Development and Validation of RP-HPLC Method for Simultaneous
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Open Access
Austin Journal of Analytical and Pharmaceutical Chemistry Research Article
Development and Validation of RP-HPLC Method for Simultaneous Estimation of Levocloperastine Fendizoate and Chlorpheniramine Maleate in their Combined Dosage Form Sharma B1, Satone D2, Pradhan P1, Jain H1 and Meshram D1* 1 Department of Quality Assurance, Pioneer Pharmacy Degree College, India 2 University Department of Pharmaceutical Sciences, RTM Nagpur University, India *Corresponding author: Dhananjay Meshram, Department of Quality Assurance, Pioneer Pharmacy Degree College, India Received: March 20, 2017; Accepted: April 12, 2017; Published: April 19, 2017
Abstract A simple and rapid RP-HPLC method has been developed and validated for the simultaneous determination of Levocloperastine fendizoate and Chlorpheniramine maleate syrup formulation. Resolution of the analytes was achieved within 10min, employing a mixture of 10mM mobile phase Buffer (pH 6.5): Acetonitrile (50:50, % v/v) as isocratic mobile phase, pumped at 1.0mL/ min through a C18 column (5µm particle size). The detection wavelength for the analytes was 227nm. The system suitability parameters were found to be acceptable. The linearity of response (r2>0.999) in the appropriate ranges (from 50% up to 150% of the expected concentrations of the analytes in the formulations), method accuracy (RSD
