bMadras Diabetes Research Foundation, Gopalapuram, Chennai-600086, India. (Received 8 November 1999; In final form 17 June 2000). Altered cytosolic ...
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Evidence for Mechanistic Alterations of Ca 2+ Homeostasis in Type 2 Diabetes Mellitus MUTHUSWAMY BALASUBRAMANYAM a’*, RAMALINGHAM A. BALAKRISHNAN SUBASHINI and VISWANATHAN MOHANb
BALAJIa,
aCenter for Biotechnology, Anna University, Chennai-600025, India;
bMadras Diabetes Research Foundation, Gopalapuram, Chennai-600086, India (Received 8 November 1999; In
final form 17 June 2000)
Altered cytosolic Ca2+ is implicated in the aetiology of many diseases including diabetes but there are few studies on the mechanism(s) of the altered Ca 2+ regulation. Using human lymphocytes, 2+we studied cytosolic calcium (Ca/) and various Ca transport mechanisms in subjects with Type 2 diabetes mellitus and control subjects. Ca2+-specific fluorescent probes (Fura-2 and Fluo-3) were used to monitor the Ca2+ signals. Thapsigargin, a potent and specific inhibitor of the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA), was used to study Ca2+store dependent Ca2+ fluxes. Significant (P
