(Ephemeroptera: Baetidae) from Southeastern Brazil

Zootaxa 3526: 17–30 (2012) www.mapress.com / zootaxa/ Copyright © 2012 · Magnolia Press

ISSN 1175-5326 (print edition)

Article

ZOOTAXA ISSN 1175-5334 (online edition)

urn:lsid:zoobank.org:pub:9BB67C73-5090-42B1-906D-84A3E05296A7

New species and new records of Camelobaetidius Demoulin, 1966 (Ephemeroptera: Baetidae) from Southeastern Brazil R. BOLDRINI1,5, A.M.O. PES2, C.N. FRANCISCHETTI3 & F.F. SALLES4 1

Divisão de Curso de Entomologia (DCEN), Instituto Nacional de Pesquisas da Amazônia (INPA), CEP 69060-001, Manaus, AM, Brazil. E-mail: [email protected] 2 Coordenação de pesquisa em Entomologia (CPEN), Instituto Nacional de Pesquisas da Amazônia (INPA), CEP 69060-001, Manaus, AM, Brazil. E-mail: [email protected] 3 Núcleo Descentralizado de Vigilância em Saúde da Região Metropolitana 1, Superintendência de Vigilância em Saúde, Secretaria Estadual de Saúde do Rio de Janeiro, Nova Iguaçu, RJ, Brazil, E-mail: [email protected] 4 Laboratório de Sistemática e Ecologia de Insetos, Depto. de Ciências Agrárias e Biológicas, Universidade Federal do Espírito Santo, CEP 29.933-415, São Mateus, ES, Brazil. E-mail: [email protected] 5 Corresponding Author. [email protected]

Abstract The genus Camelobaetidius Demoulin, 1966 has 37 species distributed throughout the Nearctic and Neotropical Regions, being one of the most studied genera of the family Baetidae. The nymphs are common in lotic habitats, can often be found on top of rocks, and can be easily recognized by the presence of the spatulate tarsal claws. Based on material from Southeastern Brazil, the aim of the present paper is to describe two new species with the terminal filament as long as the cerci (C. juparana sp. nov. and C. spinosus sp. nov.), to describe the female of C. anubis (Traver & Edmunds, 1968), to describe new characters for the male of this species, and to present new records for the genus in southeastern Brazil. Key words: Taxonomy, Macroinvertebrate, South America, Neotropics

Introduction The Southeastern Region of Brazil, represented by the states of Rio de Janeiro, Espírito Santo, Minas Gerais, and São Paulo, is one of the most studied areas of the country regarding mayflies (Ephemeroptera) (Salles et al. 2004a). Despite this fact, several species or even genera have yet to be described from the region, notably from the families Baetidae, Leptophlebiidae and Leptohyphidae. After examination of several nymphs of Baetidae from this area, as a part of the project “Baetidae (Insecta: Ephemeroptera) from Southeastern Brazil” two new species of the genus Camelobaetidius Demoulin, 1966 have been found. This genus is comprised currently of 37 species, with 33 species based on nymphs and four species based on adults (Lugo-Ortiz & McCafferty, 1999; Jacobus & McCafferty, 2005; Salles & Serrão, 2005; Domínguez et al., 2006; Boldrini & Salles, 2009; Salles & Nascimento, 2009), and it is distributed from Argentina (Traver & Edmunds, 1968) to Canada (Lehmkuhl, 1976). The genus achieves its greatest diversity in Brazil, where 18 species have been reported until now (Salles & Serrão, 2005; Boldrini & Salles, 2009; Falcão et al., 2011). Besides the description of the new species, the goals of the present paper are to describe the female imago of C. anubis (Traver & Edmunds, 1968), to add new characters to the description of the male imago of this species, and to provide new records for two species from Southeastern Brazil.

Material and methods Pictures were taken using a Leica (M165C) stereomicroscope with a DFC420 digital camera; a series of partially Accepted by L.M. Jacobus: 9 Oct. 2012; published: 25 Oct. 2012

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focused images were processed with the program Leica Application Suite V3.4.1. (Version 2009) to produce final images with enhanced quality. Line drawings were made with the aid of a camera lucida, photographs, or both. All final illustrations were prepared according to Coleman (2003, 2006). The identifications of previously described species of Camelobaetidius were based on the keys of Salles & Serrão (2005) and Domínguez et al. (2006), and with the original descriptions or pertinent redescriptions of each species. The slides were prepared with Euparal®. Terms used in descriptions of the adult thorax are from Kluge (1994). The material examined is housed in the following institutions: Invertebrate Collection of the Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus, Brazil, and Zoological Collection Norte Capixaba, (CZNC), São Mateus, Brazil.

