Effects of Support and Synthetic Procedure for Sol-Immobilized Au ...

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Jun 20, 2016 - and the kind of support affect the catalysts' activity. ... Keywords: gold catalyst; CeO2; ZrO2; TiO2; glucose; oxidation; Au sol-immobilization; ...
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Effects of Support and Synthetic Procedure for Sol-Immobilized Au Nanoparticles Michela Signoretto 1, *, Federica Menegazzo 1 , Alessandro Di Michele 2 and Ermelinda Fioriniello 1 1 2

*

Department of Molecular Sciences and Nanosystems, Ca’ Foscari University Venice and INSTM-RU Ve, Via Torino 155, Venezia Mestre 30170, Italy; [email protected] (F.M.); [email protected] (E.F.) Physics and Geology Department, University of Perugia, Via Pascoli, Perugia 06123, Italy; [email protected] Correspondence: [email protected]; Tel.: +39-041-234-8650

Academic Editors: Leonarda F. Liotta and Salvatore Scirè Received: 15 March 2016; Accepted: 6 June 2016; Published: 20 June 2016

Abstract: New gold catalysts supported on CeO2 , ZrO2 and TiO2 were synthesized by two different techniques: deposition-precipitation and colloidal method. The role of the surfactant (PVA, PVP, THPC) was also investigated. The catalysts were tested in the oxidation of glucose to gluconic acid, in aqueous environment and under mild conditions (60 ˝ C and atmospheric pressure). TEM and SEM analyses have shown that the small size of gold nanoparticles is a necessary condition, but not sufficient for a good conversion. In fact, for an active sample, we have verified that the excess of surfactant must be removed because it would coat the surface of the catalyst. The surfactant, however, should not be completely eliminated, since it has the fundamental role of stabilizing the sample preventing nanoparticles from aggregation. It was evidenced that both the synthetic approach and the kind of support affect the catalysts’ activity. In fact, by focusing on the three different supports, with all the preparation methods, the ceria has proved to be the best support. This is due to its ability to obtain small gold nanoparticles and to its ability to accumulate oxygen. The most appropriate synthesis methodology proved to be the colloidal method with PVA. Recyclability issue was investigated too. Keywords: gold catalyst; CeO2 ; ZrO2 ; TiO2 ; glucose; oxidation; Au sol-immobilization; PVA; PVP; THPC

1. Introduction The catalytic activity of a gold-based heterogeneous catalyst depends on the nature of the support and in particular on the size of the gold nanoparticles deposited on it, which in turn depends on the synthetic methodology. The size of the nanoparticles is of significant importance, since in many studies it was evidenced a correlation of inverse proportionality between the activity of the catalyst and the size of the gold nanoparticles [1]. The techniques for the preparation of gold nanoparticles are various. Almost all use chloroauric acid (HAuCl4 ¨3H2 O) as the metal precursor. The most used techniques in order to obtain an efficient heterogeneous catalyst are: impregnation (IMP), deposition-precipitation (DP) and colloidal methods. As regard as gold samples, dry impregnation is the first method historically used. The procedure involves filling the pores of the support by adding an aqueous HAuCl4 solution to form a wet paste, which is then dried and calcined. However, this technique does not allow obtaining high gold dispersion. In fact, it leads to nanoparticles of size above 30 nm, due to sintering during the heat treatment, promoted by the Cl´ ions still present on the sample [2]. Therefore, the gold-based catalysts prepared by this technique have a low catalytic activity [3]. An alternative synthetic approach for Au loading has been recently reported [4,5], namely a liquid phase reductive deposition (LPRD).

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The method consists of mixing(LPRD).  a solution ofmethod  HAuCl4consists  with a solution of NaOH. The of  resulting phase  reductive  deposition  The  of  mixing  a  solution  HAuClsolution 4  with  a  3+ ions. Then the appropriate was aged for 24 h, in the dark, to complete the hydroxylation of Au solution  of  NaOH.  The  resulting  solution  was  aged  for  24  h,  in  the  dark,  to  complete  the  3+ ions. Then the appropriate amount of support was added to the solution. In  amount of support was added to the solution. In LPRD, unlike IMP, a washing procedure is carried hydroxylation of Au out, in order to eliminate residual chloride. DP is a widely used procedure for the preparation of LPRD, unlike IMP, a washing procedure is carried out, in order to eliminate residual chloride. DP is  gold-based catalysts supported on metal oxides. In fact, this method works very well with support a widely used procedure for the preparation of gold‐based catalysts supported on metal oxides. In  whose isoelectric is greater than 5, such as magnesia, alumina, point  titania, and ceria. In as  a fact,  this  method point works  very  well  with  support  whose  isoelectric  is zirconia greater  than  5,  such  typical procedure, after the addition of the support to an aqueous solution of HAuCl4 ¨3H2 O, the pH of magnesia, alumina, titania, zirconia and ceria. In a typical procedure, after the addition of the support  the suspension is brought to a fixed4∙3H value by the addition of NaOH. The whole is heated under stirring, to an aqueous solution of HAuCl 2O, the pH of the suspension is brought to a fixed value by the  + and Cl−  before washing to remove the Na+ and Cl´ ions, drying and calcining in air. This basic approach addition of NaOH. The whole is heated under stirring, before washing to remove the Na has many variables such as for example the synthesis temperature and the pH. In general the pH is ions, drying and calcining in air. This basic approach has many variables such as for example the  set between 7 and 9, since previous studies have shown that this value corresponds to the proper synthesis temperature and the pH. In general the pH is set between 7 and 9, since previous studies  compromise between the loading of high gold amount on the support and the achievement of particles have shown that this value corresponds to the proper compromise between the loading of high gold   [6]. with the proper size (1–10 nm) [6]. amount on the support and the achievement of particles with the proper size (1–10 nm) The colloidal methods involve the use of a surfactant, which has the fundamental role of limiting The colloidal methods involve the use of a surfactant, which has the fundamental role of limiting  the growth and aggregation of the particles, forming a monolayer on the surface of the metal particles; the growth and aggregation of the particles, forming a monolayer on the surface of the metal particles;  inin this way also the long‐term stability of the catalyst increases. Such a stage of formation of micelles  this way also the long-term stability of the catalyst increases. Such a stage of formation of micelles by 3+ to Au03+ the agentagent  is followed by a reduction stage from Aufrom  the first colloidal by protective the  protective  is  followed  by  a  reduction  stage  Au .  Historically, to  Au0.  Historically,  the  first  method involves the reduction of gold ions by citrate [7]. Methods that are much more efficient usemore  the colloidal  method  involves  the  reduction  of  gold  ions  by  citrate  [7].  Methods  that  are  much  NaOH/tetrakis (hydroxymethyl) phosphonium chloride (THPC) system or organic polymers using efficient use the NaOH/tetrakis (hydroxymethyl) phosphonium chloride (THPC) system or organic  NaBH as a reducing agent. In the first case the NaOH/THPC system protects the gold particles, to polymers using NaBH 4 as a reducing agent. In the first case the NaOH/THPC system protects the  4 prevent aggregation, and it also works as in situ generator of the reducing agent [8]. In the second case, gold particles, to prevent aggregation, and it also works as in situ generator of the reducing agent [8].  polymers such as polyvinyl alcohol (PVA) or polyvinylpyrrolidone (PVP) may be used as stabilizing In the second case, polymers such as polyvinyl alcohol (PVA) or polyvinylpyrrolidone (PVP) may be  agents. They are commercially available, relatively inexpensive, soluble in water, non-toxic and both used as stabilizing agents. They are commercially available, relatively inexpensive, soluble in water,  have a long chain of carbon atoms functionalized, respectively, with OH groups and pyrrolidone non‐toxic and both have a long chain of carbon atoms functionalized, respectively, with OH groups  groups, as reported in Scheme 1. and pyrrolidone groups, as reported in Scheme 1. 

