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Methods: Use 9-month-old APP/PS1 double transgenic rats according to the random assignment method divided into model group, the EA group and drug(AChE) group within 11 rats in each group, use the same months old rats with brood recessive gene as control group. Treatment was applied to “Baihui”(GV20) and“yongquan”(KI1) for 15mins, once every 2 days for 5weeks; For the drug group, 0.92 ml/g of acetylcholine enzyme was given by gavage, once a day. Morris water maze test the ability of learning-memorize in rats and space exploration ability, take the brain hippocampus to make immunohistochemistry and transmission electric lens and observe. Results: Morris water maze test result shows that the model group compared with control group and EA group has statistically difference (P < 0.05); Space exploration experiment: model group in the region of the original platform quadrant (the third quadrant) activity time significantly lower than the control group and EA group(P < 0.05), Immunohistochemitry results shows that in model group and drug group has A stain on hippocampal; On transmission electron microscopy (sem) results showed that on both model group and drug group has senile plaque. Conclusion: 10-month-old APP/PS1 transgemic rats has senile plaque; EA therapy can improve the APP/PS1 double transgenic rats learning-memorize ability, have good adjustment function to the hippocampus. These performance of EA could be improved AD’s one of the mechanism of the behavior of learning and memorize ability. Contact: Jiawula Abulizi,
[email protected] http://dx.doi.org/10.1016/j.imr.2015.04.043 P1.037 Effect of Electro-acupuncture Intervention on Learning-memory Ability and Hippocampus Ultrastructure in APP/PS1 Double Transgenic Rats Menghan lu 1 , Jiawula Abulizi 2 , Xin Wang 2 , Yuping Mo 2 , Xiangbo Han 2 , Jin Cao 2 , Haijiang Yao 2 , Liangyu Song 2 , Yuan Zhou 2 , Qianfeng Shao 2 , Wei Lv 2 , Quankai Jing 2 1 2
Beijing University of Chinese Medicine College of Acupuncture and Massage,BUCM
Purpose: To investigate the effects of the electroacupuncture (EA) for APP/PS1 double transgenic rat on both spatial learning- memorize behavior and hippocampal ultrastructure. Methods: Divide 36 4-month-old APP/PS1 double transgenic rats into 3 groups, which are the model group, the EA group and the drug(AChE) group, by using the random assignment method. 12 4-month-old rats with brood recessive gene were taken as the control group. For the EA group, “Baihui”(GV20) and “yongquan”(KI1) were given treatment for 15 minutes every other day, lasting for 5 weeks. Gavaging with 0.92 ml/g of acetylcholine enzyme was given to the drug group. Test the learning-memorize ability and space exploration ability of the rats by using the Morris water maze. Observe slices of
ICCMR 2015 Poster Presentation Abstracts
the brain hippocampus CA1 area with transmission electron microscope. Results: According to the results of the Morris water maze test, there is statistical difference between the model group and the control group(P