Results

Camelobaetidius anubis (Traver & Edmunds, 1968) (Figs. 1–8) Dactylobaetis anubis Traver & Edmunds, 1968: 666. Camelobaetidius anubis McCafferty & Waltz, 1990: 783; McCafferty & Klubertanz, 1994: 41; Lugo-Ortiz & McCafferty, 1995: 187; Dominique & Thomas, 2002: 32; Nieto, 2003: 234; Thomas et al., 2003b: 130; Salles et al., 2003: 5; Salles & Dias, 2004: 269; Salles et al., 2004a: 727; Francischetti et al., 2004: 35; Salles et al., 2004c: 15; Salles & Serrão, 2005: 268; Domínguez et al., 2006: 127; Boldrini & Salles, 2009: 10; Salles et al., 2010: 302; Falcão et al., (2011): 252.

Diagnoses. Nymph: 1) Segment II of labial palp with distomedial projection triangular with apex pointed; 2) abdominal terga orange brown with reddish brown marks on segments III and VI (Fig. 7); 3) thoracic gill present at base of forecoxa; 4) tarsal claws with 20–27 denticles; 5) paraproct with 3 pointed marginal spines; 6) terminal filament as long as cerci. Male adult: 1) Turbinate portion of compound eyes orange (Figs. 1, 2); 2) anteronotal protuberance pointed (Fig. 1); 3) abdominal terga orange brown with medial reddish brown marks on segments III and VI (Fig. 2 ); 4) posterior margin of sugenital plate convex, posteriorly projected; 5) forceps segment I without distomedial projection; 6) forceps segment I 0.3× length of segment II; forceps segment III 0.2× length of segment II (Fig. 3). Female adult: 1) anteronotal protuberance pointed (as in Fig. 1); 2) abdominal terga orange brown with medial reddish brown marks on segments III and VI (Fig. 4). Comments. This species was described based on nymphs from Brazil, states of Paraná and Santa Catarina (Traver & Edmunds, 1968). Nieto (2003) recorded this species from Argentina and described the male imago. Later, Salles et al. (2003, 2004a), Francischetti et al. (2004) and Boldrini & Salles (2009) recorded this species from Southeastern Brazil. Falcão et al. (2011) have also recorded this species from Northern Brazil, although some important variations were found in the nymphs (i.e. 35 denticles on tarsal claw). Herein, we improve the description of the male adult of C. anubis proposed by Nieto (2003), by adding new characters, and describe the female imago of this species. Male adult. Length of tibia I: 1.6 mm; tibia II: 1.2 mm, and tibia III: 1.0 mm. Head (Figs. 1, 2). Dorsal portion of turbinate eyes oval; length 1.6× width; stalk height 0.5× width of dorsal portion; inner margins parallel, not reaching each other. Thorax (Figs. 1, 2). Metanotum with anteronotal protuberance pointed; metascutellar protuberance rounded and of the same level of the metanotum; pleura yellowish. Tarsi segment II of the middle and hind legs with one short apical spine (as in Fig. 7). Leg I: tibia 2.1× length of femur; tarsi 1.2× length of femur. Leg II: tibia 1.7× length of femur; tarsi 0.3× length of femur. Leg III: tibia 1.5× length of femur; tarsi broken. Abdomen (Figs. 1, 2). Terga orange brown with medial reddish brown marks on segments III and VI. Genitalia (Fig. 3). Forceps segment I without distomedial projection, 0.3× length of segment II; distance between inner base of forceps 0.6× distance between lateral margins of forceps. Forceps segment II narrow submedially. Forceps segment III elongate, 2.9× as long as wide; 0.2× length of segment II. Posterior margin of subgenital plate convex, posteriorly projected.

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BOLDRINI

FIGUES 1–7. Camelobaetidius anubis, adults. 1–3, male. 4–7, female. 1, body (l. v.). 2, body (d. v.). 3, genitalia. 4, body (d. v.). 5, forewing. 6a, hind wing. 6b, hind wing (enlarged). 7, detail of tarsal claw.

