Elevation of asymmetrical dimethylarginine may ... - Semantic Scholar

5 downloads 0 Views 117KB Size Report
... Stefanie M. BODE-BO$GER*,. Howard R. KNAPP† and William G. HAYNES† ..... and Whitley, G. S. J. (1993) Synthesis of NG,NG- dimethylarginine by human ...
Clinical Science (2001) 100, 161–167 (Printed in Great Britain)

Elevation of asymmetrical dimethylarginine may mediate endothelial dysfunction during experimental hyperhomocyst(e)inaemia in humans Rainer H. BO$ GER*, Steven R. LENTZ†, Stefanie M. BODE-BO$ GER*, Howard R. KNAPP† and William G. HAYNES† *Institute of Clinical Pharmacology, Hannover Medical School, 30623 Hannover, Germany, and †Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, IA-52242-1081, U.S.A.

A

B

S

T

R

A

C

T

Hyperhomocyst(e)inaemia is associated with endothelial dysfunction in animals and humans. Mechanisms responsible for endothelial dysfunction in hyperhomocyst(e)inaemia are poorly understood, but may involve impaired bioavailability of endothelium-derived nitric oxide (NO). We hypothesized that acute elevation of homocyst(e)ine by oral methionine loading may stimulate the formation of asymmetrical dimethylarginine (ADMA), an endogenous inhibitor of NO synthase, due to a transmethylation reaction during the formation of homocyst(e)ine from methionine. We studied nine healthy human subjects (five males, four females) aged 29p2 years. Flow-mediated vasodilation (FMD) in the brachial artery (endothelium-dependent) and vasodilation induced by nitroglycerine (endothelium-independent) were measured with highresolution ultrasound before and 8 h after oral methionine (100 mg/kg in cranberry juice) or placebo (cranberry juice), on separate days and in random order. Plasma homocyst(e)ine and ADMA concentrations were measured by specific HPLC methods. After a methionine bolus, elevation of homocyst(e)ine (28n4p3n5 µmol/l) was associated with an increased plasma concentration of ADMA (2n03p0n18 µmol/l) and reduced FMD (1n54p0n92 %). Placebo had no effect on these parameters. There was a significant inverse linear relationship between ADMA concentration and FMD (r lk0n49 ; P 0n05), which was stronger than the relationship between the homocyst(e)ine concentration and FMD (r lk0n36 ; not significant). We conclude that acute elevation of the homocyst(e)ine concentration impairs vascular endothelial function by a mechanism in which an elevated concentration of ADMA may be involved. This finding may have importance for understanding the mechanism(s) leading to homocyst(e)ine-associated vascular disease, and its potential treatment.

INTRODUCTION Hyperhomocyst(e)inaemia, a major and independent risk factor for cardiovascular disease, is associated with

endothelial dysfunction [1,2], the mechanisms of which are not fully understood. The term ‘ hyperhomocyst(e)inaemia ’ is used in this paper to indicate that plasma homocyst(e)ine assays measure the total concentration of

Key words : atherosclerosis, coronary disease, endothelial function, nitric oxide, ultrasound. Abbreviations : ADMA, asymmetrical dimethylarginine ; DDAH, dimethylarginine dimethylaminohydrolase ; FMD, flowmediated vasodilation ; SDMA, symmetrical dimethylarginine. Correspondence : Dr Rainer H. Bo$ ger, Clinical Pharmacology Unit, Department of Pharmacology, Institute of Experimental and Clinical Pharmacology and Toxicology, University Hospital Hamburg-Eppendorf, Martinistr. 52, D-20246 Hamburg, Germany (e-mail boeger!uke.uni-hamburg.de).

