Corrosion. Corrosion is the gradual destruction of material by chemical reaction
with its environment. – Environmental factors. • e.g. pH, salts→ linked to ...
Corrosion protection by biofilms Nardy Kip Hans van Veen NIOO-KNAW Wageningen
BioCoPro
Corrosion Corrosion is the gradual destruction of material by chemical reaction with its environment. – Environmental factors • e.g. pH, salts linked to biological processes
Corrosion • Direct costs 3% of world’s GDP • Corrosion protection – Paint, coatings – Expensive – Continuous maintenance – Hazardous for environment – Not accessible in all places
Corrosion MIC: microbiologically influenced corrosion Change of electrochemical conditions at the metal-solution interface – Acid production – Hydrogen consumption
Microbial metal corrosion – Iron oxidizers – Iron reducers – Sulfate reducers – Manganese oxidizers
Goal • BioGeoCivil (STW): – application, sustainable engineering – Understand corrosion and corrosion inhibition – the solution from the in situ situation
• How to find MICI – Using the in situ situation: • Corroded vs non-corroded spots
– Not in situ: grow biofilms on metal in lab
MICI MICI= Microbial Influenced Corrosion Inhibition – Field samples – Renovation of harbors and sheet piles in NL – Focus on carbon steel in soil
Ideal experimental set up Samples of same material from corroding vs non corroding environments
Characterization of the in situ situation
Microscopy and FISH
DNA, RNA, protein isolation
Cultivation and isolation
What can be done in that ecosystem? How can we isolate the microorganisms?
Community characterization
Community characterization
Pure cultures/biofilm
Who is there?
Who is there? What do they do?
What do they do? Corrosion protection experiments
Integration of results to test application and control
Experiments • Gouderak – Sampling of carbon steel sheet piles – Below ground analysis – Layers of mineral precipitation Total lenght: approx. 5m. Description : 1 m red corrosion deposit Sheetpile:
Other samples: Soil samples same height as deposit: Soil 1 Soil 2
1 m black powder deposit 2 m black grey deposit
Microbial communities on sheet pile compared to eachother and to soil microbial communities
workflow • 16S rRNA gene analysis to determine community composition • Tagged PCR for 454 amplicon pyrosequencing – Unidirectional 515F-806R – Samples in triplo (different tags)
Experiments PCR A1.1
PCR A1.2
Corrosion sample A
A1 tag1
PCR A1.3
A2 tag 2
PCR A1.4
A3 tag 3
PCR A1.5
Purified A1 tag1
workflow Amplicon reads
Phylogenetic analysis
QPCR
FISH
Future NGS experiments
Relate data to biochemical data So far only none corrosion
‘hints’ for isolation
Future • Analysis microbial communities Gouderak • Harbor Rotterdam – Sheet pile sampling – Microbial community analysis • In cooperation with Bioclear
– Lab experiments with sheet pile material – Microscopy – Isolations – In situ experiments
Final Microbiology
Geo-Engineering
who is present
Corrosion rates
what do they do how do they grow
Biofilm growth rates
Hydrology Measurements on porous medium Microbial and geophysical data integration Pore network model
Final Microbiology
Geo-Engineering
who is present
Corrosion rates
what do they do how do they grow
Biofilm growth rates
Control and application
Hydrology Measurements on porous medium Microbial and geophysical data integration Pore network model
