Figure 2. Relative expression of clock genes in cultured liver treated with ghrelin and the antagonist of ghrelin receptor ([D-Lys3] GHRP-6) during 1 h. Data are ...
GHRELIN MODIFIES CLOCK GENES EXPRESSION IN THE LIVER OF GOLDFISH VIA PLC-PKC PATHWAY Aída Sánchez-Bretaño, A.M. Blanco, A.L. Alonso-Gómez, M.J. Delgado, E. Isorna.
Department of Physiology, Faculty of Biology, Complutense University of Madrid, Spain. Experimental designs Introduction LIVER: main link between metabolism and circadian system [1,2,3,4]
ZT 0 ZT2
n=6
GHRELIN
15 mg liver/1 ml DMEM/well 24L (x lux), 21 °C
LIVER SAMPLING Ghrelin concentrationresponse study
Proximal gastrointestinal tract (main site of ghrelin synthesis)
[2,4]
Does ghrelin regulate clock genes expression in cultured liver? Which is the intracellular pathway involved?
Results 1 Ghrelin action on clock genes expression in cultured liver gper1a
PKC inhibitor
PLC inhibitor
PKC inhibitor
grev-erbα
gbmal1a
PLC inhibitor
PKA inhibitor
Figure1. Concentration-response effects of clock genes expression in cultured liver treated with ghrelin (GRL) for 1 or 5 h. Data are shown as mean±s.e.m. (n=6) in relative units (ΔΔCt method). Different letters indicate significant differences among groups (p