Evaluation of Etest and Disk Diffusion Methods Compared with Broth ...
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Evaluation of Etest and Disk Diffusion Methods Compared with Broth Microdilution Antifungal Susceptibility Testing of Clinical Isolates of Candida spp. against Posaconazole䌤 Daniel J. Diekema,1,2* Shawn A. Messer,1 Richard J. Hollis,1 Linda B. Boyken,1 Shailesh Tendolkar,1 Jennifer Kroeger,1 and Michael A. Pfaller1,3 Departments of Pathology,1 Epidemiology,2 and Internal Medicine,3 Roy J. and Lucille A. Carver College of Medicine and College of Public Health, University of Iowa, Iowa City, Iowa 52242 Received 10 October 2006/Returned for modification 27 December 2006/Accepted 5 February 2007
We performed Etest, disk diffusion, and broth microdilution susceptibility testing of 2,171 clinical isolates of Candida spp. against posaconazole. By using provisional breakpoints for comparison purposes only, the categorical agreement between the agar-based methods and broth microdilution results ranged from 93 to 98%, with
