Corrosion protection by biofilms - NBIC

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Corrosion. Corrosion is the gradual destruction of material by chemical reaction with its environment. – Environmental factors. • e.g. pH, salts→ linked to ...
Corrosion protection by biofilms Nardy Kip Hans van Veen NIOO-KNAW Wageningen

BioCoPro

Corrosion Corrosion is the gradual destruction of material by chemical reaction with its environment. – Environmental factors • e.g. pH, salts linked to biological processes

Corrosion • Direct costs 3% of world’s GDP • Corrosion protection – Paint, coatings – Expensive – Continuous maintenance – Hazardous for environment – Not accessible in all places

Corrosion MIC: microbiologically influenced corrosion Change of electrochemical conditions at the metal-solution interface – Acid production – Hydrogen consumption

Microbial metal corrosion – Iron oxidizers – Iron reducers – Sulfate reducers – Manganese oxidizers

http://www.biofilmbook.com

MICI MICI= Microbial Influenced Corrosion Inhibition – Removal corrosive agents (e.g. oxygen) – Growth inhibition corrosion causing bacteria • Anti microbial

– Generation of protective layer • Biofilm growth

All experiments are performed in the lab

Goal • BioGeoCivil (STW): – application, sustainable engineering – Understand corrosion and corrosion inhibition – the solution from the in situ situation

• How to find MICI – Using the in situ situation: • Corroded vs non-corroded spots

– Not in situ: grow biofilms on metal in lab

MICI MICI= Microbial Influenced Corrosion Inhibition – Field samples – Renovation of harbors and sheet piles in NL – Focus on carbon steel in soil

Ideal experimental set up Samples of same material from corroding vs non corroding environments

Characterization of the in situ situation

Microscopy and FISH

DNA, RNA, protein isolation

Cultivation and isolation

What can be done in that ecosystem? How can we isolate the microorganisms?

Community characterization

Community characterization

Pure cultures/biofilm

Who is there?

Who is there? What do they do?

What do they do? Corrosion protection experiments

Integration of results to test application and control

Experiments • Gouderak – Sampling of carbon steel sheet piles – Below ground analysis – Layers of mineral precipitation Total lenght: approx. 5m. Description : 1 m red corrosion deposit Sheetpile:

Other samples: Soil samples same height as deposit: Soil 1 Soil 2

1 m black powder deposit 2 m black grey deposit

Microbial communities on sheet pile compared to eachother and to soil microbial communities

workflow • 16S rRNA gene analysis to determine community composition • Tagged PCR for 454 amplicon pyrosequencing – Unidirectional 515F-806R – Samples in triplo (different tags)

Experiments PCR A1.1

PCR A1.2

Corrosion sample A

A1 tag1

PCR A1.3

A2 tag 2

PCR A1.4

A3 tag 3

PCR A1.5

Purified A1 tag1

workflow Amplicon reads

Phylogenetic analysis

QPCR

FISH

Future NGS experiments

Relate data to biochemical data So far only none corrosion

‘hints’ for isolation

Future • Analysis microbial communities Gouderak • Harbor Rotterdam – Sheet pile sampling – Microbial community analysis • In cooperation with Bioclear

– Lab experiments with sheet pile material – Microscopy – Isolations – In situ experiments

Final Microbiology

Geo-Engineering

who is present

Corrosion rates

what do they do how do they grow

Biofilm growth rates

Hydrology Measurements on porous medium Microbial and geophysical data integration Pore network model

Final Microbiology

Geo-Engineering

who is present

Corrosion rates

what do they do how do they grow

Biofilm growth rates

Control and application

Hydrology Measurements on porous medium Microbial and geophysical data integration Pore network model