(a)

(b)

Scheme 1. (a) polyvinyl alcohol (PVA) and (b) polyvinylpyrrolidone (PVP).  Scheme 1. (a) polyvinyl alcohol (PVA) and (b) polyvinylpyrrolidone (PVP).

As the choice of the support plays a vital role in determining the activity and selectivity of a  As the choice of the support plays a vital role in determining the activity and selectivity of a catalyst, in order to assess the effect of the support we have investigated three different metal oxides.  catalyst, in order to assess the effect2, as it is inexpensive and has high chemical stability; ZrO of the support we have investigated three different metal 2oxides. In particular, we have chosen TiO , for its  In particular, we have chosen TiO2 , as it is inexpensive and has high chemical stability; ZrO2 , for its thermal and chemical stability; CeO 2, because it has a high ability to store and deliver oxygen (this is  thermal and chemical stability; CeO2 , because it has a high ability to store and deliver oxygen (this is a a very important point for oxidation reactions), due to its ability to change the oxidation state from  very important point for oxidation reactions), due to its ability to change the oxidation state from +3 to +3 to +4 [9]. Moreover, essential characteristics for a support are its stability in the reaction media, a  +4 [9]. Moreover, essential characteristics for a support are its stability in the reaction media, a low cost, low cost, adequate porous structure, acid‐base properties [10] and high surface area. In fact, a good  adequate porous structure, acid-base properties [10] and high surface area. In fact, a good support support must be able to allow a high dispersion of the active phase with a high degree of thermal  must be able to allow a high dispersion of the active phase with a high degree of thermal stability of stability of the catalyst.  the catalyst. The  aim  of  the  work  is  the  synthesis  of  new  gold  catalysts  active  in  oxidation  reactions.  As  The ofwe  the have  work investigated  is the synthesis of oxidation  new gold catalysts active in oxidation reactions. As catalytic catalytic aim test  the  of  glucose,  which  is  a  product  derivable  from  test we have investigated the the  oxidation glucose, which a product derivable processes  from biomass and biomass  and  exploited  for  future ofbio‐refinery.  The isbiomass  conversion  must  be  exploited for the future bio-refinery. The biomass conversion processes must be competitive from competitive from an economic point of view but must be also environmentally sustainable. Therefore,  an economic of view but must betechnologies.  also environmentally sustainable. wefraction  need theof  we  need  the point development  of  specific  Particular  attention  is Therefore, paid  to  the  development of specific technologies. Particular attention is paid to the fraction of lignocellulosic lignocellulosic  biomass  (LCF)  that  is  the  indigestible  part  of  plants; in  fact, it is  not  only  the  most  biomass (LCF) that isin the indigestible part of plants; made  in fact,up  it isof  not only the most abundant fraction in abundant fraction  nature,  but  it  is  completely  carbohydrates, in  particular glucose,  nature, but it is completely made up of carbohydrates, in particular glucose, which are a rich source of which are a rich source of carbon. In fact, they can be then converted into a set of “bio‐platform”  molecules (BPM) which can be used as starting materials for the production of high commercial value 

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carbon. In fact, they can be then converted into a set of “bio-platform” molecules (BPM) which can be used as starting materials for the production of high commercial value products. In the literature there are few studies on the oxidation of glucose to glucaric acid [11], while great interest is placed at the oxidation to gluconic acid, because of its wide field of industrial applications (the annual world production of gluconic acid is of 100,000 tonnes [12]). Currently, at industrial level, the gluconic acid is produced by enzymatic oxidation of glucose, using biotechnological processes involving bacteria such as Aspergillus niger and Gluconobacter suboxydans [13]. However, this process presents considerable disadvantages, both from the practical point of view and from the economic point of view, as the removal of waste water; the low rate of reaction, the death of the bacteria and the accumulation of the substances secreted by these [14]. The existence of such problems using enzymatic oxidation for the production of gluconic acid, has led the scientific community to look for an alternative view by green chemistry. A sustainable method is the oxidation with oxygen in mild reaction conditions of an aqueous glucose solution using a heterogeneous catalyst. The first reaction of carbohydrate oxidation mediated by catalysts was conducted in 1861 by von Gorup-Besanez [15], using a Pt-based catalyst. In the last two decades studies have been conducted on the use of catalysts based on Pt and Pd for the oxidation reaction of D-glucose to gluconic acid, which, however, have the disadvantages of not being selective against oxidation of the group aldehyde [16] and undergo deactivation in basic and acid environments. This last point is crucial: the rate of the oxidation reaction of a carbohydrate mainly depends on the metal and on the pH, while the selectivity depends primarily on pH and temperature [17]. The dependence of the reaction by the pH rate is determined by the fact that in basic conditions, the carbohydrate is present in the open linear form, in which it is present an aldehyde group, which is much more reactive towards oxidation than hydroxyl. The selectivity problems can be overcome by promoting catalysis, by the Pt or Pd [18], with metals such as Bi or Pb, but these catalysts are not stable since they suffer from leaching. After the discovery of the ability of gold nanoparticles to catalyze oxidation reactions, the interest in gold as a catalyst in selective oxidation of carbohydrates increased. In fact, gold is more resistant to deactivation than Pt and Pd. Moreover, due to this characteristic, the use of gold-based catalysts takes the advantage of being able to work in a wide range of pH, which is not possible with the biotechnological process. In 2002, Biella et al. [19] published studies on glucose oxidation reaction using Au/C catalyst and obtaining a selectivity of 100% with respect to gluconic acid. An effect of the support for the reaction was found by Ishida et al. [20] comparing catalysts supported on metal oxides (TiO2 , CeO2 , ZrO2 and Al2 O3 ) and one supported on C. The stability of the catalyst was proven by Baatz et al. [21] who recycled 20 times at 40 ˝ C an Au/Al2 O3 catalyst and from Mirescu et al. [22] who recycled 17 times at 40 ˝ C an Au/TiO2 catalyst. In the present paper it was investigated the oxidation of glucose. The aim of the work is the synthesis of new gold catalysts active in oxidation reactions. The catalysts must have very small gold particles and must be well dispersed on the support. For this reason, we have compared the two synthetic approaches of deposition precipitation (DP) and colloidal methods. It was also rated as the different surfactants used in the colloidal method affect the behavior of the catalyst. 2. Results and Discussion 2.1. Preliminary Characterizations N2 physisorption analyses were carried out in order to determine surface areas and pore size distributions of the supports. The supports exhibited isotherms with hysteresis loops typical of mesoporous materials (Figure 1) and had unimodal pore size distribution. The adsorption for zirconia support is shifted towards higher values, indicating the presence of larger pores (21 nm) than ceria and titania (5 nm and 4 nm). Zirconia exhibited the lowest surface area («40 m2 /g), while ceria and titania exhibited surface area values of 105 m2 /g and 166 m2 /g respectively.

