Electronic Supplementary Material (ESI) - Royal Society of Chemistry

Electronic Supplementary Material (ESI) for Molecular BioSystems. This journal is © The Royal Society of Chemistry 2014

Figure S1 Helical regions predicted by JPred in p53 TAD OrigSeq

: 1---------11--------21--------31--------41--------51-------- : : MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGP : OrigSeq

Jnet jhmm jpssm

: -----------------HHHHHHH---------------H--------HHHHHH------ : Jnet : ------------------HHHHH--------------HHH--------HHHHHH------ : jhmm : ----------------HHHHHHHHH--------------HHH-------HHHHH------ : jpssm

Jnet jhmm jpssm

- Final secondary structure prediction for query - Jnet hmm profile prediction - Jnet PSIBLAST pssm profile prediction

Electronic Supplementary Material (ESI) for Molecular BioSystems. This journal is © The Royal Society of Chemistry 2014

Electronic Supplementary Material (ESI) for Molecular BioSystems. This journal is © The Royal Society of Chemistry 2014

Figure S3

E2A family Leu 9aaTAD GTDK EL SD LL D FS MM GTDK EL SD LL D FS KM GTDK EL SD LL D LS MM ATDK EL SD LL D FS VM GTDK EL SD LL D FS AV GTDK DL SD LL D FS AM GTDM EL ND LL D FS IM ETDK EL ND LL D FT AM NQTK VL QE TL D FS LV SQTK IL QE TL D FS LV

FPLP FPLP FPLP FPLP RYP FSPP FPHP FELP TPTP TP

TFE2_HUMAN G1QMU8_NOMLE G3UGS9_LOXAF W5KGK3_ASTMX F7BGG6_XENTR F6Y2I2_CANFA Q90491_DANRE H2T6R8_TAKRU RTG3_YEAST J4TS14_SACK1

9aaTAD pattern of the E2A family: [SNQ][DE][LT]LD[FL][ST][MKVAIL][MV]

MLL family Leu GN LP GN LP GN LP GN IP ED LP ED LP SH IP SH IP SH IP

SD SD SD SD SE SE SE SE SE

9aaTAD IM D FV IM D FV IM E FV IM E FV IV D FV IV D FV IV E FV IV E FV IV E FV

LK LN LN LN LK LK LK LK LK

NTP TPS TPS TPS NLG NLG NTG NTG NTA

KMT2A_HUMAN (MLL) H3BFF4_LATCH A8VKP8_DANRE M4A2U4_XIPMA KMT2B_HUMAN (MLL2) J9NZ02_CANFA B0JZH9_XENTR F6XZK4_XENTR F6YNY3_XENTR

9aaTAD pattern of the MLL family: [SNQ][DE][LTI][LMV][DE][FL][STV][MKVAIL][MVKN]

Electronic Supplementary Material (ESI) for Molecular BioSystems. This journal is © The Royal Society of Chemistry 2014

Electronic Supplementary Material (ESI) for Molecular BioSystems. This journal is © The Royal Society of Chemistry 2014

Electronic Supplementary Material (ESI) for Molecular BioSystems. This journal is © The Royal Society of Chemistry 2014

Figure S6

Gcn4 family Leu 9aaTAD PIP ELD AA VV E SF FS SSTDSTP PLN ELD SN VV D AF FS SSTDSTP NNN ELD SA VV D AF FS STSDSTP ASP EVS AA LV D AF FS SSADSTP AYQ EID SA IV D AF FS SSTENTP NYN ELD SA VV D AF FS SSADSTP DNN DID AA VV D AF FS SSTDSTP TAA DAE SL VA D HF LA SSADSTP STT ELN AS IV E TF FD AASSTDSTP SAT ELS AS IV D NF FD PSSSTDSTP RST DL DS AV E NF FG SSSDSTP KSST DL DS AV E NF FG SSSDSTP NTV ELS SH VV E SF FE SSSDSTP GYE DID SA TV D AF FS SSTDSTP NST ELD AD LV E TF FS SSVDSTP SVP ELD QA VV D EF FV NDDAP EAP FVH SS VL D SV FS SNLDEADP SSL AFH SE VL D SV FS NDEAVDHT PL QLH SS IV E SI FS PSLVESTP PL QLH SS VV E SI FD PIMENQSP PL HVQ SS VL D SV FS TTLEG PL EIH TS VL D SV LS TDFTDAD PI IVH SE VL D SV FS TNLDDN PF NIH SS VL D SV FS STMDEN PF EIH SS VI D SI FE DPDAQAAP PF NIH SA VL D SV FS NVLDEHD PM PIH SS VL D SV FS CTMDELEN PF QIH SS VL E SV FS TNLDGVN PF EIH SD VL D SI FS TNLDGTN PL EIH AS TI D SV FD SALMPDEAP PF QIH SS VL E SV FS TNLDGVN PG CLG AE IA D SF FL EDEQA PF QIH SS VL D SV FS SNMDGSN PF QIH SS VL E SG FS TNLDGVN PF QIH SS VL E SV FS TNLHGVN PF QIH SS VL E SV FS TNLDGKD PF QIH SS VL D SV FS TTNLDGK