NEW CAMELOBAETIDIUS FROM BRAZIL

Zootaxa 3526 © 2012 Magnolia Press ·

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FIGUES 8–12. Camelobaetidius, nymphs (d. v.). 8, C. anubis. 9, C. cayumba. 10, C. janae. 11, C. juparana sp. nov. 12, C. spinosus sp. nov.


BOLDRINI

Female adult. Length of body: 5.2–5.8 mm; cerci: broken; forewing: 5.3–5.5 mm; hind wing: 0.9 mm; tibia I: 1.1 mm; tibia II: 1.2 mm; tibia III: 0.7 mm. Head (Fig. 4). Yellowish brown, with whitish marks on vertex and base of compound eyes; ocelli whitish with base darker; antenna yellowish brown. Thorax (Fig. 4). Pro, meso and metanotum yellowish brown. Pronotum with lateral margin dark brown; mesonotum with medioparapsidal suture dark brown, anteronotal protuberance pointed (as in Fig. 1); metanotum with posterior margin dark brown, metascutellar protuberance rounded and at the same level of metanotum; pleura yellowish. Legs. Tarsi segment II of legs with one short apical spine (Fig. 7). Femur yellowish brown; tibia and tarsi brown. Leg I: tibia 1.3× length of femur; tarsi 0.5× length of femur; segments IV>I>II>III. Leg II: tibia 1.4× length of femur; tarsi 0.3× length of femur. Leg III: tibia 1.1× length of femur; tarsi 0.2× length of femur. Forewing membrane hyaline (Fig. 5); veins light brown; stigmatic area with six cross veins; marginal intercalary veins paired, except between veins ICu2 and A; length of forewing about 2.5× width. Hind wing membrane and veins hyaline (Figs. 6a, 6b); with two complete longitudinal veins, without cross veins; costal process pointed, located on basal third. Abdomen (Fig. 4). Terga orange brown with medial reddish brown marks on segments III and VI, and brown marks present on lateral margin of the segments II, III and V. Sterna yellowish white. Distribution: ARGENTINA; BRAZIL: states of Espírito Santo, Minas Gerais, Paraná, Rio de Janeiro, Roraima, Santa Catarina and São Paulo. . Material examined. Two male imagos (reared), BRAZIL, Espírito Santo, Alfredo Chaves, Nova Mantova, 20º39’22.6’’S/ 40º50’12.9’’W, 5.xii.2010, Boldrini, R. (CZNC). Same as preceeding except one female imago (reared), Boldrini, R. (INPA). Same as preceeding except one female imago,12.xiii.2007, Boldrini, R. (CNZC).

Camelobaetidius cayumba (Traver & Edmunds, 1968) (Fig. 9) Dactylobaetis cayumba Traver & Edmunds, 1968: 669. Camelobaetidius cayumba McCafferty & Davis, 1992: 207; Dominique et al., 2002a: 18; Dominique et al., 2002b: 39; Domínguez et al., 2002: 462; Thomas et al., 2003a: 118; Thomas et al., 2003b: 130; Salles & Serrão, 2005: 269; Domínguez et al., 2006: 128; Dias et al., 2009: 238; Falcão et al., 2011: 527; Boldrini et al., 2012: 93; Lima et al. 2012: 63.

Diagnoses. Nymphs: 1) Segment II of labial palp with distomedial process triangular with apex pointed; 2) thoracic gill absent at the base of forecoxa; 3) tarsal claws with 15–18 denticles; 4) paraproct with 3 pointed marginal spines; 5) terminal filament as long as cerci. Male adult: 1) anteronotal and metascutellar protuberance rounded; 2) terga of abdominal segments II –III and VI medially with remarkable dark marks; 3) forceps segment I without distomedial projection; 4) posterior margin of subgenital plate convex, posteriorly projected. Female: 1) anteronotal and metascutellar protuberance rounded; 2) abdominal terga with segments I–II and V medially with dark spots. Comments. This species was described based on nymphs from Peru (Traver & Edmunds, 1968). Thomas et al. (2003a) subsequently redescribed this species based on nymphs from French Guiana. Domínguez et al. (2002) recorded this species from Colombia. Salles & Serrão (2005) recorded it for the first time from Brazil (states of Goiás and Rondônia); Falcão et al. (2011) recorded this species from the State of Roraima; Boldrini et al. (2012) recorded this species from States of Piauí, Maranhão and Ceará; and Lima et al. (2012) described the adults from state of Pernambuco (Brazil). Distribution. COLOMBIA; FRENCH GUIANA; PERU; BRAZIL: states of Ceará, Goiás, Maranhão, Pernambuco, Piauí, Rondônia and Roraima. NEW RECORD: Espírito Santo. Material examined. Two nymphs mounted on slides, BRAZIL, Espírito Santo, Sooretama, Rio São José, 19°07'33.1"S/ 40°14'26.1"W, 4.x.2010, 24 m, Boldrini, R., Salles, F.F. & Nascimento, J.M.C. leg (CZNC).