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  Figure 1. Adsoption—desorption isotherms for the three supports.  Figure 1. Adsoption—desorption isotherms for the three supports.

All the catalysts were prepared with a nominal value of 2 wt. % Au. The analyses by atomic  All the catalysts were prepared with a nominal value of 2 wt. % Au. The analyses by atomic absorption (Table 1) show that for the samples prepared by colloidal method with a neutral surfactant,  absorption (Table 1) show that for the samples prepared by colloidal method with a neutral surfactant, such as PVA and PVP, it is possible to load all the theoretical metal; only ATiPVP is an exception. On  such as PVA and PVP, it is possible to load all the theoretical metal; only ATiPVP is an exception. the  contrary,  use ofof  THPC  as  surfactant,  obtaining  On the contrary,the  the use THPC as surfactant, allowsallows  obtaining catalystscatalysts  with onlywith  the 1 only  wt. %the  of   1 wt. % of the metal. As regard as DP technique, it allows synthesizing catalysts with a gold content  the metal. As regard as DP technique, it allows synthesizing catalysts with a gold content between between 1.5–1.8 wt. %.  1.5–1.8 wt. %. Table  reports also also the the amounts amounts ofof SO SO242− ion  chromatography, chromatography, that that are are  ´  ions,  Table 11 reports ions, determined  determined by  by ion 4 present in the final catalysts. In fact during some synthetic procedures, the support was acidified at  present in the final catalysts. In fact during some synthetic procedures, the support was acidified at pH = 1 with H SO4. In particular, it is possible to observe that for the samples supported on TiO 2 the  pH = 1 with H22SO 4 . In particular, it is possible to observe that for the samples supported on TiO2 the quantity of sulphate ions is remarkable. This is reasonable, since for this support in order to load an  quantity of sulphate ions is remarkable. This is reasonable, since for this support in order to load an adequate gold amount it is necessary to acidify the support before metal introduction [23].  adequate gold amount it is necessary to acidify the support before metal introduction [23]. Table 1. Metal and sulphates content by AAS and IC.  Table 1. Metal and sulphates content by AAS and IC.

Au Content SO42− Content Au Content SO4 2´ Content (wt. %)  (wt. %)  Sample (wt. %) (wt. %) ACeDP300  1.7    ACeDP300 1.7 AZrDP300  1.5    AZrDP300 1.5 ATiDP300  1.8    ATiDP300 1.8 ACePVA  1.8  0  ACePVA 1.8 0 AZrPVA 1.9 0 AZrPVA  1.9  0  ATiPVA 1.8 0.59 ATiPVA  1.8  0.59  ACePVP 1.8 0 ACePVP  1.8  0  AZrPVP 1.9 0 AZrPVP  1.9  0  ATiPVP 1.4 ACeTHPC 1.0 0 ATiPVP  1.4    AZrTHPC 0.9 0.8 ACeTHPC  1.0  0  ATiTHPC 1.0 1.0 AZrTHPC  0.9  0.8  ATiTHPC  1.0  1.0  As previously reported, in the sol immobilization method the surfactant has the fundamental role of maintaining gold nanoparticles separated, in order to avoid their sintering and deactivation. As previously reported, in the sol immobilization method the surfactant has the fundamental  With the aim of check for surfactant presence in the final samples, we have performed FT-IR analyses role of maintaining gold nanoparticles separated, in order to avoid their sintering and deactivation.  (Figure 2). In the range between 2950–2850 cm´1 , it’s possible to observe as for PVA and THPC series With the aim of check for surfactant presence in the final samples, we have performed FT‐IR analyses  some bands that are associated to the stretching−1of C–H aldehydic groups. This is a clear indication of (Figure 2). In the range between 2950–2850 cm , it’s possible to observe as for PVA and THPC series  the presence of surfactant in the final catalysts. some bands that are associated to the stretching of C–H aldehydic groups. This is a clear indication  of the presence of surfactant in the final catalysts.  Sample 

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Figure 2.Figure 2. FT‐IR spectra for catalysts synthesized by sol immobilization method with PVA (section a)  FT-IR spectra for catalysts synthesized by sol immobilization method with PVA (section a)   and THPC (section b).  and THPC (section b). Figure 2. FT‐IR spectra for catalysts synthesized by sol immobilization method with PVA (section a)  2.2. Effect of Support and of Synthetic Method 

2.2. Effect and THPC (section b).  of Support and of Synthetic Method

Figure 3 shows catalysts’ activity for  D‐glucose oxidation, which was used as reaction test. In 

Figure 3 shows catalysts’ activityeffect,  for D-glucose oxidation, was by  used as reaction test.To In order 2.2. Effect of Support and of Synthetic Method  order  to  highlight  the  support’s  all  the  catalysts  were which assembled  synthesis  method.  monitor  the  conversion  of  D ‐glucose  to  gluconic  acid,  the  latter  was  titrated  with  NaOH,  to highlight the support’s effect, all the catalysts were assembled by synthesis method. while  To monitor Figure 3 shows catalysts’ activity for  D‐glucose oxidation, which was used as reaction test. In  keeping the pH constant at 9.5 during the reaction. All the results obtained with the titration were  the conversion of D -glucose to gluconic acid, the latter was titrated with NaOH, while keeping order  to  highlight  the  support’s  effect,  all  the  catalysts  were  assembled  by  synthesis  method.  To  the compared  with  those  obtained  by  means  of  ion  chromatography.  In  all  cases  IC  results  were  pH constant at 9.5conversion  during theof reaction. All resultsacid,  obtained withwas  thetitrated  titration were compared monitor  the  D‐glucose  to the gluconic  the  latter  with  NaOH,  while with comparable with those of titration. The moles of initial glucose are exactly the same moles of final  thosekeeping the pH constant at 9.5 during the reaction. All the results obtained with the titration were  obtained by means of ion chromatography. In all cases IC results were comparable with those of product  when  conversion  is  complete.  At  the  same  time,  no  other  byproduct  have  been  detected.  compared  with  those  obtained  by  are means  of  ion  In  all product cases  IC when results  were  titration. The moles of initial glucose exactly thechromatography.  same moles of final conversion Regardless of the synthetic methodology, the catalysts supported on ceria are those that show the  comparable with those of titration. The moles of initial glucose are exactly the same moles of final  is complete. At the same time, no other byproduct have been detected. Regardless of the synthetic highest activity. On the contrary, those supported on titania present the lowest conversion. Moreover,  product  when  conversion  is  complete.  At  the  time,  other  byproduct  have  been  the effect of the support seems to have little influence on the catalysts prepared by PVP as surfactant:  methodology, the catalysts supported on ceria aresame  those thatno  show the highest activity. Ondetected.  the contrary, Regardless of the synthetic methodology, the catalysts supported on ceria are those that show the  in this case, all as prepared samples show limited activity.  those supported on titania present the lowest conversion. Moreover, the effect of the support seems highest activity. On the contrary, those supported on titania present the lowest conversion. Moreover,  to have little influence on the catalysts prepared by PVP as surfactant: in this case, all as prepared the effect of the support seems to have little influence on the catalysts prepared by PVP as surfactant:  samples show limited activity. in this case, all as prepared samples show limited activity. 

