Binding (CPM)/Device. Omega PES. Membrane. 3000. A 500 µL sample of a 32P-labeled PCR product (400 bp, 50 ng/mL) was ce
Designed to Maximize Sample Prep Efficiency Nanosep® Centrifugal Devices Maximum Recovery Proprietary membrane chemistry Faster Processing Unique membrane design for faster flow Reproducible Manufactured for quality
Buy 2 Packs of Nanosep Centrifugal Devices,
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Buy 2 packs of Nanosep Centrifugal Devices, and get 1 pack of the same FREE! Applications
Ordering Information
• Concentrate, purify and desalt DNA, RNA and proteins
Qualifying Nanosep Centrifugal Devices
• Clean up labeling and PCR reactions
Size
• Isolate Oligos, DNA and RNA from gel slices
3k OD003C34 29301-782 100/pkg
Pall Part No.
VWR Cat. No.
Packaging
10k
OD010C34
29300-620
100/pkg
Benefits
30k
OD030C34
29300-622
100/pkg
• Typical recoveries are >90%
100k OD100C34 29300-624 100/pkg
• Simple reliable concentration and desalting of 50 µL – 500 µL samples
300k
OC300C34
29300-626
100/pkg
Visit vwr.com for additional product information.
• Available in low protein binding Omega™, Bio-Inert® and GHP membranes
Purchase your Nanosep Centrifugal Devices by visiting vwr.com, calling 1.800.932.5000, or contacting your VWR Sales Rep by December 31, 2017.
DNA Binding Nanosep Results
Redeem for your FREE pack of Nanosep Centrifugal Devices by visiting vwr.com/promotions and searching for promo code 4652. Fill in the online form before January 31, 2018.
Relative DNA Binding (CPM)/Device
6000 5000 4000 3000 2000 1000 0
Regenerated Polysulfone Omega PES Cellulose Membrane Membrane Membrane
A 500 µL sample of a 32P-labeled PCR product (400 bp, 50 ng/mL) was centrifuged at 5,000 x g in a 100K Nanosep or competitive device. The retained DNA was recovered in 40 µL TE (10 mM Tris, 1 mM EDTA, pH 8). The remaining radioactivity in the device was counted in a scintillation counter. A value at 1000 CPM roughly corresponds to 1% of the total radioactive sample. Omega membrane has the lowest non-specific binding, resulting in highest DNA recoveries. In general, DNA molecular weights do not correspond well with the MWCO because DNA is a long linear molecule. Effective retention of DNA by an ultrafiltration membrane requires a reduction in g-force to 5,000 x g. Otherwise, DNA can be forced through many MWCO membranes regardless of size.
0417 Lit. No. 93686REV Promo Code 4652 © 2017 Pall Corporation. Pall, , Bio-Inert, Nanosep, and Omega are trademark of Pall Corporation.® indicates a trademarks registered in the USA. Limit one per customer. Offer not valid where prohibited by law or institutional policy. Valid only in both US and CA. Allow up to 8 weeks for delivery of item. Offer expires December 31, 2017. 4/17, 16.1006
