Keywords: Medicinal plant, Satureja bakhtiarica bung, antibacterial, A.baumanni. P251: Determining the Prevalence of Enteroinvasive E. coli (EIEC) Strains in ...
entities. Here, the chemical compositions and antibacterial activity of the essential oils obtained from Satureja bakhtiarica bung against multi drug resistant Acinetobater baumanni isolated from burned patients were evaluated. Methods: Chemical compositions of essential oil were analyzed by gas chromatography mass spectrometry (GC-MS) method. Antibacterial activity of essential oil was evaluated by a well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the macrodilution method Results: The GC-MS spectrums showed 13 compounds, in which the highest chemical composition was related to phenol (37.36%), thymol (22.65%) and p-cymen (19.29%) compounds. The essential oil of Satureja bakhtiarica bung showed good activity against tested bacteria,which is possibly due to the high levels of phenol in their compositions. The MIC and MBC values of A.baumanni sensitive to the essential oil were in the ranges of 3.12 to 6.25 respectively. Conclusion: However, the essential oil of Satureja bakhtiarica bung is a suitable plant drug against multi drug resistant A.baumanni. Keywords: Medicinal plant, Satureja bakhtiarica bung, antibacterial, A.baumanni
P251: Determining the Prevalence of Enteroinvasive E. coli (EIEC) Strains in patients older than 2 Years of age with diarrhea in Shiraz by Real Time PCR technique Pejman Abbasi1, Mohammad Kargar2, Abbas Doosti3, Jalal Mardaneh1, Sadegh Ghorbani-Dalini4, Mohammad Ali Dehyadegari1 1. Professor of Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, 2. 3. 4.
Shiraz, Iran. Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran. Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran. Department of Genetics, University of Sistan and Baluchestan, Zahedan, Iran.
Background and Aim: Enteroinvasive Escherichiacoli (EIEC) cause dysentery; however, it is less widely reported than other etiological agents in studies of diarrhea worldwide. Despite its acknowledged status as a human pathogen, very little research has been conducted to identify individual risk factors for infection, possible reservoirs, or even infection rate. The objective of this study was to evaluate the real-time PCR as a rapid diagnostic tool for detection of four categories of EIEC in adult patients with diarrhea in Shiraz. Methods: A total of 430 stool samples were collected from patients with diarrhea in Shiraz, in 2012. Diarrheagenic E. coli (DEC) strains were isolated by standard biochemical analysis. We used Real time PCR and melt curve analysis to detect the presence of ipaH gene in EIEC. Results: 430 stool samples were tested in which 52 (12.09%) were identified as contaminated with E. coli by biochemical and microbiology tests. In the present study, EIEC were detected in 5 adult patients with diarrhea.
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Conclusion: The results of this study showed that Real-time PCR offers the advantage of being a faster, more robust assay because it does not require post-PCR procedures to detect amplification products. Keywords: Prevalence, Enteroinvasive Escherichia coli (EIEC), Real-Time PCR, Shiraz.
P252: Immunological Evaluation of Chitosan nanoparticles containing E. coli O157:H7 Detoxified Lipopolysaccharide Conjugated to Recombinant Exotoxin APseudomonase aeroginosain Mouse Model with amidation me Somayeh Afshari1, Esmaeil Dehestani2 1. 2.
Department of microbiology, Islamic azad university, Zanjan, Iran. Department of microbiology, Islamic azad university, Zanjan, Iran.
Background and Aim: Patients with infection of E.coli O157:H7 have shown bloody diarrhea, a dangerous and threatening complication called hemolytic syndrome that is uremic and cause dead in under 5 years old childrens.Lipopolysaccharide (LPS) of this bacteria induce a strong response in immune system of host and therefore candidate target for new generation of conjugate vaccines. Among biodegradable polymers, chitosan polymer has great features to increase the response of immunity system.The aim of present study was to determine immunological evaluation of chitosan nanoparticle containing E. coli O157:H7 detoxified LPSRecombinant ExotoxinA Pseudomonas aeroginosa (D-LPS-rEPA) conjugate in mice as a candidate vaccine. Methods: After cultivation of bacteria, LPS was extracted with modified hot phenol method, dialysis, concentration, and electrophoresis and detoxified were done. To improve immunogenicity, the detoxified LPS was coupled to tetanus toxoid with ADH as a spacer and EDAC as a linker and this conjugate purified by CL2B sepharose column of gel filtration.Chitosan nanoparticles were prepared by Isooctane emulsion in the organic phase. Pyrogenicity tests showed that resulting conjugate was non-pyrogenic. Then four groups of female BALB/c mice (each group was included 15 mice) was selected. Vaccination was performed by three doses with two week interval. Then serum samples were collected and antibodies response against LPS was measured by ELISA method for total IgG, IgM, IgA. Results: Two weeks after first dose there was no significance different between antibodies titers in groups that were immunized with D-LPS and D-LPS-rec Exo A. But after second and third doses,D- LPS-rec Exo A showed significance increasing in all types of antibodies titers concentration against LPS in versus D-LPS.The results of anti-LPS inductions for total IgG, IgM, IgA were observed D-LPS-rec Exo A>D-LPS>rec Exo A Conclusion: These results indicated that LPS from E. coli O157:H7 increases anti-LPS antibodies in conjugate form with Recombinant ExotoxinA pseudomonas aerojinosa and can be appropriate effective conjugate. Keywords: E.coli O157:H7, Conjugation,Chitosan, Recombinant ExotoxinA, pseudomonas aerojinosa.
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