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iboth dissolved and adsorbed organic pools in seo•-wter,. A.n previous work we ... RAVI1 YOU LICENSED TECSXOLOGIES (E.G., SOFTWARE) TEAT WERE DEVELOPED WITH ONR ... Release of dissolved free and combined amino acids by ...
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3. REOT TYPE AND DATES •CVERID

:EPCIT DATE

SJuly 28, 1993

Final Report

1 May 1990-30 Ju-ne 1993

4. TITLE AND •UZIT'TLE

S. FUNDING NL.*Ai5;S

Regulation of Attached Bacterial Growth by Adsorbed Proteins

N00014-90-J-1973

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i. AUTHOR3q

David L. KIrchman

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College of Marine Studies University of Delaware Lewes, DE 19958

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Office of Naval Research 800 N. Quincy Street Arlirtton, VA 22217-5000

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The general objective of this project was to exam[ine one component of the I ;primary biofilm, protein, which has been hypothe'ized to be a malor ccn.luonient or previous work we found iboth dissolved and adsorbed organic pools in seo•-wter, I that surface energy was important in determining adsorption of protein to surf1ý'-! In the last )in seawater and degradation rates by bacteria And bacterial growth, A.n

work, wle examined the chemical nature of actual proteln ii rawitir phase of this •uslng 'HPLC techniques which wedevelop-d, stan,-dard chomicai arsays, And bioas. protein. We showed that protein in seawater Is not th" snme choincally aq cellular s'nwator prot.o[n P- if it were glvcolated ord nearly all Bacteria apTear to treat

degrade it at much lower rates than unmodifled, ho-'4ed

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rictfor5 anrd becomes lesn; easily hylriiny:-,d by b,:trtao n abtotic reactionr anpear to include aOr","r. :1 'r of to explain the ,rowth d' ',in .....C.t'ryr7 cr ,on c T atter which I- hip-1 r 7.....

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FINAL REPMRT QUESTIONNAIRE (for ONR use only)

David L. Kirchman

Principal Investigator Name: Institution:

University of Delaware

Project Title: Regulation of Attached Bacterial Growth by Adsorbed Protein Number of ONR supported Papers published in refereed journals:___ Papers ur reports in non-refereed publicationst Books or book chapters published: __ Number of ONR supported patents/inventions

None

led1.: Patent name and number_

Granted:

RAVI1 YOU LICENSED TECSXOLOGIES (E.G., SUPPORT?

IF SO,

SOFTWARE) TEAT WERE DEVELOPED WITH ONR

PLEASE DESCRIBE ON A SEPARATE SHEET.

RAVE YOU DEIELOPED INDUSTRIAL/CORPORATE CONNECTIONS BASED ON YOUR OUR IF SO, PLEASE DESCRIBE ON A SEPARATE SHEET. SIUPPORTED RESEARCH?

Number of presentations. Invitds

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Contributed3 Trainee Data eonl'

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No. Grad.

Students:

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FEMALE

NON-US CITIZEN

3

No.

Postdoctorals:

1

No.

Undergradu.ates:

1

1 1

AWARDS/HONORS TO PI AND/oR TO ME,4M3ERS OF 2I'S RESEARCH GnOUP (pleioc describe): r and p

P.I. was appointed editor of [t-nn]a Division 11of American Society of M1crobiology.

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List of Papers in Refereed Journals Published During Project May 1990- June 1993. "'Montgomery, M.T., N.W. Welschmeyer, and D.L. Kirchxnan. 1990. A simple assay for chitin: Application to sediment trap samples from the Subarctic Pacific. Mar. EcoL. Prog. Ser. 64: 301-308. "Nagata, T. and D.L. Kirchman. 1990. Filtration-induced release of dissolved free amino acids: application to cultures of marine protozoa. Mar. Ecol. Prog. Ser. 68: 1-5. "Samuelsson, M-0. and D.L. Kirchman. 199. Degradation of adsorbed protein by attached bacteria in relationship to surface hydrophobicity. Appi. Environ. Microbiol. 56: 3643-3648. *Keil, R.G. and D.L. Kirchman. 1991. Dissolved combined amino acids in marine waters as determined by a vapor-phase hydrolysis method. Mar. (Them. 33: 243-259. "Nagata, T. and D.L. Kirchman. 1991. Release of dissolved free and combined amino acids by bacteriovorous marine flagellates. Limnol. Oceanogr. 36(3): 433-443. *Keil, R.G. and D.L. Kirchman. Contribution of dissolved free amino acids and ammnonium to the nitrogen requirements of heterotrophic bacterioplankion. Mar. FEcol. Prog. Scr. 73: 1-10. Logan, B.E. and D.L Kirchinan. Increased uptake of dissolved organics by marine bacteria as a function of fluid motion. Mar. Biol. 111: 175-181. *Keil, R.G. and D.L. Kircbman. 1992 Bacterial degradation of methylated protein and uniformly labelled protein. Appl. Environ. Mic-robiol. 58: 1374-13715. "Montgorniery, M.T. and D.L. Kirchm an. 1993. Role of chitin-binding pro!c ins in the specific attachment of the marinc bacterium Vibrio harveyi to chitin. A~pp. Environ. Microbio). 59: 373-379. *Keil, R.G. and 131. Kirchman. 1993. Dis.solved combined amrino acids, chemical form and utý]l`itaýon by mairinc b-acteria. Lininol. Oceano~lr. In press.. *Kc.0, R.G. and D.L. Kirchinan. Abiotic transformaition of labile protein Lo refractory

protein in seawiater. Mar. Chem. In prcss3. "~Shicients or postdoc',s in P.T.':; lab (DI." Krchman)

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as the first page and mail two copies (including the postcard labelled DTIC FORM 50) to: Defense Technical Information Center Building 5, Cameron Station Alexandria, VA 22314 This allows other investigators to obtain copies of your report directly from DTIC. DTIC will fill out the postcard DTIC ACCESSION NOTICE (oTIC FORM 50) and return it to

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(e)

Director Chemical and Biological Sci Div Army Research Office P. 0. Box 12211 Research Triangle Park, NC 27709

(b)

Administrative Grants Officer ONR Resident Representative (address varies - see copy of your grant/contract)

(f)

Life Sciences Directorate Air Force Office of Scientific Res Bolling Air Force Base Washington, DC 20332

(c)

Director, Applied Research Directorate ONR Code 12 800 N. Quincy Street Arlington, VA 22217-5000

(g)

Director Naval Research Laboratory Technical Information Div Code 2627 Washington, DC 20375

(d)

Director Office of Naval Technology Code 22 800 N. Quincy Street

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A&co,-1-

Arlington, VA 22217-5000

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