#

2001 The Biochemical Society and the Medical Research Society

161

162

R. Bo$ ger and others

thiol, disulphide and mixed-disulphide adducts of homocysteine. In high concentrations, homocyst(e)ine may be directly toxic to cultured endothelial cells [3], but differences in homocyst(e)ine concentrations observed in clinical trials between cases and controls were much smaller [4,5]. Evidence suggests that decreased biological activity of endothelium-derived nitric oxide (NO) may be involved in homocyst(e)ine-associated endothelial dysfunction (for a review, see [6]). Stamler et al. [7] postulated that the redox activity of homocyst(e)ine may contribute to enhanced oxidative inactivation of NO. Based on the results of a study in non-human primates, we have hypothesized that the generation of asymmetrical dimethylarginine (ADMA), an endogenous inhibitor of NO synthase [8], may be a novel mechanism contributing to the endothelial dysfunction associated with hyperhomocyst(e)inaemia [9]. An elevated plasma level of ADMA is a risk factor for endothelial dysfunction in hypercholesterolaemia [10] and atherosclerosis [11]. ADMA is synthesized by methylation of the side-chain guanidino nitrogen atoms of L-arginine residues within proteins, due to the action of methyltransferases that utilize S-adenosylmethionine as a methyl group donor [12]. S-Adenosylmethionine is an intermediate in the demethylation of methionine to homocyst(e)ine, which is the major pathway supplying methyl groups for various methylation reactions (for a review, see [13]). Acute hyperhomocyst(e)inaemia can be induced by oral methionine loading in healthy humans, and produces endothelial dysfunction [14–16]. Our hypothesis was that increased availability of S-adenosylmethionine during experimental hyperhomocyst(e)inaemia induced by oral methionine loading may stimulate the formation of ADMA, which will then induce endothelial dysfunction by inhibiting endothelial NO synthase. The present study was undertaken to test whether elevation of ADMA plasma levels is associated with endothelial dysfunction during experimental hyperhomocyst(e)inaemia (induced by acute methionine loading) in healthy humans.

METHODS Subjects and study protocol This was a two-phase, randomized, double-blind, placebo-controlled, cross-over study comparing the effects of oral L-methionine and placebo on endothelial function, with a washout period of at least 1 week between the two study days. The investigation conformed with the principles outlined in the Declaration of Helsinki. The studies were conducted after obtaining written informed consent from each subject, and with the approval of the University of Iowa Institutional Review Board. Nine healthy subjects, without risk factors for, or #

2001 The Biochemical Society and the Medical Research Society

clinical evidence of, atherosclerosis, were recruited by advertisement. No subject received vasoactive drugs in the week before the study, and all abstained from alcohol for 24 h, and from caffeine for at least 12 h, before any measurements. Subjects were admitted to the University of Iowa General Clinical Research Center in the evening, and studied in a fasted state the next morning. Studies were performed in a quiet room maintained at a constant temperature of between 22 and 25 mC. An antecubital vein was cannulated with a 22G catheter for blood sampling. Subjects received oral L-methionine (100 mg\ kg ; Ajinomoto, Teaneck, NJ, U.S.A.) dissolved in cranberry juice, or cranberry juice alone, at approx. 07.00 hours. A standard breakfast containing 58 mg of Lmethionine and 5 g of fat was served 2 h after methionine or placebo administration. Conduit vessel vasomotor function was assessed 8 h after L-methionine\placebo administration. Venous blood for assay of plasma homocyst(e)ine and methionine was obtained before and 8 h after L-methionine\placebo administration.

Endothelium-dependent vasodilation To study endothelial function in conduit vessels, flowmediated vasodilation (FMD) of the brachial artery was induced using reactive hyperaemia as a stimulus [2,17,18]. A 5 cm length of the brachial artery was imaged in longitudinal section above the antecubital fossa using an ultrasound-Doppler machine with a 7n5 MHz linear array transducer (Toshiba SSA-270, Andover, MA, U.S.A.). In order to improve reproducibility, we used the same ultrasonographer for image acquisition, a standard arm support and identical probe distance from the antecubital fossa (2n5 cm proximal). Arterial flow velocity measurements were made by a pulsed Doppler signal at an " 70m angle to the vessel. Measurements were obtained for 20 s at baseline. An occluding forearm cuff placed 5 cm below the antecubital fossa was inflated to 50 mmHg above systolic pressure for 5 min and then released to induce reactive hyperaemia. Recordings were made 5, 60 and 120 s after the onset of reactive hyperaemia. Brachial artery diameter and flow velocity were also measured before and for 6 min after sublingual administration of nitroglycerine spray (400 µg). Blood pressure was recorded using an automated sphygmomanometer (LifeStat 200 ; Physio Control, Redmond, WA, U.S.A.).

