Dichloromethane Fraction of Laminaria japonica ... - Springer Link
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Dichloromethane Fraction of Laminaria japonica ... - Springer Link
Synthase and Cyclooxygenase-2 Expression in RAW 264.7. Cells via NF-κB Pathway. Ji-Young Lee,. 1. Min-Sup Lee,. 1. Ji-Woong Choi,. 1. Tai Sun Shin,. 2.
Dichloromethane Fraction of Laminaria japonica Ethanolic Extract Inhibits Lipopolysaccharide-Induced Nitric Oxide Synthase and Cyclooxygenase-2 Expression in RAW 264.7 Cells via NF-κB Pathway Ji-Young Lee,1 Min-Sup Lee,1 Ji-Woong Choi,1 Tai Sun Shin,2 Hee-Chul Woo,3 and Hyeung-Rak Kim1,4
Abstract—Strong anti-inflammatory activity has been found in Laminaria japonica dichloromethane fraction (LDF); however, the molecular mechanisms underlying its anti-inflammatory activity are not reported. Our results indicated that LDF inhibited LPS-induced nitric oxide and prostaglandin E2 production in a dose-dependent manner and suppressed the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in RAW 264.7 cells. Also, levels of pro-inflammatory cytokines such as tumor necrosis factor-α, interleukin (IL)-1β and IL-6 were remarkably reduced by LDF in LPS-treated RAW 264.7 cells. LDF greatly inhibited promoter activity of nuclear factor-κB (NF-κB) and translocation of NF-κB subunits by prevention of the degradation of inhibitor κB-α in LPS-treated RAW 264.7 cells (p
