iboth dissolved and adsorbed organic pools in seoâ¢-wter,. A.n previous work we ... RAVI1 YOU LICENSED TECSXOLOGIES (E.G., SOFTWARE) TEAT WERE DEVELOPED WITH ONR ... Release of dissolved free and combined amino acids by ...
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3. REOT TYPE AND DATES •CVERID
:EPCIT DATE
SJuly 28, 1993
Final Report
1 May 1990-30 Ju-ne 1993
4. TITLE AND •UZIT'TLE
S. FUNDING NL.*Ai5;S
Regulation of Attached Bacterial Growth by Adsorbed Proteins
N00014-90-J-1973
D T iC
i. AUTHOR3q
David L. KIrchman
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College of Marine Studies University of Delaware Lewes, DE 19958
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Office of Naval Research 800 N. Quincy Street Arlirtton, VA 22217-5000
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The general objective of this project was to exam[ine one component of the I ;primary biofilm, protein, which has been hypothe'ized to be a malor ccn.luonient or previous work we found iboth dissolved and adsorbed organic pools in seo•-wter, I that surface energy was important in determining adsorption of protein to surf1ý'-! In the last )in seawater and degradation rates by bacteria And bacterial growth, A.n
work, wle examined the chemical nature of actual proteln ii rawitir phase of this •uslng 'HPLC techniques which wedevelop-d, stan,-dard chomicai arsays, And bioas. protein. We showed that protein in seawater Is not th" snme choincally aq cellular s'nwator prot.o[n P- if it were glvcolated ord nearly all Bacteria apTear to treat
degrade it at much lower rates than unmodifled, ho-'4ed
that cellular
protein
introduced
Into
"fre.h" protein. iS
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rictfor5 anrd becomes lesn; easily hylriiny:-,d by b,:trtao n abtotic reactionr anpear to include aOr","r. :1 'r of to explain the ,rowth d' ',in .....C.t'ryr7 cr ,on c T atter which I- hip-1 r 7.....
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FINAL REPMRT QUESTIONNAIRE (for ONR use only)
David L. Kirchman
Principal Investigator Name: Institution:
University of Delaware
Project Title: Regulation of Attached Bacterial Growth by Adsorbed Protein Number of ONR supported Papers published in refereed journals:___ Papers ur reports in non-refereed publicationst Books or book chapters published: __ Number of ONR supported patents/inventions
None
led1.: Patent name and number_
Granted:
RAVI1 YOU LICENSED TECSXOLOGIES (E.G., SUPPORT?
IF SO,
SOFTWARE) TEAT WERE DEVELOPED WITH ONR
PLEASE DESCRIBE ON A SEPARATE SHEET.
RAVE YOU DEIELOPED INDUSTRIAL/CORPORATE CONNECTIONS BASED ON YOUR OUR IF SO, PLEASE DESCRIBE ON A SEPARATE SHEET. SIUPPORTED RESEARCH?
Number of presentations. Invitds
g
QNRPrgq~e,
5
3
.
Contributed3 Trainee Data eonl'
L.
thgae re TOTAL
No. Grad.
Students:
_*'1A
=.
_tli. MINORITY
FEMALE
NON-US CITIZEN
3
No.
Postdoctorals:
1
No.
Undergradu.ates:
1
1 1
AWARDS/HONORS TO PI AND/oR TO ME,4M3ERS OF 2I'S RESEARCH GnOUP (pleioc describe): r and p
P.I. was appointed editor of [t-nn]a Division 11of American Society of M1crobiology.
EVyuirlmont
>$NIo5ne None
purchanod on .rf
t
(rý-!hr Anj
noi'-r
ltzt
P.1.
was Chlair of
on of itc:a c
List of Papers in Refereed Journals Published During Project May 1990- June 1993. "'Montgomery, M.T., N.W. Welschmeyer, and D.L. Kirchxnan. 1990. A simple assay for chitin: Application to sediment trap samples from the Subarctic Pacific. Mar. EcoL. Prog. Ser. 64: 301-308. "Nagata, T. and D.L. Kirchman. 1990. Filtration-induced release of dissolved free amino acids: application to cultures of marine protozoa. Mar. Ecol. Prog. Ser. 68: 1-5. "Samuelsson, M-0. and D.L. Kirchman. 199. Degradation of adsorbed protein by attached bacteria in relationship to surface hydrophobicity. Appi. Environ. Microbiol. 56: 3643-3648. *Keil, R.G. and D.L. Kirchman. 1991. Dissolved combined amino acids in marine waters as determined by a vapor-phase hydrolysis method. Mar. (Them. 33: 243-259. "Nagata, T. and D.L. Kirchman. 1991. Release of dissolved free and combined amino acids by bacteriovorous marine flagellates. Limnol. Oceanogr. 36(3): 433-443. *Keil, R.G. and D.L. Kirchman. Contribution of dissolved free amino acids and ammnonium to the nitrogen requirements of heterotrophic bacterioplankion. Mar. FEcol. Prog. Scr. 73: 1-10. Logan, B.E. and D.L Kirchinan. Increased uptake of dissolved organics by marine bacteria as a function of fluid motion. Mar. Biol. 111: 175-181. *Keil, R.G. and D.L. Kircbman. 1992 Bacterial degradation of methylated protein and uniformly labelled protein. Appl. Environ. Mic-robiol. 58: 1374-13715. "Montgorniery, M.T. and D.L. Kirchm an. 1993. Role of chitin-binding pro!c ins in the specific attachment of the marinc bacterium Vibrio harveyi to chitin. A~pp. Environ. Microbio). 59: 373-379. *Keil, R.G. and 131. Kirchman. 1993. Dis.solved combined amrino acids, chemical form and utý]l`itaýon by mairinc b-acteria. Lininol. Oceano~lr. In press.. *Kc.0, R.G. and D.L. Kirchinan. Abiotic transformaition of labile protein Lo refractory
protein in seawiater. Mar. Chem. In prcss3. "~Shicients or postdoc',s in P.T.':; lab (DI." Krchman)
4
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o,
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OTiC
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End (1)
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