Biochemical analyses Plasma homocyst(e)ine assays were performed in the University of Iowa General Clinical Research Center Core Lab, using a modification of the assay described by Noguchi and Higuchi [19]. Samples were collected in EDTA tubes and kept at 4 mC until the plasma was separated and frozen at k70 mC. Plasma was spiked with

Homocyst(e)ine, asymmetrical dimethylarginine and endothelial dysfunction

known amounts of internal standard (mercaptopropionyl glycine), reduced with tri-n-butyl phosphine and then deproteinized with sulphosalicylic acid. The thiolspecific fluorigenic labelling reagent ammonium 7fluorobenzo-2-oxa-1,3-diazole-4-sulphonate was added to the supernatant, and the sample was neutralized with HCl, washed with a 5 % tributyl phosphate solution, filtered and injected on to a reverse-phase HPLC column. Quantification was by measurement of the emission signal of the analyte at 515 nm (excitation 385 nm). The coefficient of variation was 2 %. The results of this assay are expressed as plasma homocyst(e)ine, and represent the sum of free and bound forms of homocysteine, homocystine and homocysteine\cysteine mixed disulphide. Plasma methionine was measured with an automated amino acid analyser (Beckman 7300 ; Beckman Instruments, Palo Alto, CA, U.S.A.) using a lithium physiological ion-exchange column system. Quantification was achieved by post-column derivatization with ninhydrin, monitoring UV absorbance with norleucine as an internal standard. Concentrations of L-arginine and dimethylarginines were determined by HPLC using pre-column derivatization with o-phthalaldehyde by a modification of a previously described method [20]. Samples were spiked with 10 µM homoarginine as internal standard, and extracted on CBA solid-phase extraction cartridges (Varian, Harbor City, CA, U.S.A.). The eluates were dried under nitrogen and the residues were dissolved in double-distilled water for HPLC analysis. HPLC was carried out on a Gynkotek liquid chromatography system (Gynkotek, Munich, Germany) consisting of two HPLC pumps with a gradient controller (model M 480 HDG), a spectral fluorescence detector (RF 1002) and an automatic injector (model GINA 160). Samples and standards were incubated for exactly 30 s with the ophthaladehyde reagent (5n4 mg\ml in borate buffer, pH 8n5, containing 0n4 % 2-mercaptoethanol) before automatic injection into the HPLC system. Chromatographic separation was performed on a C H column ' & (Macherey and Nagel, Du$ ren, Germany) with the fluorescence monitor set at λex l 340 nm and λem l 455 nm. Samples were eluted from the column isocratically with 0n96 % citric acid\methanol (2 : 1, v\v, pH 6n8) at a flow rate of 1 ml\min. The coefficients of variation of this method were 5n2 % (within-assay) and 5n5 % (between-assay) ; the detection limit of the assay was 0n1 µmol\l.

Calculations and statistical analyses All analyses were performed by observers blinded to treatment assignment. Brachial artery diameter and blood velocity measurements were analysed using a vascular ultrasound Doppler analysis package provided with the Toshiba machine. For each of three consecutive cardiac

cycles, brachial artery diameter (mm) was calculated as the mean of three evenly spaced measurements of the distance from the trailing edge to the leading edge of ultrasonic arterial borders at end-diastole, using electronic calipers on the ultrasound machine. Mean Doppler flow velocity (cm\s) over the entire cardiac cycle was calculated and averaged over three consecutive cardiac cycles. Percentage changes in diameter and velocity were calculated 10 s, 1 min and 2 min after the induction of reactive hyperaemia, and 2, 4 and 6 min after nitroglycerine. In 40 studies performed in healthy subjects with no interventions, dilation in response to reactive hyperaemia was maximal at 2 min after cuff deflation (1 min, 5n6p0n7 % ; 2 min, 6n2p0n6 %), so the 2 min time point is shown in Figures and Tables. Maximum dilation in response to nitroglycerine occurred 6 min after administration (2 min, 6n2p0n7 % ; 4 min, 14n1p1n4 % ; 6 min, 14n5p1n5 %), so the 6 min time point is shown in Figures and Tables. Our methodology for the assessment of conduit vessel FMD has good within-subject reproducibility, with a correlation coefficient of 0n75 between ten paired studies, and an average coefficient of variability of 13n6 % in three subjects studied on four or more occasions. All data are given as meanspS.E.M. Statistical significance was tested using ANOVA followed by Fisher’s protected least significant difference test. Statistical significance was accepted at P 0n05.

