Seventeen strains of Propionibacterium acnes and ten strains of P. granulosum were isolated from acne patients, in ad- dition to ten isolates of each species ...
Qatar Univ. Sci. J. (1996), 16(2): 275-278
ENZYME PRODUCTION AND ANTIBIOTIC SUSCEPTIBILITY OF PROPIONIBACTERIUM ACNES AND P. GRANULOSUM FROM ACNE VULGARIS PATIENTS AND HEALTHY PERSONS
AL-HADITHI, H. T.*, and KHUDAIER, B. Y.** *Assistant Prof., Biology Dept., College of Science, Univ. Basrah, Iraq **Assistant Lect., Microbiology Dept., College of Veterinary Medicine, Univ. Bashra, Iraq
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ABSTRACT Seventeen strains of Propionibacterium acnes and ten strains of P. granulosum were isolated from acne patients, in addition to ten isolates of each species from healthy persons. All strains were examined for their enzymatic activity and antibiotic susceptibility to ten antibiotics. Percentage of lipase, lecithinase and casenase production by strains of both species from acne lesions was higher than that from healthy persons in contrast to gelatinase. Haemolysin was produced almost by all strains. All isolates were resistant to ampicillin, penicillin and tetracycline. Strains of both species from healthy persons were more susceptible to the remaining antibiotics than those from acne lesions with significant differences at various levels.
INTRODUCTION The relevance of Propionibacterium in the complex etiology of acne has been widely discussed. Some investigators demonstrated differences between acne patients and healthy persons concerning number, regional variations, species and types of Propionibacterium (1, 2, 3, 4). Their role in the genesis of fatty acids has also been reported (5, 6, 7, 8). The effect of glucose concentration, oxygen tension and pH on growth rate and extracellular enzyme production was also examined (9, 10, 11). Extracellular enzymes may function to supply the organism
with carbon/energy sources in vivo (7, 12). Hoeffler et al. (13) were the first to study the difference in the production of extracellular enzymes between propionibacteria strains isolated from acne patients and healthy persons. However, different species of Propionibacterium were not considered in their study. The aim of this study was to determine and compare the ability of two species of Propionibacterium: P. acnes and P. granulosum to produce five extracellular enzymes and the susceptibility of both species towards ten antibiotics. Strains of the two species were isolated from acne patients and healthy persons.
Enzyme Production and Antibiotic Susceptibility of Propionibacterium
significantly higher than that of H strains (P