 

 

 

 

Figure 3. Conversions vs. time of reaction for D-glucose oxidation at 60 ˝ C and 100 cc/min of O2 for samples synthesized by DP, PVA, PVP and THPC.

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Figure 3. Conversions vs. time of reaction for  D‐glucose oxidation at 60 °C and 100 cc/min of O2 for  samples synthesized by DP, PVA, PVP and THPC. 

As regard as catalysts prepared using PVA as surfactant, the CeO2 supported sample is very active unlike those supported on ZrO2 and TiO2 ; the latter present very2 supported sample is very  similar profiles and there As regard as catalysts prepared using PVA as surfactant, the CeO seems to be no effectsupported  of the support. To2 and  test whether the low present  activity very  depends on the size ofand there  the gold active unlike those  on  ZrO TiO2;  the  latter  similar  profiles  particles, the samples were analyzed by SEM and TEM. seems to be no effect of the support. To test whether the low activity depends on the size of the gold  Figure 4 reports EDX-SEM analyses, in particular the elemental mapping for ACePVA (section a), particles, the samples were analyzed by SEM and TEM.  AZrPVA (section b) and ATiPVA (section c) samples. Both ACePVA and AZrPVA present almost all Figure 4 reports EDX‐SEM analyses, in particular the elemental mapping for ACePVA (section  gold particles smaller than 10 nm. On the contrary, sample supported on titania present some big a), AZrPVA (section b) and ATiPVA (section c) samples. Both ACePVA and AZrPVA present almost  agglomerates and very big gold particles. all gold particles smaller than 10 nm. On the contrary, sample supported on titania present some big  agglomerates and very big gold particles. 

a

b

c

  Figure 4. EDX elemental mapping of samples ACePVA (section a), AuZrPVA (section b) and ATiPVA  Figure 4. EDX elemental mapping of samples ACePVA (section a), AuZrPVA (section b) and ATiPVA (section c).  (section c).

In  Figure  5  there  are  shown,  respectively  for  the  CeO2  (section  a),  ZrO2  (section  b)  and  TiO2  In Figure 5 there are shown, respectively for the CeO2 (section a), ZrO2 (section b) and TiO2 (section c) samples, TEM images. TEM data agree with SEM results. The average particle diameter of  (section c) samples, TEM images. TEM data agree with SEM results. The average particle diameter of ACePVA is 3.5 nm, while that of AZrPVA is 4.8 nm. The sample supported on CeO2 shows a particle  ACePVA is 3.5 nm, while that of AZrPVA is 4.8 nm. The sample supported on CeO2 shows a particle size  distribution  narrower  than  that  based  on  ZrO2.  Therefore,  both  samples  have  particles  small  size distribution narrower than that based on ZrO2 . Therefore, both samples have particles small enough to be able to catalyze the glucose oxidation. As regard as ATiPVA, the TEM images, shows  enough to be able to catalyze the glucose oxidation. As regard as ATiPVA, the TEM images, shows the the presence of some particles with small size (5–7 nm) but also of many particles whose diameter is  presence of some particles with small size (5–7 nm) but also of many particles whose diameter is much much larger than those of the two other catalysts prepared in the same way.  larger than those of the two other catalysts prepared in the same way.

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Figure 5. TEM images for ACePVA (section a); AZrPVA (section b) and ATiPVA (section c). Figure 5. TEM images for ACePVA (section a); AZrPVA (section b) and ATiPVA (section c). 

Also as regard as samples prepared by THPC, the catalyst supported on ceria is the most active. Also as regard as samples prepared by THPC, the catalyst supported on ceria is the most active.  To determine whether the poor activity of the other samples depends on the size of the particles the To determine whether the poor activity of the other samples depends on the size of the particles the  catalysts were analyzed by SEM and TEM microscopy. From these analyses (Figure 6) it’s evident that catalysts were analyzed by SEM and TEM microscopy. From these analyses (Figure 6) it’s evident  the particle size does depend only on the on  synthesis methodology but alsobut  on also  the kind of support. that  the  particle  size  not does not  depend  only  the  synthesis  methodology  on  the  kind  of  In fact for the sample supported on ceria it is possible to obtain gold nanoparticles of about 4 nm, and support. In fact for the sample supported on ceria it is possible to obtain gold nanoparticles of about  the sample active in the oxidation reaction of glucose. As for the catalyst oncatalyst  zirconiabased  it shows 4  nm,  and  is the  sample  is  active  in  the  oxidation  reaction  of  glucose.  As based for  the  on  a particle size only slightly larger than that supported on ceria. For this reason, by TEM analysis it’s zirconia it shows a particle size only slightly larger than that supported on ceria. For this reason, by  impossible to understand why it is inactive. Conversely, the catalyst supported on titania is inactive TEM analysis it’s impossible to understand why it is inactive. Conversely, the catalyst supported on  due to some metallic agglomerates (Figure 6 section c). titania is inactive due to some metallic agglomerates (Figure 6 section c). 

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  Figure 6. Double SEM images obtained by two detector (AsB and Inlens) and TEM: ACeTHPC (section  Figure 6. Double SEM images obtained by two detector (AsB and Inlens) and TEM: ACeTHPC a); AZrTHPC (section b) and ATiTHPC (section c).  (section a); AZrTHPC (section b) and ATiTHPC (section c).

