Development of a syngeneic mouse model for events related to ...

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Development of a syngeneic mouse model for events related to ...

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rat (10,11) ovarian surface epithelial cells and tumor cell lines and syngeneic C57BL6 .... DMEM either s.c. or i.p. underlying stromal tissue (Figure 1A and B).

Carcinogenesis vol.21 no.4 pp.585–591, 2000

Development of a syngeneic mouse model for events related to ovarian cancer

Katherine F.Roby1,5, Christopher C.Taylor2, Jeffrey P.Sweetwood2, Ying Cheng2, Judith L.Pace2, Ossama Tawfik3,4, Diane L.Persons3, Peter G.Smith2 and Paul F.Terranova2,4 1Department

of Anatomy and Cell Biology, 2Department of Molecular and Integrative Physiology, 3Department of Pathology and Laboratory Medicine and 4Department of Obstetrics and Gynecology, University of Kansas Medical Center, Center for Reproductive Sciences, Kansas City, KS 66160, USA 5To

whom correspondence should be addressed Email: [email protected]

Mouse ovarian surface epithelial cells (MOSEC) were obtained from virgin, mature mice by mild trypsinization and were repeatedly passaged in vitro. Early passage cells (