They developed intestinal lesions characterized by swelling, vacuo- lation, and exfoliation .... edema was present in the submucosa and the meso- colon. There were no .... 2 Collins lE, Bergeland ME, Myers LL, Shoop DS: Ex- foliating colitis ...
Veterinary Pathology Online http://vet.sagepub.com/
Enterovirulence of Enterotoxigenic Bacteroides fragilis in Gnotobiotic Pigs J. R. Duimstra, L. L. Myers, J. E. Collins, D. A. Benfield, D. S. Shoop and W. C. Bradbury Vet Pathol 1991 28: 514 DOI: 10.1177/030098589102800608 The online version of this article can be found at: http://vet.sagepub.com/content/28/6/514
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Vet Pathol 28:5 14-5 18 ( 199 1)
Enterovirulence of Enterotoxigenic Bacteroides fragilis in Gnotobiotic Pigs D.
1. R . D U IM ST RA , L. L. MYERS, J. E. COLLINS, A. B EN FIE LD, D . S . SHOO P , AND W. C. B RAD BURY
Department of Veterinary Science, Sout h Dakota State Universi ty, Brooki ngs, SD; College of Veteri nary Medici ne, University of Minnesota, S1. Pau l, MN; Veterinary Molecular Biology Labora tory , Mon tan a State University, Bozeman, MT; Banting Inst itute, Un iversity of Toronto, Toronto, Ontario, Canada Abstract. A porcine isolate of enterotoxigenic Bacteroides fra gilis colonized the intestina l tract and caused watery , nonhemorrhagic diarrhea when given ora lly to 12, 1- to 2-da y-old gnotobiotic pigs. Diarrhea occurred 2 to 3 days post -inoculation and continued thro ugho ut the 4 to 6 day post -inoculation period. Diarrheic pigs beca me mildly anorexic and dehydrated. Th ey dev eloped intestina l lesion s characterized by swelling, vac uolation, and exfoliat ion of enterocytes, and crypt hyperplasia th rougho ut the large intes tine and, to a lesser extent, in the distal small intesti ne. Bacteria l adherence to , or invasion of, the intestinal mucosa was not detected. A porcine isolate of nonenterotoxigenic B.fragilis was adm inist ered orally to six control pigs. The isolate colonized the int estinal tract, but the pigs did not devel op clinic al disease or intestinal lesions. T he pathogenetic mechanism of the diseas e may involve med iat ion by a solub le enterotoxin (or toxins) elaborated by B. fra gilis.
Key words: Bacteroides fragilis; diarrhea; enterotoxin; gnotobiotic pigs; intestinal lesions.
Enterotoxigenic isolates of Bacteroides fragilis have been isolated from the feces of human beings'