In order to explain the reason for the inactivity of some samples with a proper gold size, we have  In order to explain the reason for the inactivity of some samples with a proper gold size, we have assumed that an excess of surfactant could cover the surface of the catalyst, and affect its activity. So,  assumed that an excess of surfactant could cover the surface of the catalyst, and affect its activity. So, the the  samples  were  subjected  to  temperature  programmed  oxidation  (TPO)  in  order  to  assess  the  samples were subjected to temperature programmed oxidation (TPO) in order to assess the temperature temperature  of  oxidation  for  the  organic  component.  Figure  7  reports  the  trend  for  m/e  =  44,  of oxidation for the organic component. Figure 7 reports the trend for m/e = 44, corresponding to corresponding  to  the  CO2  mass,  which  is  formed  from  PVA  (section  a),  THPC  (section  b)  or  PVP  the CO2 mass, which is formed from PVA (section a), THPC (section b) or PVP (section c) oxidation. (section c) oxidation. For all the three systems, the TPO of ceria supported samples present peak at  For all the three systems, the TPO of ceria supported samples present peak at the lowest temperature. the  lowest  temperature.  For  the  AZrPVA  sample  two  wide  peaks  between  200  and  530  °C  were  For the AZrPVA sample two wide peaks between 200 and 530 ˝ C were observed. The TPO analysis observed. The TPO analysis of ATiPVA sample shows three very intense peaks, indicating that the  of ATiPVA sample shows three very intense peaks, indicating that the organic part of the surfactant organic part of the surfactant is oxidized between 300 and 600 °C. As regard as samples prepared by  is oxidized between 300 and 600 ˝ C. As regard as samples prepared by THPC, the TPO analysis of THPC, the TPO analysis of the sample supported on ceria shows a single peak between 200 and 400  the sample supported on ceria shows a single peak between 200 and 400 ˝ C. Both AZrTHPC and °C. Both AZrTHPC and ATiTHPC present two peaks between 250 and 600 °C, at higher temperature  than ACeTHPC sample. Samples prepared using PVP as surfactant show low activity, regardless of 

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ATiTHPC present two peaks between 250 and 600 ˝ C, at higher temperature than ACeTHPC sample. Samples PVP as surfactant show lowprofiles  activity,are  regardless of in  theFigure  used support (Figure 3, the  used prepared support  using (Figure  3,  section  c).  Their  TPO  reported  7  (section  c).  As  Catalysts 2016, 6, 87  9 of 16  section c). Their TPO profiles are reported in Figure 7 (section c). As regard as ceria, the TPO shows a regard as ceria, the TPO shows a single peak between 200 and 300 °C. The TPO analysis for AZrPVP  the  used  support  3, 300 section  c). 600  Their  TPO  profiles  are  in  Figure  7  (section  c). in  As  single peak between 200 and The TPO analysis for AZrPVP shows three three  peaks between 300 shows  three  peaks (Figure  between  300 ˝ C. and  °C,  while  that  for reported  ATiPVP  shows  peaks  the  ˝ C, while that for ATiPVP shows three peaks in the temperature range 400–550 ˝ C. regard as ceria, the TPO shows a single peak between 200 and 300 °C. The TPO analysis for AZrPVP  and 600 temperature range 400–550 °C.  shows  three  peaks  between  300  and  600  °C,  while  that  for  ATiPVP  shows  three  peaks  in  the  temperature range 400–550 °C. 

(a) 

(b)

(c) 

(a)  (b) (c)  Figure 7. TPO analysis (m/e = 44) for samples synthesized by PVA (section a), THPC (section b), PVP  Figure 7. TPO analysis (m/e = 44) for samples synthesized by PVA (section a), THPC (section b), PVP (section c).  Figure 7. TPO analysis (m/e = 44) for samples synthesized by PVA (section a), THPC (section b), PVP  (section c). (section c). 

Therefore, the inactive samples were calcined at proper temperatures in order to remove part or  Therefore, the inactive samples were calcined at proper temperatures in order to remove part Therefore, the inactive samples were calcined at proper temperatures in order to remove part or  almost all the surfactant. Calcined catalysts were then tested in the glucose oxidation (Figure 8). As  or almost all the surfactant. Calcined catalysts were then tested in the glucose oxidation (Figure 8). As  almost all the surfactant. Calcined catalysts were then tested in the glucose oxidation (Figure 8). regard as PVA series, both the samples calcined at 300 °C, where only part of PVA was removed, are  Asregard as PVA series, both the samples calcined at 300 °C, where only part of PVA was removed, are  regard as PVA series, both the samples calcined at 300 ˝ C, where only part of PVA was removed, much more active than the corresponding non‐calcined samples. On the contrary, a drop in activity  aremuch more active than the corresponding non‐calcined samples. On the contrary, a drop in activity  much more active than the corresponding non-calcined samples. On the contrary, a drop in activity was observed after calcination at 500 °C of the AZrPVA and therefore after removal of almost all PVA.  was observed after calcination at 500 ˝ C of the AZrPVA and therefore after removal of almost all PVA. was observed after calcination at 500 °C of the AZrPVA and therefore after removal of almost all PVA.  As regard as ATiPVA sample calcination at 500 °C does not lead to a total collapse of the activity. In  AsAs regard as ATiPVA sample calcination at 500 °C does not lead to a total collapse of the activity. In  regard as ATiPVA sample calcination at 500 ˝ C does not lead to a total collapse of the activity. In fact, fact, at that temperature the surfactant is still partially present, as evidenced by TPO analysis. The  at that temperature the surfactant is still partially present, as evidenced by TPO analysis. The surfactant fact, at that temperature the surfactant is still partially present, as evidenced by TPO analysis. The  surfactant  has  the  fundamental  role  of  keeping  separate  the  gold  particles  and  preventing  the  hassurfactant  the fundamental of keeping role  separate the goldseparate  particlesthe  andgold  preventing theand  sintering. Whenthe  PVA has  the role fundamental  of  keeping  particles  preventing  sintering. When PVA is totally eliminated the particles coagulate and are no more able of activate  sintering. When PVA is totally eliminated the particles coagulate and are no more able of activate  is totally eliminated the particles coagulate and are no more able of activate oxygen. oxygen.  oxygen.  100 100

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Figure 8. Conversions vs. time of reaction for DD-glucose oxidation at 60 and 100 cc/min of 2O Figure 8. Conversions vs. time of reaction for  ‐glucose oxidation at 60 °C and 100 cc/min of O  for  2 for Figure 8. Conversions vs. time of reaction for  D‐glucose oxidation at 60 °C and 100 cc/min of O2 for  calcined samples. calcined samples.  calcined samples. 