A6ZQT9_YEAS7 G0VJK4_NAUCC G0W5D3_NAUDC J7RCV7_KAZNA G8BZU6_TETPH A7TQ76_VANPO Q6FLL6_CANGA C5DLF6_LACTC Q6CQU7_KLULA W0TBM9_KLUMA R9XE39_ASHAC M9N3K0_ASHG1 G8JU33_ERECY I2GUU6_TETBL H2B208_KAZAF W0VM48_ZYGBA G8YPT9_PICSO G3B807_CANTC A0A061AP43_CYBFA K0KP45_WICCF A5DEW1_PICGU C4QY58_PICPG C4YB11_CLAL4 Q6BW79_DEBHA W1QLF0_OGAPD A3LU14_PICST G3AMH4_SPAPN C4YKR0_CANAW C5M582_CANTT W6MGB6_9ASCO Q9UV12_CANAX C5DVT1_ZYGRC M3K219_CANMX Q59YG8_CANAL B9WCQ8_CANDC H8X2T6_CANO9 A5DYV6_LODEL

9aaTAD pattern of the Gcn4 family: [ASTDQ][ALSDENH][LIVAT][LIVA][DE][STEAHN][FIVG][FL][SALVDEG]

Oaf1 family Leu GG L GS L GG L DN L GN L GR L GG L AT L GG L GG L SS L QS L DD L DI L HG V LA LA FL LN

-

G G G G

DL DF DL DF DI DL DL GV DI NL GV GI DL DF SV VS LS DI DF

9aaTAD FD Y DF FD Y DL FD Y EF LD Y DL FN Y DF FN Y DF FD Y DF LD F EF FD F LF FD Y DF SE F DL SD F DG LD Y EF FD T FS LN D YF AD F DI AN F DF FD R LM FN S AI

LF LF LF FF FF LF LF LL GN LF LF FL LF MN KQ VF VF GT IE

OAF1_YEAST PIP2_YEAST H2AZK4_KAZAF H2ASY9_KAZAF J7S6A9_KAZNA I2H429_TETBL G8C0X6_TETPH Q6FP27_CANGA W0VT70_ZYGBA C5DSJ9_ZYGRC G0VJL5_NAUCC G0W5E4_NAUDC R9XEX1_ASHAC W0TI53_KLUMA Q6CY39_KLULA W0TA41_KLUMA Q6CY40_KLULA K0KVI9_WICCF K0KRW3_WICCF

9aaTAD pattern of the Oaf1 family: [DNGSLV][LIVFS][LFSA][DN][YFTDRS][DELFA][FILMGS][LIFVMGK][FLNQ TE]

Pdr1 family Leu 9aaTAD GD L ED LY S IL WS PDR1_YEAST GD L TD LY H TL WN PDR3_YEAST TT M DD VY N YL FD GAL4_YEAST* TD M ND LY N VL WS G0V5I5_NAUCC TD L SD LY N VL WS G0WA57_NAUDC AD I DD LY N VL WG G0WA58_NAUDC GD L ND LY S TL WS W0VXU5_ZYGBA GD L ND LY T TL WS C5E070_ZYGRC GD L NE LY N SL WG Q1PSH2_CANGB SD M TD LC N IL WS H2AX37_KAZAF GD L ND LC N TL WS G8ZWQ0_TORDC TD L ND LY S VL WN G0V626_NAUCC GD L TE LY H TL WN J4TTH2_SACK1 GD L ND LY S TL WS W0VXU5_ZYGBA TD M ND LF K TL WN H2AP04_KAZAF AD I DD LY N VL WG G0WA58_NAUDC TD M ND LY N VL WS G0V5I5_NAUCC TD L ND LY S VL WN G0V626_NAUCC GD L ND LY T TL WS C5E070_ZYGRC SD M TD LC N IL WS H2AX37_KAZAF TD L ND LY D QL WS G0V625_NAUCC GD L ND LC N TL WS G8ZWQ0_TORDC TD L SD LY N VL WS G0WA57_NAUDC GD L NE LY N SL WG Q1PSH2_CANGB 9aaTAD pattern of the Pdr1 family: [DENST][DE]L[YCF][STHKND][ISTVQ]LW[SNG] * Pdr1 clostes homolog Gal4 was aligned Gal4 family Leu 9aaTAD TT M DD VY N YL FD GAL4_YEAST TT M DD VY D YL FD J8PVU2_SACAR TT M DD VY S YL FD G0VHW3_NAUCC TT M DD VY N FL FD C5E4R9_ZYGRC TT M DD IF N YV FN C5E274_LACTC TT M DD VY N YI FD LAC9_KLULA TT M DD VY N YI FD M4YCE1_KLUMA TT L DD VY T YL FN G0WFT2_NAUDC TT I DD VY N YL FD G0VEK9_NAUCC EL L ND IY S ML FD W1QGS8_OGAPD EW M TD IY S LF FN A0A060T6Y5_BLAAD EF V ND FY S LI FN K0KTZ9_WICCF TP Q ED FY S MM FS A0A061AR74_CYBFA 9aaTAD pattern of the Gal4 family: [DENT]D[IFV][YF][STND][YMLF][MLIV]F[DNS]