RESULTS Subjects were normohomocyst(e)inaemic at baseline. Methionine loading significantly increased plasma methionine and homocyst(e)ine concentrations as compared with placebo (Table 1). The ADMA concentration was also increased significantly (by 69n8p26n2 %) after methionine, but not after placebo (17n2p14n2 % ; P 0n05 between treatments). There was no association between ADMA and methionine levels. Symmetrical dimethylarginine (SDMA) and L-arginine levels were not changed significantly, resulting in a significantly decreased Larginine\ADMA ratio after methionine loading (Table 1). There was a significant association between the plasma concentrations of homocyst(e)ine and ADMA in individual subjects (r l 0n48, P 0n05 ; Figure 1). FMD was significantly decreased after methionine (1n54p0n92 %) as compared with after placebo (7n08p1n56 % ; P 0n05). Flow velocity in the brachial artery during hyperaemia was unchanged (Table 2). Endothelium-independent vasodilation induced by nitroglycerine was also unchanged (Table 2). FMD was inversely correlated with ADMA levels (r lk0n49, P 0n05 ; Figure 2) and positively correlated with the Larginine\ADMA ratio (r l 0n60, P 0n01 ; Figure 3). By #

2001 The Biochemical Society and the Medical Research Society

163

164

R. Bo$ ger and others

Table 1

Plasma methionine, homocyst(e)ine, L-arginine and dimethylarginine concentrations

Data are meanspS.E.M. Significance of differences : *P

0n05 compared with baseline.

Concentration ( µmol/l) Methionine

Methionine Homocyst(e)ine ADMA SDMA L-Arginine L-Arginine/ADMA ratio

Placebo

Baseline

8h

Baseline

8h

20n4p1n6 6n9p0n6 1n39p0n20 1n70p0n25 53n2p7n3 41n2p4n4

353n3p56n6* 28n4p3n5* 2n03p0n18* 1n98p0n19 66n7p6n9 33n0p1n9*

23n0p2n2 6n9p0n4 1n70p0n21 1n89p0n28 57n1p5n7 35n9p2n6

19n8p1n4 7n3p0n4 1n81p0n18 1n91p0n24 70n1p6n0 40n4p3n0

Table 2 Ultrasonographic determination of FMD and nitroglycerine-induced vasodilation after methionine and placebo

Data are meanspS.E.M. for nine subjects, and are expressed as the percentage change induced by hyperaemia or by nitroglycerine. Significance of difference compared with placebo : *P 0n05. Change (%) Methionine FMD Flow velocity Diameter Nitroglycerine Flow velocity Diameter

Figure 1 Effects of oral methionine on plasma homocyst(e)ine and ADMA concentrations

Upper panel : changes in plasma homocyst(e)ine and ADMA concentrations for each of nine volunteers before and 8 h after oral methionine loading. Lower panel : relationship between the change in the plasma ADMA concentration and the change in the plasma homocyst(e)ine concentration 8 h after methionine ($) or placebo (#). #

2001 The Biochemical Society and the Medical Research Society

385p43 1n54p0n92* k2n4p4n9 16n4p2n0

Placebo 411p48 7n08p1n56 4n6p3n4 14n1p1n1

Figure 2 Correlation analysis for plasma ADMA concentration and FMD vasodilation in nine healthy human subjects 8 h after methionine ($) or placebo (#)

contrast, the association between homocyst(e)ine concentration and FMD was much weaker and not statistically significant (r l 0n36).

Homocyst(e)ine, asymmetrical dimethylarginine and endothelial dysfunction

Figure 3 Correlation analysis for plasma L-arginine/ADMA ratio and FMD in nine healthy human subjects 8 h after methionine ($) or placebo (#)