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FIGUES 13–18. Camelobaetidius juparana sp. nov., nymph. 13, labrum (left v.v., right d.v.). 14, left mandible. 15, right mandible. 16, hypopharynx. 17, maxilla. 18a, labium (left v.v., right d.v.). 18b, detail of setae of paraglossa.


BOLDRINI

Camelobaetidius janae Dominique & Thomas, 2001 (Fig. 10) Camelobaetidius janae Dominique et al., 2001a: 44; Dominique et al., 2001b: 97; Dominique & Thomas 2002: 27; Dominique et al., 2002b: 39; Thomas et al., 2003b: 130; Salles et al., 2004b: 5; Salles et al., 2004c: 15; Salles & Serrão, 2005: 275; Domínguez et al., 2006: 131; Falcão et al., 2011: 528; Nieto et al., 2011: 8; Boldrini et al., 2012: 93.

Diagnoses. Nymphs: 1) Segment II of labial palp with distomedial projection rounded and strongly projected; 2) maxillary palp segment II bifid; 3) thoracic gill absent at base of forecoxa; 4) tarsal claws with 8–21 denticles; 5) paraproct with 4 pointed marginal spines; 6) terminal filament as long as cerci. Comments. This species was described based on nymphs from French Guyana (Dominique et al., 2001a). Salles et al. (2004b) recorded it for the first time from Brazil from the state of Mato Grosso. Later, this species was recorded from the states of Rondônia (Salles et al., 2005), Roraima (Falcão et al. 2011) and Piauí (Boldrini et al., 2012). Nieto et al. (2011) recorded this species for the first time from Venezuela. As in Falcão et al. (2011), we found differences in relation to the number of denticles on the tarsal claws as originally described by Dominique et al. (2001), with 21 denticles on tarsal claws. Falcão et al. (2011) found one nymph with 8–9 denticles, and we have also found the same variation in two nymphs in the material from states of Minas Gerais and Espírito Santo. As in Falcão et al. (2011), we are considering this as an intra-specific variation. Distribution. FRENCH GUIANA; VENEZUELA; BRAZIL: states of Mato Grosso, Piauí, Rondônia and Roraima. NEW RECORDS: Espírito Santo and Minas Gerais. Material examined. Three nymphs mounted on slides, BRAZIL, Minas Gerais, Pingo da Água, Rio Sacramento, 19º49’S/ 42º18’W, 29.x.2005, Francischetti, C. N. & Salles, F.F. leg (CZNC). One nymph, BRAZIL, Espírito Santo, Sooretama, Rio São José, 19°07’33.1’’S/ 40°14’26.1’’, 23.i.2012, W, Nascimento, J.M.C. leg. (CZNC).

