of Washington, Settle, WA
Supplement to JANUARY 2018 American Journal of Obstetrics & Gynecology
S517
