Electrophoresis File Run Summary

2100 expert_Eukaryote Total RNA Nano_DE72902887_2017-06-01_13-31-42.xad Assay Class: Data Path:

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Eukaryote Total RNA Nano E:\...Eukaryote Total RNA Nano_DE72902887_2017-06-01_13-31-42.xad

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Electrophoresis File Run Summary

Instrument Information: Instrument Name: Serial#:

Assay Information: Assay Origin Path: Assay Class: Version:

Assay Comments: Chip Information:

DE72902887

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8

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Firmware: C.01.069

DE72902887

Type:

G2939A

C:\Program Files\Agilent\2100 bioanalyzer\2100 expert\assays\RNA\Eukaryote Total RNA Nano Series II.xsy Eukaryote Total RNA Nano 2.6

Total RNA Analysis ng sensitivity (Eukaryote)

© Copyright 2003 - 2009 Agilent Technologies, Inc.

Chip Lot #:

Reagent Kit Lot #: Chip Comments:

RIN N/A

RIN N/A

RIN N/A

RIN: 6.50

Sample 1

Sample 4

Sample 7

Sample 10

2100 Expert (B.02.07.SI532)

RIN N/A

RIN N/A

RIN N/A

RIN N/A

Sample 2

Sample 5

Sample 8

Sample 11


RIN N/A

RIN N/A

RIN N/A

RIN N/A

Sample 3

Sample 6

Sample 9

Sample 12

Printed:

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Electrophoresis File Run Summary (Chip Summary) Sample Name Sample Sample Sample Sample Sample Sample Sample Sample Sample Sample Sample Sample

1 2 3 4 5 6 7 8 9 10 11 12

Sample Comment

Chip Lot #

Statu Result Label s RIN N/A RIN N/A RIN N/A RIN N/A RIN N/A RIN N/A RIN N/A RIN N/A RIN N/A RIN: 6.50 RIN N/A RIN N/A

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Result Color

Reagent Kit Lot #

Chip Comments :

2100 Expert (B.02.07.SI532)




Electrophoresis Assay Details

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General Analysis Settings

Number of Available Sample and Ladder Wells (Max.) : 13 Minimum Visible Range [s] : 17 Maximum Visible Range [s] : 70 Start Analysis Time Range [s] : 19 End Analysis Time Range [s] : 69 Ladder Concentration [ng/µl] : 150 Lower Marker Concentration [ng/µl] : 0 Upper Marker Concentration [ng/µl] : 0 Used Lower Marker for Quantitation Standard Curve Fit is Logarithmic Show Data Aligned to Lower Marker

Integrator Settings

Integration Start Time [s] : 19 Integration End Time [s] : 69 Slope Threshold : 0.6 Height Threshold [FU] : 0.5 Area Threshold : 0.2 Width Threshold [s] : 0.5 Baseline Plateau [s] : 6

Filter Settings

Filter Width [s] : 0.5 Polynomial Order : 4

Ladder

Ladder Peak 1 2 3 4 5 6

Size

25 200 500 1000 2000 4000

2100 Expert (B.02.07.SI532)



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Electropherogram Summary

Ladder

Overall Results for Ladder RNA Area:

RNA Concentration:

Result Flagging Label:

Overall Results for sample 1 : 211.3

RNA Area:

189.4

rRNA Ratio [28s / 18s]:

0.0

RNA Concentration:

Fragment table for sample 1 :

RIN N/A

Sample 1

Name

Start Size [nt] End Size [nt] Area

% of total Area

18S

3,400

28.4

4,020

53.7

Sample 2

174.0


0.0

RNA Concentration:

Sample 3

Sample 2

Overall Results for sample 3 :

124 ng/µl

RNA Integrity Number (RIN): Result Flagging Label:


% of total Area

18S

3,411

30.2

2100 Expert (B.02.07.SI532)

0.0

Sample 3

209 ng/µl N/A (B.02.07)


Start Size [nt] End Size [nt] Area 52.5

rRNA Ratio [28s / 18s]: Result Flagging Color:

Name

4,005

294.0

RNA Integrity Number (RIN):

RIN N/A

Sample 2

RNA Area:

RNA Concentration:

N/A (B.02.07)

Result Flagging Color:

Sample 1

N/A (B.02.07)



RNA Area:

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134 ng/µl


All Other Samples


8

Sample 1

150 ng/µl


4 of


RIN N/A

Sample 3

Name


% of total Area

18S

3,399

4,028

84.4

28.7

28S

5,816

6,405

0.8

0.3


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Electropherogram Summary Continued ... Sample 4

Overall Results for sample 4 : RNA Area:

275.1


0.0

RNA Concentration:

Sample 4




% of total Area

18S

3,373

28.1

0.0

RNA Area:

386.6


0.0

RNA Concentration:


RIN N/A

Sample 5

Name


% of total Area

18S

3,358

3,957

112.5

28.6

28S

5,561

6,393

2.0

0.5

Sample 7

Sample 6


274 ng/µl




0.0

N/A (B.02.07)



% of total Area

18S

3,334

3,916

111.7

28.9

28S

5,702

6,342

1.2

0.3

Sample 7

3 ng/µl


Name

2100 Expert (B.02.07.SI532)

3.7


RIN N/A

Sample 6

RNA Area:

RNA Concentration:

N/A (B.02.07)


Sample 5

N/A (B.02.07)

Sample 6


6/1/2017 1:31:42 PM 6/1/2017 2:09:12 PM

279 ng/µl


Start Size [nt] End Size [nt] Area 77.4


Name

4,014

392.9


RIN N/A

Sample 4

RNA Area:

RNA Concentration:

N/A (B.02.07)


8

Sample 5

195 ng/µl


5 of


RIN N/A

Sample 7

Name


% of total Area

18S

3,384

73.0

3,951


2.7

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Electropherogram Summary Continued ... Sample 8


10.3


0.0

RNA Concentration:

Sample 8




12.0


Sample 8

0.0



% of total Area

18S

3,358

19.8


RIN N/A

Sample 9

Name


% of total Area

18S

3,309

22.5

3,766

2.7

Sample 10


25.0


0.0

RNA Concentration:

Sample 11

Sample 10


18 ng/µl



Sample 10

0.0

% of total Area

18S

3,300

13.1

Sample 11

13 ng/µl N/A (B.02.07)



2100 Expert (B.02.07.SI532)


RIN: 6.50

3.3

18.8


Name

3,740

RNA Area:

RNA Concentration:

6.5 (B.02.07)


Sample 9

N/A (B.02.07)


Name

2.0

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9 ng/µl


RIN N/A

3,909

RNA Area:

RNA Concentration:

N/A (B.02.07)


8

Sample 9

7 ng/µl


6 of


RIN N/A

Sample 11

Name


% of total Area

18S

3,255

30.7

3,737


5.8

Printed:

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Electropherogram Summary Continued ...

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Sample 12


23.5


0.0

RNA Concentration:

Sample 12

17 ng/µl


N/A (B.02.07)




RIN N/A

Sample 12

Name


% of total Area

18S

3,245

26.1

3,853

2100 Expert (B.02.07.SI532)

6.1



Gel Image


2100 Expert (B.02.07.SI532)


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Electrophoresis File Run Summary

Electrophoresis File Run Summary

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