DISCUSSION Our main finding is that an acute increase in plasma homocyst(e)ine levels due to oral methionine loading was associated with a significant increase in plasma levels of ADMA, an endogenous inhibitor of NO synthase. Impairment of brachial artery FMD after methionine loading was correlated with ADMA levels, suggesting that ADMA may mediate impaired endotheliumdependent vasodilation during experimental hyperhomocyst(e)inaemia in humans. Brachial artery FMD is largely dependent upon endothelial NO elaboration [21]. Impairment of NOmediated vasodilation is a sensitive indicator of the atherosclerotic process, since it occurs early during the development of atherosclerosis [22], and improves early during the regression of atherosclerotic lesions [23,24]. In humans, the degree of endothelial dysfunction is associated with the number of cardiovascular risk factors present in an individual [25]. Therefore endotheliumdependent vasodilation can be regarded as a surrogate marker for atherosclerosis risk. Controlled studies in humans and in non-human primates have clearly shown an association between hyperhomocyst(e)inaemia and impaired endotheliumdependent, NO-mediated vasodilation. Lentz et al. [1] found that monkeys fed on a diet enriched in methionine and deficient in folate and choline exhibited impaired endothelium-dependent vasodilation in vivo and ex vivo. Acute hyperhomocyst(e)inaemia induced by an oral methionine load impairs endothelium-dependent vasodilation in human subjects [14–16]. In the study by Kanani et al. [16], flow-mediated vasodilation was inversely related to plasma homocyst(e)ine, with r l 0n49

(P 0n01), in 40 healthy subjects. By contrast, Chambers et al. [26] found no significant correlation between postload plasma homocyst(e)ine and FMD (r lk0n42, P  0n05) in 17 healthy subjects. Bellamy and co-workers [14] studied 24 healthy humans before and after an acute oral methionine load, and also found that endotheliumdependent vasodilation was significantly impaired simultaneously with an increased plasma homocyst(e)ine concentration. However, that study and our present study found no significant correlation between the change in FMD and the plasma homocyst(e)ine concentration. The discrepancy between studies may be related to the number of subjects tested and to the timing of measurements. This discrepancy also suggests that the link between homocyst(e)ine and endothelium-derived NO may be indirect. This conclusion is further corroborated by a study showing that dietary supplementation with B vitamins did not reverse a pre-existing dysfunction in endothelium-dependent vasodilation in monkeys with diet-induced hyperhomocyst(e)inaemia and hypercholesterolaemia [27]. We recently found an association between homocyst(e)ine levels and plasma concentrations of ADMA in monkeys with diet-induced hyperhomocyst(e)inaemia [9]. In these animals, multivariate analyses revealed that the degree of endothelial dysfunction was associated more closely with ADMA levels than with homocyst(e)ine levels. The synthesis of homocyst(e)ine from methionine involves demethylation of the intermediate, S-adenosylmethionine [13]. S-Adenosylmethionine is a ubiquitous methyl group donor, and therefore we speculated that dimethylation of L-arginine to form ADMA may utilize S-adenosylmethionine as a methyl group donor. Methionine loading increases substrate availability for methyltransferases, thereby facilitating the synthesis of ADMA. We recently demonstrated that "%C-labelled methyl groups from [methyl "%C]Sadenosylmethionine can be incorporated into ADMA by cultured human endothelial cells [28]. In that study, incubation of endothelial cells with methionine induced a concentration-dependent increase in ADMA release, which was inhibited by the N-methyltransferase inhibitor S-adenosylhomocysteine [28]. However, because methionine concentrations were higher and the incubation period was longer than the respective plasma concentration and time after oral methionine bolus in the present clinical study, we performed additional incubation experiments with human EAhy.926 endothelial cells. After incubation with L-methionine at a concentration similar to that reached in the plasma of human volunteers after oral methionine loading for 8 h (i.e. 300 µM), we found that ADMA release was slightly, but significantly, increased compared with the control (control, 7n1p0n4 pmol of ADMA\µg of protein ; methionine, 10n0p0n6 pmol of ADMA\µg of protein ; each n l 6 ; P 0n01). Inhibition of S-adenosylmethionine#

