Susan E. Ozanne,â¡ Dave O. Kleemann,§ Simon K. Walker,§ Janna L. Morrison,*. Song Zhang,* Beverley S. Muhlhäusler,* Malgorzata S. Martin-Gronert,â¡.
The FASEB Journal • Research Communication
Differential effects of maternal obesity and weight loss in the periconceptional period on the epigenetic regulation of hepatic insulin-signaling pathways in the offspring Lisa M. Nicholas,* Leewen Rattanatray,*,† Severence M. MacLaughlin,* Susan E. Ozanne,‡ Dave O. Kleemann,§ Simon K. Walker,§ Janna L. Morrison,* Song Zhang,* Beverley S. Muhlhäusler,* Malgorzata S. Martin-Gronert,‡ and Isabella C. McMillen*,1 *Sansom Institute for Health Research, School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia, Australia; †Discipline of Physiology, School of Molecular and Life Sciences, University of Adelaide, Adelaide, South Australia, Australia; ‡Institute of Metabolic Science, Metabolic Research Laboratories, University of Cambridge, Addenbrooke’s Hospital, Cambridge, UK; and §Turretfield Research Centre, South Australian Research and Development Institute, Rosedale, South Australia, Australia Our aim was to determine the effect of exposure to maternal obesity or to maternal weight loss around conception on the programming of hepatic insulin signaling in the offspring. We used an embryo transfer model in sheep to investigate the effects of exposure to either maternal obesity or to weight loss in normal and obese mothers preceding and for 1 wk after conception on the expression of hepatic insulin-signaling and gluconeogenic factors and key miRNAs involved in insulin signaling in the offspring. We found that exposure to maternal obesity resulted in increased hepatic miR-29b (P