Camelobaetidius juparana sp. nov. Boldrini & Salles (Figs. 11; 13–22) Diagnoses. Nymphs: 1) Segment II of labial palp with distomedial projection robust and rounded (Fig. 18); 2) bifid thoracic gill present at the base of forecoxa (Fig. 19a); 3) tarsal claws with 23–29 denticles (Fig. 19b); 4) paraproct without spines (Fig. 22); 5) terminal filament as long as cerci. Comments. Camelobaetidius juparana sp. nov. is possibly closely related to C. hamadae Salles & Serrão, 2005 another species in which the nymphs present a bifid thoracic gill at the base of the forecoxa and a paraproct without spines. It is easily distinguished from C. hamadae by the number of setae on the dorsal margin of labrum, 10 versus 32 in C. hamadae; by the shape of the hypopharynx, superlingua with distal margin oblique versus truncated in C. hamadae, and lingua subequal in length to superlingua versus superlingua shorter than lingua in C. hamadae; by the number of denticles on tarsal claws, 23–29 denticles versus 36–37 denticles in C. hamadae; and by the shape of the spines on posterior margin of terga IV: it is truncate in C. juparana sp. nov., and in C. hamadae this spines are triangular. Description Nymph. Length of body: 2.9–3.5 mm; length of antennae: 1.3 mm; length of cerci: 1.7 mm; length of terminal filament: 1.4 mm; tibia I: 0.5 mm; tibia II: 0.5 mm; tibia III: 0.4 mm. Head (Fig. 11). Light brown. Antennae light brown, with scape and pedicel subcilindrical. Lateral branch of epicranial suture straight. Labrum (Fig. 13). Subrectangular, broader than long. Length about 0.6× maximum width; lateral margin with long, fine and simple setae; anterolateral margin with long, fine and pectinate setae; medially with short, robust, pectinate setae. Dorsally with seven shorts, fine, simple setae scattered over basal area; dorsolateral arc of setae with a row 10 long, fine and apically pointed setae. Left mandible (Fig. 14). Prostheca robust, apically denticulate. Margin between prostheca and mola straight with a row of spine-like setae; subtriangular process narrow and produced; tuft of setae at apex of mola absent. Basal half bare.



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Right mandible (Fig. 15). Prostheca slender, apically denticulate; margin between prostheca and mola straight, with two spine-like setae; tuft of spine-like setae at base of mola present; tuft of setae at apex of mola present. Basal half bare. Hypopharynx (Fig. 16). Lingua subequal in length to superlingua; apex rounded, with tuft of short, fine, simple setae. Superlingua not expanded with fine, apex truncated obliquely, simple setae scattered over lateral and distal margin. Maxilla (Fig. 17). Crown of galea-lacinia with four denticles; inner dorsal row of setae with two denti-setae. Medial protuberance of galea with 1 + 2 long, fine, simple setae. Maxillary palp 1.3× length of galea-lacinia. Labium (Fig. 18a). Glossa narrow and subequal in length to paraglossa; inner margin with six spine-like setae increasing in length distally; apex with one long, spine-like setae; outer margin with three long, spine-like setae; ventral surface with one short, fine, simple setae on anteromedian area. Paraglossa sub-rectangular, curved inward; apex with two rows of long, fine, pectinate setae (Fig. 18b); ventral surface bare; dorsal surface with three robust, simple setae near apex. Labial palp with segment I subequal in length of segments II and III combined; outer margin of segment I with short, simple setae near apex; segment II with distomedial protuberance robust and rounded, with simple setae over lateral and ventral area; dorsally with a row of three fine, spine-like setae; segment III rounded, length 0.7× width, scattered with fine, simple setae, and fine, spine-like setae. Thorax (Fig. 11). Light brown with marks brown; metanotum light brown washed with brown. Hind wing pad present. Bifid thoracic gill (Fig. 19a) present at the base of forecoxa. Femur light brown with a medial brown mark, and with dorsal surface and apex brown; tibia light brown with base and apex brown, tarsi light brown washed with brown. Tibia I, II and III subequal in length of the respective femur; tarsi I about 0.7× in length of femur; tarsi II and III about 0.5× in length of the respective femur. Forefemur (Fig. 19a). Length about 2.2× maximum width; dorsally with row of c.a. 40 long, spine-like setae; length of setae 0.3× width of femur; ventrally with short, spine-like setae. Foretibia (Fig. 19a). Dorsally with row of short, fine, simple setae; ventrally with few, short, spine-like setae; anterior surface with a row of long, fine, simple setae; tibio-patelar suture present, 0.6× in length of tibia. Foretarsi (Fig. 19b). Dorsally and ventrally with short, fine, simple setae; ventrally with five spine-like setae, increasing in length toward apex, and one long, fine, simple setae near apex. Tarsal claws (Fig. 19b) with 23–29 denticles. Abdomen (Fig. 11). Light brown; segments I, III and VI washed with brown, and anterior margin of the segment VII washed with brown; segments III and VI with distinct median and lateral brown marks. Terga IV with surface creased (Fig. 20); posterior margin with truncate spines. Gills IV (Fig. 21a). Light brown; margins with narrow spine intercalating short, fine, simple setae; tracheae pigmented (Fig. 21b), extended from main trunk. Gills oval. Gill I 0.5× length of segment II; gill IV 2.1× length of segment V; gill VII 0.9× length of segment VIII. Paraproct (Fig. 22). Without spines near on inner margin; surface with fine, simple setae, without shagreened area; postero-lateral extension with rounded marginal spines. Cerci light brown; outer and inner margin of the terminal filament and inner margin of the cerci with tufts of long, simple setae. Etymology: The specific epithet juparana is a reference to Lagoa Juparanã, one of Brazilian largest lakes. The São José River, where the species has been found, is the main tributary of Lagoa Juparanã. Distribution. BRAZIL: Espírito Santo, Minas Gerais. Material examined. Holotype: One nymph, BRAZIL, Espírito Santo, Sooretama, Rio São José, 19°07'33.1"S/ 40°14'261"W, 4.x.2010, 24 m, Boldrini, R., Salles, F.F. & Nascimento, J.M.C. leg (CZNC). Paratypes: One nymph mounted in slides, same data holotype (CNZC). One nymph, same data holotype (CNZC). One nymph, same data holotype (INPA).Three nymphs mounted in slides, same data holotype, except 9.ix.2010 (INPA). One nymph mounted on slides, Minas Gerais, Pingo D´Agua, Rio Sacramento, 19º49’S/ 42º18’W, 29.x.2005, Francischetti, C.N. & Salles, F.F. leg (CZNC).

