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Plant Mol Biol Rep DOI 10.1007/s11105-014-0825-y
BRIEF COMMUNICATION
Differential Expression of Two-Component System–Related Drought-Responsive Genes in Two Contrasting Drought-Tolerant Soybean Cultivars DT51 and MTD720 Under Well-Watered and Drought Conditions Nguyen Binh Anh Thu & Xuan Lan Thi Hoang & Thuy-Dung Ho Nguyen & Nguyen Phuong Thao & Lam-Son Phan Tran
# Springer Science+Business Media New York 2014
Abstract Two-component systems (TCSs) have been shown to participate in plant responses to drought. In this study, results of real-time quantitative PCR (RT-qPCR) of 26 selected dehydration-responsive TCS-related genes in roots and shoots of two Vietnamese soybean cultivars (DT51 and MTD720) with contrasting drought-tolerant phenotypes suggest a positive correlation between the number of droughtinducible TCS genes and their drought-tolerant ability. In addition, expression analyses of the roots and shoots indicated that DT51 and MTD720 had distinct drought-responsive TCS expression profiles, suggesting that expression of TCS-related genes are genotype and tissue dependent. Furthermore, nine TCS genes (GmHK07, 16, GmHP08, GmRR04, 16, 32, 34, GmPRR39, and 44) potentially associated with enhanced drought tolerance were identified. Particularly, GmRR34, showing its higher expression levels under both normal and drought conditions in DT51 roots versus MTD720 roots, might be a potential positive regulator of drought tolerance. On the other hand, GmPRR44 was highly recommended as a potential negative regulator of drought tolerance because it These authors Nguyen Binh Anh Thu and Xuan Lan Thi Hoang contributed equally to this work. Electronic supplementary material The online version of this article (doi:10.1007/s11105-014-0825-y) contains supplementary material, which is available to authorized users. N. B. A. Thu : X. L. T. Hoang : T.