 

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As regard as THPC samples, to estimate changes in the activity of the catalysts by removing part Catalysts 2016, 6, x  10 of 16  or all the surfactant, the samples were calcined at 300 and 500 ˝ C and then tested in the D-glucose oxidation. Even after calcination the AZrTHPC sample is inactive, indicating that the gold particles oxidation. Even after calcination the AZrTHPC sample is inactive, indicating that the gold particles  are in a size range too high to catalyze the reaction. In fact, as can be seen from the analysis TEM and are in a size range too high to catalyze the reaction. In fact, as can be seen from the analysis TEM and  SEM, they have a diameter twice than that of particles in the AZrPVA sample. SEM, they have a diameter twice than that of particles in the AZrPVA sample.  Taking account thethe  TPO analyses the samples ACePVP, ATiPVP and AZrPVP were calcined, Taking into into  account  TPO  analyses  the  samples  ACePVP,  ATiPVP  and  AZrPVP  were  ˝ C. As evidenced by the concentration profiles vs. time (Figure 8), respectively, at 200, 300 and 400 calcined,  respectively,  at  200,  300  and  400  °C.  As  evidenced  by  the  concentration  profiles  vs.  time  in(Figure  the case samples on ceria and zirconia calcination leads to an improvement in 8), ofin the the  case  of supported the  samples  supported  on  ceria  and  zirconia  calcination  leads  to  an  the activity. On the contrary, as regard the sample ATiPVP calcination does not lead to any change improvement in the activity. On the contrary, as regard the sample ATiPVP calcination does not lead  into any change in its activity. Therefore, it is assumed that in this case the poor performance does not  its activity. Therefore, it is assumed that in this case the poor performance does not depend on an excessive amount of surfactant, but on the too large size of gold particles. depend on an excessive amount of surfactant, but on the too large size of gold particles.  The AZrPVA300 and AZrPVA500 catalysts were were  analyzed by FT-IR (Bruker Tensor 27, The  AZrPVA300  and  AZrPVA500  catalysts  analyzed  by spectroscopy FT‐IR  spectroscopy  (Bruker  Bruker Optik GmbH, Ettlingen, Germany) to confirm the disappearance of the PVA during calcination Tensor 27, Bruker Optik GmbH, Ettlingen, Germany) to confirm the disappearance of the PVA during  (Figure 9, section a). In the spectra of AZrPVA there are evidences of the peaks associated with calcination (Figure 9, section a). In the spectra of AZrPVA there are evidences of the peaks associated  the stretching vibration of the CH Their intensity the calcination with  the  stretching  vibration  of bond. the  CH  bond.  Their decreases intensity with decreases  with  the temperature, calcination  evidencing the gradual surfactant elimination. temperature, evidencing the gradual surfactant elimination.  TEM in order  order to  to observe  observe how  how the  the nanoparticles  nanoparticlessize  size TEM and and SEM SEM analysis analysis were were also also performed performed  in  changes by varying the calcination temperature (Figure 9, section b). In the sample calcined at 300 ˝ C changes by varying the calcination temperature (Figure 9, section b). In the sample calcined at 300 °C  the mean mean particle particle size size isis almost almost the the same same of of the the uncalcined uncalcined  sample. sample.  On  the On the  thecontrary,  contrary, the  thesample  sample calcined at 500 °C shows agglomerates (Figure 9, section c). This is an indication of the collapse of the  calcined at 500 ˝ C shows agglomerates (Figure 9, section c). This is an indication of the collapse of the gold nanoparticles, due to the almost complete removal of the surfactant.  gold nanoparticles, due to the almost complete removal of the surfactant.  

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Figure 9. FT-IR spectra of AZrPVA (thick), AZrPVA300 (thin); AZrPVA500 (dashed) (section a). Figure 9. FT‐IR spectra of AZrPVA (thick), AZrPVA300 (thin); AZrPVA500 (dashed) (section a). TEM  TEM images of the AZrPVA300 (section b) and AZrPVA500 samples (section c). images of the AZrPVA300 (section b) and AZrPVA500 samples (section c). 

From this investigation it is evident that ceria is the best support, both for its ability to allow the  synthesis of gold nanoparticles small enough to be active, both for its ability to function as oxygen 

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From this investigation it is evident that ceria is the best support, both for its ability to allow the synthesis of gold nanoparticles small enough to be active, both for its ability to function as oxygen Catalysts 2016, 6, 87  11 of 16 of all pump. The latter is fundamental when using oxygen as reagent. Table 2 reports conversions catalysts in order to determine the effect of synthetic methodology on the catalytic activity. pump. The latter is fundamental when using oxygen as reagent. Table 2 reports conversions of all  By comparing the various synthetic techniques, it is clear that, regardless of the support, the catalysts in order to determine the effect of synthetic methodology on the catalytic activity.  ˝ C, is the one that allows obtaining method using the colloidal PVA, followed by calcination at 300that,  By  comparing  the  various  synthetic  techniques,  it  is  clear  regardless  of  the  support,  the  samples with the best performance. This is a confirmation that a surfactant with a steric effect such as method using the colloidal PVA, followed by calcination at 300 °C, is the one that allows obtaining  PVA samples with the best performance. This is a confirmation that a surfactant with a steric effect such  acts as a better protective agent with respect nanoparticles, compared to THPC, which acts by electrostatic effect. as PVA acts as a better protective agent with respect nanoparticles, compared to THPC, which acts  by electrostatic effect.  Table 2. Comparison of conversions after 60 min of reaction for D-glucose oxidation at 60 ˝ C and 100 cc/min of O2 . Table 2. Comparison of conversions after 60 min of reaction for  D‐glucose oxidation at 60 °C and 100  cc/min of O2. 

Conv. Conv. (%) (%)  67 67 52 52 28 28  79 79  73 73 54 54 68 68 2 2 10 10 43 43 32 32 4 4 

Sample Sample  ACeDP300 ACeDP300  AZrDP300 AZrDP300  ATiDP300 ATiDP300  ACePVA300 ACePVA300  AZrPVA300 AZrPVA300  ATiPVA300 ATiPVA300  ACeTHPC ACeTHPC  AZrTHPC AZrTHPC  ATiTHPC ACePVP200 ATiTHPC  AZrPVP300 ACePVP200  ATiPVP400 AZrPVP300  ATiPVP400 

2.3. Investigation on Catalysts’ Recycle 2.3. Investigation on Catalysts’ Recycle 

To complete the work, we studied the recycling of the best performing catalyst, i.e., ACePVA. To complete the work, we studied the recycling of the best performing catalyst, i.e., ACePVA.  At the end of the reaction, the catalyst was filtered, washed with distilled water, dried and subsequently At  the  end  of  the  reaction,  the  catalyst  was  filtered,  washed  with  distilled  water,  dried  and  reused. In Figure 10 there are shown the conversion profiles vs. time of the fresh and reused samples. subsequently reused. In Figure 10 there are shown the conversion profiles vs. time of the fresh and  The catalyst proves recyclable, without undergoing any treatment unless filtration, washing and drying. reused samples. The catalyst proves recyclable, without undergoing any treatment unless filtration,  In fact, it shows loss of after each reuse, indicating is notindicating  subject to phenomena washing  and no drying.  In activity fact,  it  shows  no  loss  of  activity  after  that each it reuse,  that  it  is  not  of leaching, sintering or coating of the surface. subject to phenomena of leaching, sintering or coating of the surface.  100 90