2001 The Biochemical Society and the Medical Research Society

165

166

R. Bo$ ger and others

dependent methyltransferase also reduced baseline ADMA formation and the low-density-lipoproteincholesterol-induced increase in ADMA release in cultured human endothelial cells [28]. N-Methyltransferases present in human endothelium specifically asymmetrically methylate L-arginine residues, resulting in the release of ADMA and NG-monomethyl-L-arginine, but not SDMA [28]. Thus there is strong experimental evidence that this mechanism (i.e. elevation of ADMA formation due to increased N-methyltransferase activity) also plays a role in endothelial dysfunction after acute methionine loading in human subjects. Another possible explanation for the selective increase in ADMA levels after methionine loading may be decreased activity of dimethylarginine dimethylaminohydrolase (DDAH), an enzyme which metabolizes ADMA, but not SDMA, to citrulline and dimethylamine [29]. However, neither methionine nor homocysteine modifies DDAH activity in cultured endothelial cells in vitro (R. H. Bo$ ger, unpublished work), making it improbable that this is the molecular mechanism underlying our present observations. The increase in the plasma concentration of ADMA evoked by oral methionine loading in the present study (1n4 to 2n0 µmol\l) may appear rather limited and insignificant at first sight. However, there is evidence that such small differences in plasma ADMA concentrations can induce significant differences in endothelial function [10]. Moreover, experimental data suggest that ADMA levels within endothelial cells (i.e. at the site of NO production by endothelial NO synthase) may be much higher than those measured extracellularly [30,31]. Additional evidence indicates that inhibition of DDAH activity, which increases endogenous ADMA levels within cells, inhibits endothelium-dependent relaxation in isolated arterial rings [32], suggesting that ADMA is involved in the regulation of NO synthase activity. Experimental data suggest that homocyst(e)ine directly alters the biological availability of endothelial NO by a mechanism involving oxidative inactivation of this molecule [33]. Upchurch et al. [34] found that homocysteine impairs the ability of cultured bovine aortic endothelial cells to detoxify hydrogen peroxide, thus rendering NO more susceptible to oxidative inactivation. Our group has shown that endothelial dysfunction caused by methionine loading can be reversed by administration of an antioxidant (ascorbic acid) [16]. However, other investigators failed to show increased oxidative stress during acute or medium-term supplementation with oral methionine [35]. Our present finding that ADMA is involved in homocyst(e)ine-induced endothelial dysfunction is not in conflict with the observations of elevated oxidative stress during experimental hyperhomocyst(e)inaemia as cited above. It is known that inhibition of endothelial NO synthase shifts the balance between NO and O −

#

#

2001 The Biochemical Society and the Medical Research Society

in favour of oxidative stress [36]. Bo$ ger et al. [31] reported that incubation of human endothelial cells with ADMA increases endothelial superoxide release and leads to the activation of redox-regulated genes. Lin et al. [37] extended this finding by showing that inhibition of the metabolism of endogenous AMDA by the enzyme DDAH increased the ADMA concentration in conditioned media and also enhanced endothelial superoxide release. Finally, Xiong et al. [38] showed that elevation of dimethylarginine levels in cholesterol-fed rabbits was associated with lipid peroxidation. Our present findings, therefore, suggest that L-arginine may function as a methyl group acceptor during the demethylation of methionine to homocyst(e)ine, resulting in increased formation of ADMA. ADMA acts as an endogenous inhibitor of endothelial NO synthase [8], and an elevated ADMA concentration constitutes a risk factor for endothelial dysfunction in humans [10]. Thus our findings suggest a novel mechanism for impaired endothelial function associated with hyperhomocyst(e)inaemia.

ACKNOWLEDGMENTS We gratefully acknowledge the excellent technical assistance of B. Schubert. This study was supported in part by a grant from the Deutsche Forschungsgemeinschaft (DFG Bo 1431\3-1) and by the National Institutes of Health (NHLBI HL-58972). W. G. H. is the recipient of a Faculty Development Award from the Pharmaceutical Research Manufacturers of America Foundation.

REFERENCES 1 Lentz, S. R., Sobey, C. G., Piegors, D. J. et al. (1996) Vascular dysfunction in monkeys with diet-induced hyperhomocyst(e)inemia. J. Clin. Invest. 98, 24–29 2 Tawakol, A., Omland, T., Gerhard, M., Wu, J. T. and Creager, M. A. (1997) Hyperhomocyst(e)inemia is associated with impaired endothelium-dependent vasodilation in humans. Circulation 95, 1119–1121 3 de Groot, P. G., Willems, C., Boers, G. H. J., Gonsalves, M. D., van Aken, W. G. and van Mourik, J. A. (1983) Endothelial cell dysfunction in homocystinuria. Eur. J. Clin. Invest. 13, 405–410 4 Clarke, R., Daly, L., Robinson, K. et al. (1991) Hyperhomocysteinemia : An independent risk factor for vascular disease. N. Engl. J. Med. 324, 1149–1155 5 Stampfer, M. J., Malinow, M. R., Willet, W. C. et al. (1992) A prospective study of plasma homocyst(e)ine and risk of myocardial infarction in US physicians. JAMA J. Am. Med. Assoc. 268, 877–881 6 Lentz, S. R. (1997) Homocysteine and vascular dysfunction. Life Sci. 61, 1205–1215 7 Stamler, J. S., Osborn, J. A., Jaraji, O. et al. (1993) Adverse effects of homocysteine are modulated by endotheliumderived relaxing factor and related oxides of nitrogen. J. Clin. Invest. 91, 308–318

