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Sep 8, 2016 - Quantitation - Comparative C (C) result LAST STRAINS.eds. 2016 Sep 01 10:41:26 PM. 2016 Sep 02 12:53:35 AM. 132 minutes 8 seconds.
Experiment Results Report LAST STRAINS Experiment Summary Experiment Name Experiment Type File Name Run Started Run Finished Run Duration Date Modified User Number of wells used Number of wells with results
: LAST STRAINS : Quantitation - Comparative C (C) : result LAST STRAINS.eds : 2016 Sep 01 10:41:26 PM : 2016 Sep 02 12:53:35 AM : 132 minutes 8 seconds : 2016 Sep 02 12:58:14 AM : : 21 : 21
Instrument Name Instrument Type Comments
: : Applied Biosystems StepOnePlus™ Instrument :
User:
1
Printed:2016 Sep 08 2:48:42 PM
Experiment:LAST STRAINS
Experiment Results Report
Applied Biosystems StepOnePlus™ Instrument
Results Summary Sample
C (Mean)
C (Std Dev)
C (Mean)
C (Std Err)
C
RQ
RQ (Min)
RQ (Max)
Target: CYP4G1 91-C100X
36.9813
11.3382
13.4705
0.0001
91-C10X
30.6732
8.725
10.8573
0.0005
91-R 100X
19.976
-2.5552
-0.423
1.3407
91-R10X
16.2095
-2.9947
-0.8625
1.8181
CS100X
18.1133
-1.6422
0.49
0.712
CS10X *
14.7951
-2.1322
0
1
91-C100X
30.4777
4.8347
-4.0909
17.04
91-C10X
29.9275
7.9792
-0.9463
1.9269
91-R 100X
28.0073
5.4761
-3.4494
10.9236
91-R10X
25.4799
6.2757
-2.6498
6.2759
CS100X
28.796
9.0406
0.1151
0.9233
CS10X *
25.8529
8.9255
0
1
Target: MRP1
Target: RP49 † 91-C100X
25.643
91-C10X
21.9482
91-R 100X
22.5312
91-R10X
19.2042
CS100X
19.7555
CS10X *
16.9274
Notes: (*) denotes Calibrator (†) denotes Endogenous Control
Amplification in negative control Bad passive reference signal Baseline algorithm failed C algorithm failed Exponential algorithm failed High standard deviation in replicate group Multiple Tm peaks No amplification Noise higher than others in plate No signal in well Fluorescence is offscale Outlier in replicate group Noise spikes Thresholding algorithm failed