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3. Materials and Methods 

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3. Materials and Methods 3.1. Materials (NH4 )2 Ce(NO3 )6 (ě98.5%, Sigma-Aldrich, St Louis, MO, USA); ZrOCl2 ¨ 8H2 O (ě99.5%, Sigma-Aldrich, Sigma Aldrich Chemie, GMBH, Riedstr, Germany); TiOSO4 ¨ H2 SO4 ¨ H2 O (Aldrich Chemistry, Sigma-Aldrich, St Louis, MO, USA); PVA (98% P.M. 13.000–23.000 d, Sigma-Aldrich); PVP (P.M. 40.000 d, Sigma-Aldrich); THPC (80% in water, Aldrich Chemistry). 3.2. Synthesis of Supports Ceria support was synthesized by precipitation from (NH4 )2 Ce(NO3 )6 by urea at 100 ˝ C in aqueous solution [24,25]. The solution was mixed and boiled for 6 h at 100 ˝ C, the precipitate was washed twice in boiling deionized water and dried at 110 ˝ C overnight. The material was then calcined in flowing air (50 mL/min) at 500 ˝ C for 3 h. Zr(OH)4 was prepared by precipitation from ZrOCl2 ¨ 8H2 O at constant pH = 8.6 and then aged for 20 h at 90 ˝ C [26,27]. Then zirconium hydroxide was calcined in air (30 mL/min STP) at 650 ˝ C for 3 h. Titanium hydroxide was precipitated at pH = 8.6 from 0.5 M titanyl sulphate aqueous solution [28,29]. In particular, 40 g of TiOSO4 xH2 SO4 yH2 O (Aldrich) were dissolved in 300 mL of distilled water at room temperature under vigorous stirring. The Ti(OH)4 precipitation was obtained by the drop wise addition of 9 M ammonia solution under vigorous stirring. The suspension was magnetically stirred at 60 ˝ C for 20 h. Then the precipitate was filtered, washed with distilled water in order to remove SO4 2´ ions and dried at 110 ˝ C for 18 h. The absence of sulphates in the material was verified by IEC analysis. Finally the hydroxide was calcined in air flow at 300 ˝ C for 3 h. 3.3. Synthesis of Catalysts In all cases the amount of support was calculated as having a final gold loading of 2 wt. %. 3.3.1. Synthesis by Deposition Precipitation (DP) Gold was added by DP method at pH = 8.6. The oxide supports were suspended in an aqueous solution of HAuCl4 ¨ 3H2 O for 3 h and the pH was controlled by the addition of NaOH (0.5 M). After filtration the samples were dried at 35 ˝ C overnight and finally calcined in air for 1 h at 300 ˝ C. Samples where denoted as ACeDP300, AZrDP300 and ATiDP300. 3.3.2. Synthesis by PVA Sol-Imobilization A 1 wt. % polyvinyl alcohol (PVA) solution was added to an aqueous HAuCl4 solution under vigorous stirring at 0 ˝ C (Au/PVA (w/w) = 2) [30]. Then a 0.1 M of freshly prepared solution of NaBH4 (NaBH4 /Au (mol/mol) = 4) was added, to form a ruby-red metallic sol. Within a few minutes of sol generation, the sol was immobilized by adding the support (CeO2 , ZrO2 or TiO2 . As regard as TiO2 , this has been previously acidified with H2 SO4 at pH = 1 [20]) under vigorous stirring and aged for 12 h. After filtration, the samples were washed for several times with distilled water. The samples were dried at 110 ˝ C overnight. Samples where denoted as ACePVA, AZrPVA and ATiPVA. Part of the final catalysts was finally calcined in air (30 mL/min) for 1 h at different temperatures. 3.3.3. Synthesis by PVP Sol-Imobilization A 2 wt. % polyvinylpyrrolidone (PVP) solution was added to an aqueous HAuCl4 solution under vigorous stirring at 0 ˝ C (Au/PVP (w/w) = 0.25) [31]. Then a 0.1 M of freshly prepared solution of NaBH4 (NaBH4 /Au (mol/mol) = 4) was added, to form a ruby-red metallic sol. Within a few minutes of sol generation, the sol was immobilized by adding the support (CeO2 , ZrO2 or TiO2 . As regard as TiO2 , this has been previously acidified with H2 SO4 at pH = 1) under vigorous stirring and aged for

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20 h. After filtration, the samples were washed for several times with distilled water. The samples were dried at 110 ˝ C overnight. Samples where denoted as ACePVP, AZrPVP and ATiPVP. Part of the final catalysts was finally calcined in air (30 mL/min) for 1 h at different temperatures. 3.3.4. Synthesis by THPC Sol-Imobilization A 0.05 M solution of tetrakis (hydroxymethyl)phosphonium chloride (THPC) was added to a M solution of NaOH (NaOH/THPC (mol/mol) = 0.042). Then, an aqueous HAuCl4 solution was added dropwise under vigorous stirring at room temperature (Au/THPC (w/w) = 1) [27]. The sol was immobilized by adding the support (CeO2 , ZrO2 or TiO2 previously acidified with H2 SO4 at pH = 1) under vigorous stirring and aged for 5 h. After filtration, the samples were washed for several times with distilled water. The samples were dried at 110 ˝ C overnight. Samples where denoted as ACeTHPC, AZrTHPC and ATiTHPC. Part of the final catalysts was finally calcined in air (30 mL/min) for 1 h at different temperatures.

10´3

3.4. Methods Surface areas and pore size distributions were obtained from N2 adsorption/desorption isotherms at ´196 ˝ C (using a Micromeritics ASAP 2000 analyser, Micromeritics, Milan, Italy). Surface area was calculated from the N2 adsorption isotherm by the BET equation, and pore size distribution was determined by the BJH method [32]. Total pore volume was taken at p/p0 = 0.99. The sulphate content was determined by ion chromatography (IC). Sulphate concentration was calculated as the average of two independent analyses, each including two chromatographic determinations. The gold amount for both fresh and exhausted catalysts was determined by atomic absorption spectroscopy (AAS) after microwave disaggregation of the samples (100 mg) using a Perkin-Elmer Analyst 100 (Perkin Elmer, Waltham, MA, USA). TEM images have been obtained using a Philips 208 Transmission Electron Microscope (FEI, Hillsboro, OR, USA). The samples were prepared by putting one drop of an ethanol dispersion of the catalysts on a copper grid pre-coated with a Formvar film and dried in air. SEM images have been obtained using a Field Emission Gun Electron Scanning Microscopy LEO 1525 (Carl Zeiss Microscopy, Jena, Germany), after metallization with graphite. The images were acquired by AsB (angular selective BSE) detector (Carl Zeiss Microscopy, Jena, Germany) while elemental composition was determined using Bruker Quantax EDS (Bruker Nano GmbH, Berlin, Germany). FTIR spectra were obtained on a Bruker spectrophotometer (Bruker Tensor 27, Bruker Optik GmbH, Ettlingen, Germany) (4 cm´1 resolution, MCT detector). Reduced samples were inspected in the form of self-supporting tablets (~25 mg¨ cm´2 ). Quartz cells (equipped with KBr windows) connected to a gas vacuum line equipped with rotary and turbomolecular pumps were used. Thermal analyses (TG/DTA) were performed on a STA 409 PC/PG instrument (NETZSCH, Selb, Germany) in flowing air (20 mL/min) with temperature rate set at 5 ˝ C/min in the 25–800 ˝ C temperature range. TPO measurements were carried out in a lab-made equipment: samples (100 mg) were heated with a temperature rate of 10 ˝ C/min from 25 ˝ C to 600 ˝ C in a 5% O2 /He flow (40 mL/min). The effluent gases were analyzed by a TCD detector (GOW-MAC Instrument Co., Shannon, Ireland) and by a Genesys 422 quadrupole mass analyzer (QMS) (EES Genesys House Northwich, Cheshire, UK). 3.5. Catalytic Reactions Commercial Dextrose (100% pure, PURE Dextro, distributed by Pro Nutrition) was used as substrate. Its oxidation to (Scheme 2) gluconic acid was carried out in a batch reactor, typically at atmospheric pressure and 60 ˝ C. In particular, 1 g of D-glucose in 50 mL of distilled H2 O was brought to pH = 9.5 by adding a solution of NaOH 0.1 M. An O2 flow (100 mL/min) was bubbled in the

