Acta Chim. Slov. 2006, 53, 517–520 Technical Paper
Determination of Fipronil Residue in Soil and Water in the Rice Fields in North of Iran by RP-HPLC Method Mohammad Reza Hadjmohammadi*, Seyed Mohammad Nikou, Kamyar Kamel Department of Chemistry, University of Mazandaran, P.O.Box 453,Babolsar, Iran E-mail:
[email protected]
Received 12-06-2006
Abstract A method for the determination of amount of residue of insecticide fipronil in paddy water and soil was developed based on RP-HPLC. Trials were carried out on rice fields in Iran using a fipronil granular formulation at a rate of 40 g ai/ha just after transplanting, which corresponded to 20 kg/ha of formulated product. Liquid-Liquid Extraction (LLE) and Soxhlet extraction were applied for extraction of fipronil from paddy water and soil respectively. In paddy water, Fipronil levels declined from 0.018 to 0.0028 µg/ml from one day to 40 days after application. No analyte was observed after 40 days at levels exceeding limit of detection. Soil analysis also showed that the amount of fipronil declined from 0.032 to