Homocyst(e)ine, asymmetrical dimethylarginine and endothelial dysfunction

8 Vallance, P., Leone, A., Calver, A., Collier, J. and Moncada, S. (1992) Endogenous dimethylarginine as an inhibitor of nitric oxide synthesis. J. Cardiovasc. Pharmacol. 20 (Suppl. 12), S60–S62 9 Bo$ ger, R. H., Bode-Bo$ ger, S. M., Sydow, K., Heistad, D. D. and Lentz, S. R. (2000) Plasma concentration of asymmetric dimethylarginine, an endogenous inhibitor of nitric oxide synthase, is elevated in monkeys with hyperhomocyst(e)inemia. Arterioscler. Thromb Vasc. Biol. 20, 1557–1564 10 Bo$ ger, R. H., Bode-Bo$ ger, S. M., Szuba, A. et al. (1998) Asymmetric dimethylarginine : a novel risk factor for endothelial dysfunction. Its role in hypercholesterolemia. Circulation 98, 1842–1847 11 Bo$ ger, R. H., Bode-Bo$ ger, S. M., Thiele, W., Junker, W., Alexander, K. and Fro$ lich, J. C. (1997) Biochemical evidence for impaired nitric oxide synthesis in patients with peripheral arterial occlusive disease. Circulation 95, 2068–2074 12 Gary, J. D. and Clarke, S. (1998) RNA and protein interactions modulated by protein arginine methylation. Prog. Nucleic Acid Res. Mol. Biol. 61, 65–131 13 Malinow, M. R. (1994) Homocyst(e)ine and arterial occlusive diseases. J. Intern. Med. 236, 603–617 14 Bellamy, M. F., McDowell, I. F. W., Ramsey, M. W. et al. (1998) Hyperhomocysteinemia after an oral methionine load acutely impairs endothelial function in healthy adults. Circulation 98, 1848–1852 15 Chambers, J. C., McGregor, A., Jean-Marie, J. and Kooner, J. S. (1998) Acute hyperhomocysteinaemia and endothelial dysfunction (letter). Lancet 351, 36–37 16 Kanani, P. M., Sinkey, C. A., Browning, R. L., Allaman, M., Knapp, H. R. and Haynes, W. G. (1999) Role of oxidant stress in endothelial dysfunction produced by experimental hyperhomocyst(e)inemia in humans. Circulation 100, 1161–1168 17 Anderson, E. A. and Mark, A. L. (1998) Flow-mediated and reflex changes in large peripheral artery tone in humans. Circulation 79, 93–100 18 Corretti, M. C., Plotnick, G. D. and Vogel, R. A. (1995) Technical aspects of evaluating brachial artery vasodilatation using high-frequency ultrasound. Am. J. Physiol. 268, H1397–H1404 19 Noguchi, K. and Higuchi, S. (1992) Determination of glutathione isopropyl ester in rat, dog, and human blood by high-performance liquid chromatography with fluorescence detection. J. Pharm. Biomed. Anal. 10, 151–152 20 Bode-Bo$ ger, S. M., Bo$ ger, R. H., Kienke, S., Junker, W. and Fro$ lich, J. C. (1996) Elevated L-arginine\ dimethylarginine ratio contributes to enhanced systemic NO production by dietary L-arginine in hypercholesterolemic rabbits. Biochem. Biophys. Res. Commun. 219, 598–603 21 Joannides, R., Haefeli, W. E., Linder, L. et al. (1995) Nitric oxide is responsible for flow-dependent dilatation of human peripheral conduit arteries in vivo. Circulation 91, 1314–1319 22 Otsuji, S., Nakajima, O., Waku, S. et al. (1995) Attenuation of acetylcholine-induced vasoconstriction by L-arginine is related to the progression of atherosclerosis. Am. Heart J. 129, 1094–1100