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solution through a porous septum. Separately, 130 mg of catalyst were suspended in 20 mL of distilled Catalysts 2016, 6, 87  14 of 16  H 2 O, brought to pH = 9.5 with a solution 0.1 M of NaOH and then charged into the reactor. To monitor the conversion of D-glucose to gluconic acid, the latter was titrated with NaOH 0.1 M, while keeping conversion of  D‐glucose to gluconic acid, the latter was titrated with NaOH 0.1 M, while keeping the  the pH constant at 9.5 during the reaction. pH constant at 9.5 during the reaction.  H OH

OH O

O2

OH O HO H

HO H H

OH

H OH

Au catalyst 60°C pH 9.5

HO OH

OH

NaOH OH O

Na+

OH

O OH

OH

 

Scheme 2.2. Oxidation Oxidation  of  D‐glucose   to  gluconic  acid .  The  latter  is  to converted   to  sodium  Scheme of D -glucose to gluconic acid. The latter is converted sodium gluconate by gluconate by addition of NaOH.  addition of  NaOH.

The  results  obtained  with  the  titration  were  compared  with  those  obtained  by  means  of  ion  The results obtained with the titration were compared with those obtained by means of ion chromatography  (IC).  A  precolumn  Dionex  AG11,  a  column  Dionex  AS11,  a  suppressor  Dionex  chromatography (IC). A precolumn Dionex AG11, a column Dionex AS11, a suppressor Dionex ASRS‐300, a TCD Dionex ED40 (100 mA) were used. In all cases IC results were comparable with  ASRS-300, a TCD Dionex ED40 (100 mA) were used. In all cases IC results were comparable with those those of titration.  of titration. 4. Conclusions  4. Conclusions Heterogeneous catalysis plays a key role in the future biorefinery and there is great interest in  Heterogeneous catalysis plays a key role in the future biorefinery and there is great interest in the the  development  of  new  catalysts.  Focusing  on  the transformation  of  platform  through  development of new catalysts. Focusing on the transformation of platform moleculesmolecules  through oxidation oxidation reactions, the use of nanostructured catalysts based on gold seems to be the most promising  reactions, the use of nanostructured catalysts based on gold seems to be the most promising alternative. alternative. The work was focused on the synthesis of catalysts based on gold nanoparticles on three  The work was focused on the synthesis of catalysts based on gold nanoparticles on three different different supports (CeO 2, ZrO2 and TiO2) and two different techniques: deposition‐precipitation and  supports (CeO2 , ZrO2 and TiO2 ) and two different techniques: deposition-precipitation and colloidal colloidal method. Moreover, in the latter case the role of the surfactant (PVA, PVP, THPC) was also  method. Moreover, in the latter case the role of the surfactant (PVA, PVP, THPC) was also investigated. investigated. The catalysts were tested in a model reaction: the oxidation of glucose to gluconic acid,  The catalysts were tested in a model reaction: the oxidation of glucose to gluconic acid, which takes which takes place in an aqueous environment and under mild conditions (60 °C and atmospheric  place in an aqueous environment and under mild conditions (60 ˝ C and atmospheric pressure). pressure).  TEM and SEM analyses have shown as for catalysts prepared by colloidal method with PVA smallTEM and SEM analyses have shown as for catalysts prepared by colloidal method with PVA  gold nanoparticles are obtained. The latter is a necessary condition, but not sufficient for a good small gold nanoparticles are obtained. The latter is a necessary condition, but not sufficient for a good  conversion. In fact, for an active sample, the excess of surfactant must be removed because it would conversion. In fact, for an active sample, the excess of surfactant must be removed because it would  coat the surface of the catalyst. The surfactant, however, should not be completely eliminated, since it coat the surface of the catalyst. The surfactant, however, should not be completely eliminated, since  has the fundamental role of stabilizing the sample preventing nanoparticles from aggregation. it has the fundamental role of stabilizing the sample preventing nanoparticles from aggregation.  As for the catalysts prepared by colloidal method with THPC, only the sample supported on ceria As for the catalysts prepared by colloidal method with THPC, only the sample supported on  has sufficiently small nanoparticles. This is a clear indication that the size of the particles not only ceria has sufficiently small nanoparticles. This is a clear indication that the size of the particles not  depends on the synthetic procedure but also on the kind of support. only depends on the synthetic procedure but also on the kind of support.  As regard as catalysts prepared by DP, their activity is lower than samples prepared by PVA. As regard as catalysts prepared by DP, their activity is lower than samples prepared by PVA.  Moreover it is observed a minor effect of the support: catalysts prepared with DP technique do Moreover it is observed a minor effect of the support: catalysts prepared with DP technique do not  not show a big difference in activity. Samples prepared by colloidal method with PVP gave the show a big difference in activity. Samples prepared by colloidal method with PVP gave the worst  worst performance. performance.  It was evidenced that both the synthetic approach and the kind of support affect the catalysts’  activity. In fact, by focusing on the three different supports, with all the preparation methods, the  ceria has proved to be the best support. This is due to its ability to obtain small gold nanoparticles  and to its ability to accumulate oxygen. 

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It was evidenced that both the synthetic approach and the kind of support affect the catalysts’ activity. In fact, by focusing on the three different supports, with all the preparation methods, the ceria has proved to be the best support. This is due to its ability to obtain small gold nanoparticles and to its ability to accumulate oxygen. The most appropriate synthesis methodology proved to be the colloidal method with PVA. Combining the effect of the synthetic method and the effect of the support, ACePVA resulted the best performing catalyst. In fact, it is the most active and is completely recyclable after a simple washing with water. Acknowledgments: We thank G. Cruciani for TG/DTA measurements. technical assistance.

We thank Tania Fantinel for

Author Contributions: M.S. supervised the work; F.M. performed synthetic procedures, E.F. performed the catalytic experiments; A.D. performed TEM, FT-IR and SEM analyses. Conflicts of Interest: The authors declare no conflict of interest.

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