RESEARCH ARTICLE
Enzymatically Modified Starch Favorably Modulated Intestinal Transit Time and Hindgut Fermentation in Growing Pigs M. A. Newman1,2, Q. Zebeli1,2, K. Velde3, D. Gru¨ll4, T. Molnar4, W. Kandler5, B. U. MetzlerZebeli2,6*
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1 Institute of Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine Vienna, Vienna, Austria, 2 Research Cluster “Animal Gut Health”, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria, 3 Equine University Clinic, Department for Companion Animals and Horses, University of Veterinary Medicine, Vienna, Austria, 4 Agrana Research & Innovation Center GmbH, Tulln, Austria, 5 Center for Analytical Chemistry, Department of Agrobiotechnology, IFA-Tulln, University of Natural Resources and Life Sciences, Vienna, Austria, 6 University Clinic for Swine, University of Veterinary Medicine, Vienna, Austria *
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Abstract OPEN ACCESS Citation: Newman MA, Zebeli Q, Velde K, Gru¨ll D, Molnar T, Kandler W, et al. (2016) Enzymatically Modified Starch Favorably Modulated Intestinal Transit Time and Hindgut Fermentation in Growing Pigs. PLoS ONE 11(12): e0167784. doi:10.1371/ journal.pone.0167784 Editor: Franc¸ois Blachier, National Institute for Agronomic Research, FRANCE Received: October 4, 2016 Accepted: November 21, 2016 Published: December 9, 2016 Copyright: © 2016 Newman et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper. Funding: This project was funded by the Austrian Research Promotion Agency (FFG), BRIDGE programme (no. 836447-“Healthy Carbohydrates”), https://www.ffg.at/en/bridge. Agrana Research & Innovation Center GmbH provided support in the form of salaries for authors [DG, TM], but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the
Aside from being used as stabilizing agents in many processed foods, chemically modified starches may act as functional dietary ingredients. Therefore, development of chemically modified starches that are less digestible in the upper intestinal segments and promote fermentation in the hindgut receives considerable attention. This study aimed to investigate the impact of an enzymatically modified starch (EMS) on nutrient flow, passage rate, and bacterial activity at ileal and post-ileal level. Eight ileal-cannulated growing pigs were fed 2 diets containing 72% purified starch (EMS or waxy cornstarch as control) in a cross-over design for 10 d, followed by a 4-d collection of feces and 2-d collection of ileal digesta. On d 17, solid and liquid phase markers were added to the diet to determine ileal digesta flow for 8 h after feeding. Reduced small intestinal digestion after the consumption of the EMS diet was indicated by a 10%-increase in ileal flow and fecal excretion of dry matter and energy compared to the control diet (P
