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Development and Validation of a Two-Site ... - Semantic Scholar
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CLIN. CHEM. 35/6, 953-957
(1989)
Development and Validation of a Two-Site Natriuretic Factor n Unextracted PIama Helen M.
Wendy A. Retcllffe,2
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Immunoradiometric
monoclonal antibody is radioiodinated, and the igG fraction of the antiserum is coated onto wells of a microtiter plate. Plasma or standard hANF99-1 26 (150 4) is Incubated with the radioligand in coated wells for 24 h. The detection limit is pg per well, corresponding to 2 pmol/L, with a working range (CV
