Divergent targets of glycolysis and oxidative phosphorylation ... - Nature

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Maria Orengo3; Lizzia Raffaghello2; Gianmario Sambuceti3. 1 CNR Institute of Molecular Bioimaging and Physiology (IBFM), Milan, Section of Genoa, Genoa, ...
Divergent targets of glycolysis and oxidative phosphorylation result in additive effects of metformin and starvation in colon and breast cancer

*Cecilia Marini1, *Giovanna Bianchi2, Ambra Buschiazzo3, Silvia Ravera4; Roberto Martella2; Gianluca Bottoni3; Andrea Petretto5; Laura Emionite6; Elena Monteverde3; Selene Capitanio3, Elvira Inglese5; Marina Fabbi7; Francesca Bongioanni3; Lucia Garaboldi3; Paolo Bruzzi8, Anna Maria Orengo3; Lizzia Raffaghello2; Gianmario Sambuceti3.

1

CNR Institute of Molecular Bioimaging and Physiology (IBFM), Milan, Section of Genoa, Genoa, Italy

2

Oncology lab, Istituto Giannina Gaslini, Genoa, Italy

3

Nuclear Medicine, Department of Health Sciences, University of Genoa and IRCCS AOU San Martino-IST,

Genoa, Italy 4

Biochemistry Lab, Department of Pharmacy, University of Genoa, Genoa, Italy

5

Core facility, Istituto Giannina Gaslini, Genoa, Italy

6

Animal facility, IRCCS AOU San Martino-IST, Genoa, Italy

7

Integrated oncological therapies, IRCCS AOU San Martino-IST, Genoa, Italy

8

Statistics and Epidemiology Unit, IRCCS AOU San Martino-IST, Genoa, Italy

Keywords: cancer metabolism, metformin, starvation, glycolysis, oxidative phosphorylation. The authors have no potential conflicts of interest to disclose. * Both CM and GB acted as first author. Address for correspondence: Cecilia Marini, MD, PhD, C/o Nuclear Medicine IRCCS AOU San Martino-IST, 160143- Genoa, Italy

Email: [email protected]

Suppl. Table 1: Body weight in grams and serum glucose level in each study group at the two different scan times Weight (grams) PET 1 PET 2

CTR

STS

MTF

MTF + STS

Serum glucose (mmol/L) PET 1 PET 2

CT26 (n=7)

18,60 ± 1,48

17,71 ± 1,23

4,26 ± 1,22

3,50 ± 2,59

4T1 (n=7)

16,17 ± 0,75

17,55 ± 0,56

4,71 ± 0,74

4,74 ± 0,88

CT26 (n=7)

16,20 ± 0,47

15,40 ± 1,94

3,58 ± 0,87

3,13 ± 1,00

4T1 (n=7)

13,45 ± 0,27

14,34 ± 0,58

4,19 ± 1,18

4,80 ± 2,55

CT26 (n=7)

15,91 ± 0,61

17,31 ± 1,05

4,24 ± 1,42

3,50 ± 1,21

4T1 (n=7)

16,36 ± 0,78

17,66 ± 0,78

4,40 ± 1,20

5,14 ± 1,48

CT26 (n=7)

13,08 ± 0.76

*

14,75 ± 0.94

4T1 (n=7)

13,74 ± 0.36

*

13,45 ± 0.59

*=p