Effects of extracts from Panax notoginseng and Panax ginseng fruit on ...
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Effects of extracts from Panax notoginseng and Panax ginseng fruit on ...
vein endothelial cells (HUVECs) was used to observe the effect of extracts from Panax notoginseng (EPN) and Panax ginseng fruit (EPGF) on cell proliferation,.
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EXPERIMENTAL RESEARCH Effects of Extracts from Panax Notoginseng and Panax Ginseng Fruit on Vascular Endothelial Cell Proliferation and Migration in vitro LEI Yan (雷
燕)1, GAO Qian (高
倩)2, and CHEN Ke-ji (陈可冀)1
ABSTRACT Objective: To study the effects of extracts from Panax notoginseng (EPN) and Panax ginseng fruit (EPGF) on the proliferation and migration of human umbilical vein endothelial cells (HUVECs) in vitro . Methods: Cell proliferation was determined using an MTT method with a cultured HUVECs model cell cycle analyzed by cytometry. The effect on endothelial cell migration was investigated using an agarose scraping method. The content of vascular endothelial growth factor (VEGF) in the supernate was determined by enzyme-linked immunosorbent assay (ELISA). The VEGF mRNA expression of vascular endothelial cells (VECs) with different concentrations of EPN and EPGF was examined by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: EPN and EPGF can promote the proliferation of VECs and the secretion of VEGF from HUVECs. It can increase the cell population significantly in the S phase to (15.22±1.33) % in the 50 mg/L dose group (P
