RESEARCH ARTICLE
DNA Detection of Schistosoma japonicum: Diagnostic Validity of a LAMP Assay for Low-Intensity Infection and Effects of Chemotherapy in Humans Jing Xu1☯, Zhi-Xun Guan1☯, Bo Zhao1, Yan-Yan Wang1, Yun Cao1, Hui-Qin Zhang1, Xing-Quan Zhu2, Yong-Kang He3, Chao-Ming Xia1* 1 Department of Parasitology, Medical College of Soochow University, Suzhou, Jiangsu Province, China, 2 State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, China, 3 Hunan Provincial Institute of Schistosomiasis Control, Yueyang, Hunan Province, China ☯ These authors contributed equally to this work. *
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[email protected] OPEN ACCESS Citation: Xu J, Guan Z-X, Zhao B, Wang Y-Y, Cao Y, Zhang H-Q, et al. (2015) DNA Detection of Schistosoma japonicum: Diagnostic Validity of a LAMP Assay for Low-Intensity Infection and Effects of Chemotherapy in Humans. PLoS Negl Trop Dis 9(4): e0003668. doi:10.1371/journal.pntd.0003668 Editor: Aaron R. Jex, University of Melbourne, AUSTRALIA Received: October 7, 2014 Accepted: March 2, 2015 Published: April 13, 2015 Copyright: © 2015 Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper. Funding: This study was funded by A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institution (No.YX13400214); the National Basic Research Program of China(973 Program, Grant No. 2007CB513100); Key Laboratory of Control and Prevention of Parasitic Disease of Healthy Ministry, No. wk014-001). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Abstract Background Schistosomiasis has decreased significantly in prevalence and intensity of infection in China, thus more accurate and sensitive methods are desperately needed for the further control of schistosomiasis. The present work aimed to assess the utility of the loop-mediated isothermal amplification (LAMP) for detection of light intensity infection or false-negative patients and patients post-treatment, targeting the highly repetitive retrotransposon SjR2 of Schistosoma japonicum.
Methodology/ Principal Findings LAMP was first assessed in rabbits with low intensity infection (EPG