p53 family Leu MEEPQSDPSVEPPLSQ ET MEEPQSDPSIEPPLSQ ET MEEAQSDLSIEPPLSQ ET MEEPHSDLSIEPPLSQ ET MEESQSDLSLEPPLSQ ET MEEPQSDLSIELPLSQ ET MEESQSELGVEPPLSQ ET MEEPQSDLSIELPLSQ ET MEESQAELGVEPPLSQ ET MQEPPLELTIEPPLSQ ET MEESQSELNIDPPLSQ ET MEESQAELNVEPPLSQ ET MEESQSDISLELPLSQ ET MEDSQSDMSIELPLSQ ET MEPSSETGMD PPLSQ ET MEEADLT LPLSQ DT MEEADLT LPLSQ DT MADLAENVS LPLSQ ES MEEENIS LPLSQ DT MAEEMEP LLEPT EV

9aaTAD-I FS D LW KL FS D LW KL FS D LW NL FS D LW KL FS D LW KL FS D LW KL FS D LW KL FS D LW KL FS D LW NL FS E LW NL FS E LW NL FS D LW NL FS G LW KL FS C LW KL FE D LW SL FH D LW NN FH D LW NN FE D LW KM FQ D LW DN FM D LW SM

LPENNVLSPLPSQA LPENNVLSPLPSQA LPENNVLSPVLSPP LPENNVLSDSLSPP LPENNLLTTSLNPP LPPNNVLSTLPSSDS LPENNLLSSELSLAA LPPNNVLSTLPSSDS LPENNLLSSELSAP LPENNVLSSELSSA LPENNVLSSELCPA LPENNLLSSELSAP LPPEDILPSPHC LPPDDILPTTATGSPNS LPDPLQTVTCR VFLSTENESLAPP VFLSTENESLPPP NLNLVAVQPPETES VSAPPISTIQTAAL LPYSMQQLPLPEDH

MD VD MD MD VD IE VN IE VD MN VD VD MD ME

D D D H D E D E D E E D D D

Leu LMLSP LMLSP LLLSS LLLSP LLSA LFLS LLL LFLS LLPYS LPLS LLLP LLPY LLLP LFLP

DD DD ED EE ED EN SP EN ED ED ES TD QD QD

9aaTAD-II IE Q WF TE LA Q WL TE VE N WF DK VA S WL GE VA N WL NE VA G WL ED VT N WL DE VT G WL ED VV T WL DE VA N WL DE VV N WL DE VA T WL DE VE E FF EG VA E LL EG

DPGP DPGP GP NP DP PG NP SG CP AP DS CP PS PE

9aaTAD pattern of the p53 family: p53TAD-I [DE][TSV]F[SEHQM][DE]LW[KNSD][LMN] p53TAD-II

[DESTQ][DESNP][ILV][ETA][QNGSTE][WFL][FL][DETG][DEGK]

p53TADs

[DESTQ][DETSNVP][FILV][ESTAMQH][QNGSTDE][WFL][WFL][DESTGKN][DEGLMNK]

P53_HUMAN P53_MACMU P53_MARMO P53_CAVPO P53_RABIT P53_MESAU P53_PIG P53_CRIGR P53_SHEEP P53_FELCA P53_CANFA P53_BOVIN P53_MOUSE P53_RAT P53_XENLA P53_XIPHE P53_XIPMA P53_ONCMY P53_TETMU P53_CHICK