Camelobaetidius spinosus sp. nov. Boldrini & Salles (Figs. 12; 23–32) Diagnoses. Nymphs: 1) Segment II of labial palp with distomedial projection triangular with apex pointed (Fig. 28 ); 2) thoracic gill absent at the base of forecoxa; 3) tarsal claws with 20–24 denticles (Fig. 29); 4) posterior margin of terga with pointed spines (Fig. 30); 5) paraproct with six pointed spines (Fig. 32); 6) terminal filament as long as cerci.


BOLDRINI

FIGUES 19–22. Camelobaetidius juparana sp. nov., nymph. 19a, foreleg. 19b, detail tarsal claw. 20, posterior margin of terga IV. 21a, gill IV. 21b, detail of margin of the gill IV. 22, paraproct.



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FIGUES 23–28. Camelobaetidius spinosus sp. nov., nymph. 23, labrum (left v.v., right d.v.). 24, left mandible. 25, right mandible. 26, hypopharynx. 27, maxilla. 28, Labium (left v.v., right d.v.).


BOLDRINI

FIGUES 29–32. Camelobaetidius spinosus sp. nov., nymph. 29, foreleg. 30, posterior margin of terga IV. 31a, gill IV. 31b, detail of margin of the gill IV. 32, paraproct.



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Comments. Camelobaetidius spinosus sp. nov. is possibly closely related to C. cayumba, both species present the unique combination of characteristics such as the terminal filament as long as cerci, segment II of labial palp with distomedial projection triangular with apex pointed, and thoracic gill absent at base of forecoxa. This species is easily distinguished from C. cayumba by the presence of pointed spines on posterior margin of terga. Description Nymph. Length of body: 3.4–4.0 mm; length of antennae: 1.6–1.7 mm; length of cerci: 2.2 mm; length of terminal filament: 2.2 mm; tibia I and II: 0.8 mm; tibia III: 0.7 mm. Head (Fig. 12). Light brown. Antennae light brown, scape and pedicel subcilindrical. Lateral branch of epicranial suture straight. Labrum (Fig. 23). Narrowly rounded anteriorly, base wider than apex, broader than long. Length about 0.6× maximum width; lateral margin with long, fine, simple setae; anterolateral margin with long, fine, pectinate setae; medially with short, robust, apically pectinate setae. Dorsally with four short, fine, simple setae scattered over basal area; dorsolateral arc of setae with a row 1 + 8 long, fine, apically pointed setae. Ventrally with two short, spinelike setae on anterolateral margin. Left mandible (Fig. 24). Prostheca robust, apically denticulate. Margin between prostheca and mola straight, without a row of spine-like setae; subtriangular process narrow and produced; tuft of setae at apex of mola absent. Basal half bare. Right mandible (Fig. 25). Prostheca robust, shorter than left mandible, apically denticulate; margin between prostheca and mola almost straight, with a row of spine-like setae; tuft of spine-like setae at base of mola present; tuft of setae at apex of mola present. Basal half bare. Hypopharynx (Fig. 26). Lingua subequal in length to superlingua; apex rounded, with tuft of short, fine, simple setae. Superlingua not expanded with fine, simple setae scattered over lateral and distal margin. Maxilla (Fig. 27). Crown of galea-lacinia with four denticles. Inner dorsal row of setae with two pectinate denti-setae. Medial protuberance of galea with 1 + 3 long, fine and simple setae. Maxillary palp 1.4× in length of galea-lacinia. Palp segment II 0.6× in length of segment I. Fine and simple setae scattered over maxillary palp. Labium (Fig. 28). Glossa narrow and shorter than paraglossa; inner margin with ten spine-like setae increasing in