23 Benzuly, K. H., Padgett, R. C., Kaul, S., Piegors, D. J., Armstrong, M. L. and Heistad, D. D. (1994) Functional improvement precedes structural regression of atherosclerosis. Circulation 89, 1810–1818 24 Sobey, C. G., Faraci, F. M., Piegors, D. J. and Heistad, D. D. (1996) Effect of short-term regression of atherosclerosis on reactivity of carotid and retinal arteries. Stroke 27, 927–933 25 Celermajer, D. S., Sorensen, K. E., Bull, C., Robinson, J. and Deanfield, J. E. (1994) Endothelium-dependent dilation in the systemic arteries of asymptomatic subjects relates to coronary risk factors and their interaction. J. Am. Coll. Cardiol. 24, 1468–1474 26 Chambers, J. C., McGregor, A., Jean-Marie, J., Obeid, O. A. and Kooner, J. S. (1999) Demonstration of rapid onset vascular endothelial dysfunction after hyperhomocysteinemia. Circulation 99, 1156–1160 27 Lentz, S. R., Malinow, M. R., Piegors, D. J., BhopatkarTeredesai, M., Faraci, F. M. and Heistad, D. D. (1997) Consequences of hyperhomocyst(e)inemia on vascular function in atherosclerotic monkeys. Arterioscler. Thromb. Vasc. Biol. 17, 2930–2934 28 Bo$ ger, R. H., Sydow, K., Borlak, J. et al. (2000) LDL cholesterol upregulates synthesis of asymmetrical dimethylarginine in human endothelial cells. Involvement of S-adenosylmethionine-dependent methyltransferases. Circ. Res. 87, 99–105 29 Kimoto, M., Sasakawa, T., Tsuji, H. et al. (1997) Cloning and sequencing of cDNA encoding NG,NGdimethylarginine dimethylaminohydrolase from rat kidney. Biochim. Biophys. Acta 1337, 6–10 30 Fickling, S. A., Leone, A. M., Nussey, S. S., Vallance, P. and Whitley, G. S. J. (1993) Synthesis of NG,NGdimethylarginine by human endothelial cells. Endothelium 1, 137–140 31 Bo$ ger, R. H., Bode-Bo$ ger, S. M., Tsao, P. S., Lin, P. S., Chan, J. R. and Cooke, J. P. (2000) An endogenous inhibitor of nitric oxide synthase regulates endothelial adhesiveness for monocytes. J. Am. Coll. Cardiol., in the press 32 MacAllister, R. J., Parry, H., Kimoto, M. et al. (1996) Regulation of nitric oxide synthesis by dimethylarginine dimethylaminohydrolase. Br. J. Pharmacol. 119, 1533–1540 33 Loscalzo, J. (1996) The oxidant stress of hyperhomocyst(e)inemia. J. Clin. Invest. 98, 5–7 34 Upchurch, G. R., Welch, G. N., Fabian, A. J. et al. (1997) Homocyst(e)ine decreases bioavailable nitric oxide by a mechanism involving glutathione peroxidase. J. Biol. Chem. 272, 17012–17017 35 Hanratty, C. G., McAuley, D. F., McGurk, C., Young, I. S. and Johnston, G. D. (1998) Homocysteine and endothelial vascular function (letter). Lancet 351, 1288–1289 36 Niu, X. F., Smith, C. W. and Kubes, P. (1994) Intracellular oxidative stress induced by nitric oxide synthesis inhibition increases endothelial cell adhesion to neutrophils. Circ. Res. 74, 1133–1140 37 Lin, P. S., Bo$ ger, R. H., Bode-Bo$ ger, S. M., Tsao, P. S. and Cooke, J. P. (1997) Asymmetric dimethylarginine enhances superoxide production in human endothelial cells. Circulation 96 (Suppl.), 1–45 38 Xiong, Y., Li, Y. J., Yu, X. J., Liu, G. Z. and Li, N. S. (1996) Endogenous inhibitors of nitric oxide synthesis and lipid peroxidation in hyperlipidemic rabbits. Zhongguo Yaoli Xuebao 17, 149–152

Received 26 June 2000/6 September 2000; accepted 26 October 2000

#

2001 The Biochemical Society and the Medical Research Society

167