The common octopus, Octopus vulgaris, is currently considered as excellent ... (i.e. limited movement and tank sharing) in the common octopus O. vulgaris. For.
Edinburgh International Conference Centre (EICC) 1
Edinburgh, Scotland 20-23 September, 2016
Organised by the European Aquaculture society with the cooperation and support of Marine Scotland, part of the Scottish Government, and The Marine Alliance for Science and Technology for Scotland AE2016 Gold Sponsor
www.easonline.org
74 TRYPTOPHAN AND METHIONINE DIETARY SUPPLEMENTATION EVOKES DISTINCT RESPONSES OF IMMUNE-RELATED GENES IN THE GUT OF EUROPEAN SEABASS (Dicentrarchus labrax L.) FOLLOWING AN INFLAMMATORY INSULT R. Azeredo1,2*, 0. 0DFKDdo1,3, F.A. Guardiola1,4, R. &erezuela4, A. Afonso1,3, +. 3eres1, A. Oliva-Teles1,2, 0.A. (sWeEan4 and %. &osWas1 &enWro ,nWerdisFiSlinar de ,nvesWiJaomo 0arinKa e APEienWal &,,0AR , 8niversidade do 3orWo, Rua dos %raJas 2 , 4-123 3orWo, 3orWuJal. (-Pail PlePe#FiiPar.uS.SW 2 'eSarWaPenWo de %ioloJia, FaFuldade de &irnFias da 8niversidade do 3orWo F&83 , 41 - 3orWo, 3orWuJal. 3 ,nsWiWuWo de &irnFias %ioPpdiFas AEel 6alazar ,&%A6-83 , 8niversidade do 3orWo, Rua de -orJe 9iWerEo Ferreira n 22, 4-313 3orWo, 3orWuJal. 4 'eSarWPenW of &ell %ioloJ\ and +isWoloJ\. FaFulW\ of %ioloJ\, &aPSus ReJional de ([FelenFia ,nWernaFional ³&aPSus 0are 1osWruP´, 8niversiW\ of 0urFia, 31 0urFia, 6Sain. 1
Introduction 7HOHRVWÀVKLPPXQHV\VWHPFRPSULVHVWKHVRFDOOHGJXWDVVRFLDWHGO\PSKRLGWLVVXH *$/7 ZKLFKUHVSRQVHVDUHPRVWO\ PHGLDWHG E\ UHVLGHQW O\PSKRF\WHV GHQGULWLF FHOOV PDFURSKDJHV DQG DQWLPLFURELDO SHSWLGHV 5RPERXW et al. $V QXWULHQWVDEVRUEDQFHLVFDUULHGRXWLQWKHJXWHSLWKHOLXPGLHWDU\WUHDWPHQWVPD\PRGXODWHORFDOLPPXQHUHVSRQVHV7KH LQYROYHPHQWRIVRPHDPLQRDFLGVRQVHYHUDOLPPXQHVLJQDOOLQJSDWKZD\VKDVORQJEHHQUHFRJQL]HG /LHWDO. EXW UHSRUWVRIWKHLUGLUHFWHIIHFWVRQÀVKKHDOWKDUHVWLOOVFDUFH7KHSUHVHQWVWXG\IRFXVHGRQWKHHIIHFWVRIGLHWDU\VXSSOHPHQWDWLRQ ZLWKWU\SWRSKDQ 753 RUPHWKLRQLQH 0(7 RQWKHJXWLPPXQHUHODWHGJHQHH[SUHVVLRQRI(XURSHDQVHDEDVVDIWHUDQ LQÁDPPDWRU\LQVXOW Material and methods (XURSHDQVHDEDVV