Differential expression and functional annotation of iPSC derived Human retinal pigment epithelial cells. Vikrant Singh1,Grace E. Liderwood2,Duncan E. Crombie2,Helena H. Liang2, Anthony Cook1, Alex Hewitt1, Alice Pébay2 1Menzies
Institute for Medical Research, University of Tasmania, TAS 7000, Australia of Surgery, University of Melbourne, VIC 3002, Australia
2Department
A.
Introduction Retinal degenerative diseases such as age-related macular degeneration (AMD) and other retinopathies lead to the impairment of photoreceptor cells, ultimately leading to blindness. Induced pluripotent stem cells (iPSCs) have generated great expectations in the field of regenerative medicine due to their ability to give rise to any cell type in the body. In the following research the total RNA from purified RPE cells is stabilized, extracted and ribosomal RNA is depleted. RNA integrity is confirmed with Bioanalyzer 2100 (Aligent) before undergoing library preparation and sequencing on an Illumina HiSeq 4000 was used to obtained 100-bp-pairedend reads at Australian Genome Research facility (AGRF). In this study we investigated differentially expressed gene and their functional annotation.
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Down-regulated
Up-regulated
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Objective To reveal the transcriptome analysis and molecular signature of iPSC derived human retinal pigment epithelial cells.
Methodology • Transcript abundance quantification and differential expression analysis of iPSCs derived RPE samples were estimated by using Kallisto and Sleuth respectively. • Functional annotation, classification and identification of enriched genes was examined using Consensus PANTHER-Classification system. • P-value was used to measure the significant of the gene-enrichment with in each biological process category.
