Dec 9, 2002 - Gamma glutamyltransferase. (GGT) is a carboxypeptidase which cleaves. C-terminal glutamyl groups and transfers them to peptides and other ...
ISSN 0971-6777
JOURNAL OF CAMEL PRACTICE AND RESEARCH Volume 9
December
Number 2
2002
.... :'
,'.
In This Issue Anatomy Detection of apoptosis
Oiseases Muscular weakness
and anorexia
immunology Colostral immunoglobulin vs, subsequent
G concentrations
serumconcentrations
Infectious Diseases Bacterial microflora Study of coagulase
types of S.aureus
Mastitis caused by P.haemolytica
and
S.agalactiae Brucellosis
in camels in Ethiopia
Parasitology Impact of surra on camel husbandry Treatment
of cerebrospinal
nematodiasis
Physiology Distribution
of gamma glutamyltransferase
Effect of heat stress and dehydration Effect of hot and cold ambience Gluconeogenic
behaviour
Neurohypophyseal
hormone profiles
Osmolal and water clearances
WORLD CAMEL POPULATION · 2002
1 ,
2
Vo/ 9 No 2. P 97·99
DISTRIBUTION OF GAMMA GLUTAMYLTRANSFERASE ACTIVITY IN THE TISSUES OF THE CAMEL ( Came/us dromedarius) S.Nazifi. DepanmS",i
M.Aminlari
01 Ctrnic at Studies
School
and A.Haji
of Veterinary
Mohammadi
Me drc.n e. Shir az Uruvsr suv Srur a z . IRfI.N
ABSTRACT Tissue c.stnbuuon of gamma glutamyltransferase (GGT) (Came/us cromecer.uss The specific activity of enzyme was observed pylore of abomasum and spleen In decreasing order. respectively Keywords;
CamE,
gamma
g!ulamyltranslerase.
tissue
Gamma glutamyltransferase (GGT) is a carboxypeptidase which cleaves C-terminal glutamyl groups and transfers them to peptides and other suitable acceptors. The greatest amount of cellular GGT exists in the brush borders of renal and bile duct epithelia (Kaneko, 1989). Changes in serum GGT are a useful indicator of hepatobiliary disorders of cattle. sheep, goat and horse and cholestatic disorders of dog. Increases in urine GGT indicate toxic kidney damage (Braun et et. 1983; Kaneko, 1989). The distribution of GGT between the organs of normal animals has been studied in cows (Rico et et, 1977b; Schmid and Von Forstner, 1986), calves (Braun et al:-- 1978), horses (Rico et et, 1977a; Ford and Adam. 1981 ;'Blasslng et et. 1982) and dogs (Schmid and Von Forstner. 1986). Afzal and Saeed (1995) reported GGT activity to be highest in the kidney of camel, but in this study the number of camel organs and tissues was limited. Also. the activity of GGT was measured in IU/g tissue. Beriqourru et al (1997) repor ed that GGT activity was highest in the kidney of camel and the distribution of GGT in the liver, muscle and myocardium was similar to that of other domestic ruminants. The re is little recent fu rther information on the distribution of GGT activity in the tissues of the camel. SEND REPRINT Journal
was studied in 14 nealthy adult c arn e+s in kidney (medulla) liver. kidney (cortex).
The purpose of this investigation was to examine and compare the tissue distribution of GGT in camel The results of these studies will help to assess the role and significance of GGT in different tissues of came!. Serum enzymes are monitored routinely in camels to detect and assess damage to various organs. This study would assist the rational interpretation of serum GGT data in camel. Materials and Methods Fourteen apparently healthy camels (Camelus oromeoenusi, 3 to 6 years old. were slaughtered at the slaughter house located at Yazd province in the centre of Iran. Immediately after slaughter, 238 tissue samples were obtained from liver, brain. kidney (cortex and medulla), lymph node, lung, skeletal muscle', spleen, urinary bladder, heart (atrium and ventricle), rumen, reticulum. abomasum (pylore and fundus). prostate and ovary All samples. kept on ice, were transferred to the laboratory within 45 minutes, Tissues were separated, stripped from fat and extraneous materials washed a few times with physiological saline and then blotted. Tissue extracts were prepared by freezing 1 9 of the sample in liquid nitrogen, homogenising with a hand-homogeniser and suspending the
REOUEST TO S.NAZIFI
of Camel Practice
and Research
December
2002/97
The results showed that the activity and specific activity of GGT In different tissues were significantly different (p
