^9. /L. ) 6. 8. 10. 12. 14. Monocyte percentage. Perc entage m onoc y tes of total w hite blood c ells. 0. 1. 2. 3. 4. Lymphocyte count¬. Ly m phoc y te c ount (x.
Supplementary material
Effects of oral butyrate supplementation on inflammatory potential of circulating peripheral blood mononuclear cells in healthy and obese males Maartje C.P. Cleophas1,2, Jacqueline M. Ratter1,2,3, Siroon Bekkering1,2, Jessica Quintin5, Kiki Schraa1, Erik S. Stroes4, Mihai G. Netea1,2,6, Leo A.B. Joosten1,2,7
1 Department of Internal Medicine, 2 Radboud Institute for Molecular Life Sciences (RIMLS),
Radboud university medical center, Nijmegen, the Netherlands 3 Nutrition, Metabolism and Genomics Group, Division of Human Nutrition, Wageningen
University, Wageningen, the Netherlands 4 Department of Vascular Medicine, Academic Medical Center, University of Amsterdam,
Amsterdam, the Netherlands 5 Immunology of Fungal Infections group, Department of Mycology, Institut Pasteur, Paris, France 6 Department for Genomics & Immunoregulation, Life and Medical Sciences Institute (LIMES),
University of Bonn, 53115 Bonn, Germany 7 Department of Medical Genetics, Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-
Napoca, Romania
B
A
14
Monocyte count
0.8
Percentage monocytes of total white blood cells
Monocyte count (x10^9/L)
1.0
0.6 0.4 0.2
Percentage lymphocytes of total white blood cells
Lymphocyte count (x10^9/L)
8
60
Lymphocyte count¬
3
2
1
Lymphocyte percentage
40
20
0
0
D
E
80
Neutrophil count¬
*
6
Percentage neutrophils of total white blood cells
Neutrophil count (x10^9/L)
10
D
C
8
12
6
0.0
4
Monocyte percentage
4
2
Neutrophil percentage
70 60 50 40 30
0
BL Lean
4w
BL
4w
MetSyn
BL Lean
4w
BL
4w
MetSyn
Supplemental Figure 1. Blood cell counts before and after butyrate supplementation Absolute counts and percentages of monocytes, lymphocytes and neutrophils were measured before and after butyrate supplementation. Baseline (BL) neutrophil counts were significantly higher in the MetSyn group compared to the lean individuals (panel E). No statistical significance was detected when comparing values before (BL) and after butyrate supplementation (4w).