length distally; apex with two long, spine-like setae; outer margin with four long, spine-like setae; ventral surface with six short, fine, simple setae on anteromedian area; dorsal surface bare. Paraglossa sub-rectangular, curved inward; apex with two rows of long, fine, simple setae; ventral surface bare; dorsal surface with three fine, spine-like setae near to apex. Labial palp with segment I subequal in length of segments II and III combined; segment II with distomedial projection triangular with apex pointed; dorsally with a row of three fine, spine-like setae; segment III rounded, length 0.6× width, scattered with simple setae, and spine-like setae. Thorax (Fig. 12). Light brown; metanotum brown. Hind wing pad present. Thoracic gill absent at the base of forecoxa. Femur, tibia and tarsi light brown; apex of femur brown. Tibia I and II 0.8× in length of the respective femur; tibia III 0.9× in length of femur III; tarsi I, II and III about 0.4× in length of the respective femur. Forefemur (Fig. 29). Length about 3.6× maximum width; dorsally with row of c.a. 65 long, spine-like setae; length of setae 0.2× maximum width of femur; ventrally with seven short, spine-like setae. Foretibia (Fig. 29). Ventrally with row of six short, spine-like setae; anterior surface with a row of long, fine, simple setae; tibiopatelar suture less than half the length of tibia. Foretarsi (Fig. 29). Ventrally with a row of five spine-like setae increasing in length toward apex. Tarsal claws with 20–24 denticles. Abdomen (Fig. 12). Light brown. Terga IV with surface creased (Fig. 30); posterior margin with pointed spines. Gills IV (Fig. 31a). Light brown; margins with narrow spine intercalating short, fine, simple setae (Fig. 31b); tracheae pigmented, extended from main trunk. Gills oval. Gill I 0.9× length of segment II; gill IV 1.7× length of segment V. Gill VII 0.7× length of segment VIII. Paraproct (Fig. 32). with six spines near inner margin; surface with fine, simple setae, without shagreened area; postero-lateral extension with minute marginal spines. Cerci light brown and terminal filament whitish, distal 3/4 with a brown mark; outer and inner margin of the terminal filament and inner margin of the cerci with tufts of long, simple setae. Etymology: From the Latin word spin, due to the spines on the posterior margin of the terga of the nymph. Distribution. BRAZIL: Minas Gerais. Material examined. Holotype: One nymph, BRAZIL, Minas Gerais, Bom Jesus do Galho, 19º43’669’’S/ 42º28’200’’W, 23.vii.2005, Francischetti, C.N. leg (CZNC). Paratypes: One imature nymph, same data holotype


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(INPA). One nymph, BRAZIL, Minas Gerais, Bom Jesus do Galho, 19º43’669’’S/ 42º28’200’’W, 29.x.2005, Francischetti, C.N. and Salles, F.F. leg, without mouth parts (CZNC). Same as preceeding except one nymph mounted on slides, 23.iv.2005, Francischetti, C.N. (CZNC).One nymph mounted on slides, Minas Gerais, Pingo D´Agua, Rio Sacramento, 19º49’S/ 42º18’W, 29.x.2005, Francischetti, C.N. & Salles, F.F. leg (INPA).

Acknowledgements We would like to express our gratitude to FAPES (Fundação de Apoio à Ciência e Tecnologia do Espírito Santo, process number 3632763/07, 45429502/09), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) and CAPES Pro-Equipamentos (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for financial support. The authors would like to thank Dr. Neusa Hamada (Instituto Nacional de Pesquisas da Amazônia) for logistic support.

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