lack of ovine or bovine trophoblast cell lines, numerous studies have attempted to define such mechanisms using human choriocarcinoma cells. (e.g. JAR; Cross ...
121
Enhancement of ovine trophoblast interferon by granulocyte macrophage-colony stimulating factor: possible involvement of protein kinase C K Imakawa, K D Carlson1, W J McGuire2, R K Christenson2 and A Taylor1 Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas 66160, USA 1 Department of Biological Sciences, Wichita State University, Wichita, Kansas 67260, USA 2 Reproduction Research Unit, USDA, Agricultural Research Service, Roman L Hruska US Meat Animal Research Center, Clay Center, Nebraska 68933, USA (Requests for offprints should be addressed to K Imakawa, Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, 3901 Rainbow Blvd, Kansas City, Kansas 66160-7410, USA)
ABSTRACT
Interferon-ô (oIFNô), the major secretory product of ovine conceptuses between days 13 and 21 (day 0=day of estrus) of pregnancy, is implicated in the process of maternal recognition of pregnancy. Culturing of day-14 and day-16 conceptus tissues in the presence of human granulocyte macrophagecolony stimulating factor (hGM-CSF) or interleukin3 (IL-3) produces a marked increase in oIFNô mRNA and protein expression. Since GM-CSF and IL-3 are localized at the luminal and glandular epithelia of the ovine endometrium, maternally derived GM-CSF and IL-3 may affect conceptus production of oIFNô in a paracrine manner. However, the molecular mechanisms by which endometrial GM-CSF and IL-3 up-regulate oIFNô production have not been defined. As an initial investigation of the signaling pathway regulating the GM-CSF induction of the oIFNô gene, day-16 conceptuses were treated with an inducer, phorbol 12-myristate 13-acetate (PMA) and an inhibitor, calphostin C of the protein kinase C (PKC) pathway. Treatment with either 150 units/ml hGMCSF (P