Dicentrarchus labrax J ZHUHPDLQWDLQHGLQDUHFLUFXODWHGVHDZDWHUV\VWHP 7HPSHUDWXUH & 6DOLQLW\ SSW 3KRWRSHULRG KGDUN KOLJKW )LVKZHUHGLVWULEXWHGLQWRWDQNV O Q DQGGXSOLFDWH JURXSVRIWKHVHÀVKZHUHIHGWKUHHH[SHULPHQWDOGLHWVIRUGD\V DFRQWUROGLHW &75/ IRUPXODWHGWRPHHWDPLQRDFLG UHTXLUHPHQWVDQGWKH&75/GLHWVXSSOHPHQWHGZLWK753RU0(7DWðWKHUHTXLUHPHQWOHYHO$WWKHHQGRIWKHIHHGLQJWULDO ÀVKZHUHVXEMHFWHGWRLQÁDPPDWLRQE\LQWUDSHULWRQHDOLQMHFWLRQRI89NLOOHGPhotobacterium damselaeVXEVSpiscicida Phdp 6&)8PO )LVKLQMHFWHGZLWK+DQN·V%DODQFHG6DOW6ROXWLRQVHUYHGDVFRQWUROV6L[ÀVKIRUHDFKH[SHULPHQWDO JURXSZHUHVDPSOHGDWRUKSRVWLQMHFWLRQ SL )LVKZHUHVDFULÀFHGE\DQDHVWKHVLDZLWKSKHQR[\HWKDQRODQGVDPSOHV RIWKHSRVWHULRULQWHVWLQH 3, ZHUHFROOHFWHGIURPHDFKDQLPDODQGVWRUHGDW&XQWLOIXUWKHUDVVD\HG6DPSOHVZHUH SRROHGE\VLPXOWDQHRXVO\KRPRJHQL]LQJWKH3,RIWZRDQLPDOVUHVXOWLQJLQDQDO\WLFDOUHSOLFDWHVIRUHDFKH[SHULPHQWDO JURXS Q 7KHH[SUHVVLRQRILPPXQHUHODWHGJHQHVZDVHYDOXDWHGLQWKH3,E\UHDOWLPHTXDQWLWDWLYH3&5 Results $ONDOLQHSKRVSKDWDVH alp LQWHUOHXNLQ il8 DQGVXSHUR[LGHGLVPXWDVH sod P51$OHYHOVZHUHLQFUHDVHGDWKSLLQ ÀVKIHG753GLHWFRPSDUHGWRÀVKIHGHLWKHU&75/RU0(7GLHWVVDPSOHGDWWKHVDPHWLPHDVZHOODVÀVKIHG753GLHW VDPSOHGDWKSL )LJXUH Casp1 FDVSDVH P51$OHYHOVZHUHDOVRKLJKHULQ753JURXSFRPSDUHGWRJURXSVIHG 753RU0(7GLHWVUHVSHFWLYHO\DWKRUKSL$WKSLalpZDVXSUHJXODWHGLQ0(7IHGÀVKFRPSDUHGWRWKH&75/ JURXS )LJXUH Discussion and conclusions 7KRXJKPRVWRIVWXGLHVFRQFHUQLQJGLHWDU\753DUHIRFXVHGRQQHXURHQGRFULQHIXQFWLRQVWKHYDVWVLJQDOOLQJSDWKZD\V HVWDEOLVKHGEHWZHHQWKHQHXURHQGRFULQHDQGWKHLPPXQHV\VWHPVVXJJHVWVWKDWWKHLPPXQHUHVSRQVHPD\EHPRGXODWHGE\ PRGLÀFDWLRQVRIWKHRUJDQLVP·V753SRRO7KHSUHVHQWVWXG\UHSRUWVORFDOXSUHJXODWLRQRILPPXQHUHODWHGJHQHVGXULQJDQ LQÁDPPDWRU\UHVSRQVHLQ(XURSHDQVHDEDVVIHG753VXSSOHPHQWHGGLHWVUHYHDOLQJDFOHDUVWLPXODWRU\HIIHFWRIWKLVDPLQR DFLGRQ*$/7ZKLOH0(7SRRUO\DIIHFWHGLW2WKHUUHVXOWVRIWKLVVWXG\ QRWSUHVHQWHG VKRZHGHOHYDWHGSURGXFWLRQRI EUDLQPRQRDPLQHVFRQÀUPLQJWKHVWURQJPRGXODWRU\HIIHFWVRI753RQWKHEUDLQDQGVXJJHVWLQJWKDWLWVLQÁXHQFHRQJXW LPPXQLW\PLJKWEHPHGLDWHGE\WKHK\SRWKDODPXVSLWXLWDU\LQWHUUHQDOD[LV
75
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