Abstract- The study was conducted with an aim of developing a genotype independent efficient in vitro regeneration system for Canola (Brassica napus L.) that is ...
Global Journal of Science Frontier Research Biological Science Volume 13 Issue 7 Version 1.0 Year 2013 Type : Double Blind Peer Reviewed International Research Journal Publisher: Global Journals Inc. (USA) Online ISSN: 2249-4626 & Print ISSN: 0975-5896
Establishment of Regeneration Protocol for Canola (Brassica napus L.) By Sabrina Shameen Alam, Laila Khaleda & Mohammad Al-Forkan University of Chittagong, Bangladesh
Abstract- The study was conducted with an aim of developing a genotype independent efficient in vitro regeneration system for Canola (Brassica napus L.) that is not grown in Bangladesh. Plant regeneration was achieved through embryogenic callus production from hypocotyls and cotyledonary leaves with petioles. The optimum medium for callus induction was found with 0.5 mgl-1 2, 4- dichlorophenoxyacetic acid (2, 4-D). The best shooting medium for canola contained 3.0 mgl-1 benzyl amino purine (BAP), 0.1 mgl-1 naphtalene acetic acid (NAA), and 5.0 mgl-1 AgNO3 and produced maximum number of shoots when kinetin was used at a concentration of 0.5 mgl- 1. The profound positive effect of AgNO3 for Canola was found for callogenesis at 0.5 mgl-1 and for regeneration at 5.0 mgl-1. In vitro regenerated shoots of canola produced well developed root system on both the media with 2.5 mgl-1 NAA and 1.0 mgl-1 indole 3-butyric acid (IBA).
Keywords: canola, plant regeneration, and ms medium. GJSFR-C Classification : FOR Code: 970106, 270000
Establishment of Regeneration Protocol for Canola Brassica napus L. Strictly as per the compliance and regulations of :
© 2013. Sabrina Shameen Alam, Laila Khaleda & Mohammad Al-Forkan. This is a research/review paper, distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License http://creativecommons.org/licenses/bync/3.0/), permitting all non commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Establishment of Regeneration Protocol for Canola (Brassica napus L.)
Bangladesh. Plant regeneration was achieved through embryogenic callus production from hypocotyls and cotyledonary leaves with petioles. The optimum medium for callus induction was found with 0.5 mgl-1 2, 4dichlorophenoxyacetic acid (2, 4-D). The best shooting medium for canola contained 3.0 mgl-1 benzyl amino purine (BAP), 0.1 mgl-1 naphtalene acetic acid (NAA), and 5.0 mgl-1 AgNO3 and produced maximum number of shoots when kinetin was used at a concentration of 0.5 mgl- 1. The profound positive effect of AgNO3 for Canola was found for callogenesis at 0.5 mgl-1 and for regeneration at 5.0 mgl-1. In vitro regenerated shoots of canola produced well developed root system on both the media with 2.5 mgl-1 NAA and 1.0 mgl-1 indole 3-butyric acid (IBA).
Keywords: canola, plant regeneration, and ms medium.
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Introduction
anola is the oilseed crop of cold countries, specially, Canada, USA and Europe as cultivation of Canola requires cool temperature during growing season. Bangladesh has a remarkable demand for edible oil. Among the oilseed crops grown in this country, Brassica occupies first position in respect of area and production. In the year of 2003-04, the production was 2.11 lakhs metric ton (Mt), whereas the total oilseed production was 4.0 lakhs Mt (BBS, 2005). Bangladesh consumes around 1.73 million tons of oils per year of which about 1.6 million tons fully met up by import. Hence, it is necessarily important to increase the production of Brassica which can save the country from the huge import pressure. Among the Brassica species, canola is the most productive variety which is low in both erucic acid (
