Experiment Results Report

3 downloads 0 Views 1MB Size Report
Sep 8, 2016 - Quantitation - Comparative C (C) result LAST STRAINS.eds. 2016 Sep 01 10:41:26 PM. 2016 Sep 02 12:53:35 AM. 132 minutes 8 seconds.
Experiment Results Report LAST STRAINS Experiment Summary Experiment Name Experiment Type File Name Run Started Run Finished Run Duration Date Modified User Number of wells used Number of wells with results

: LAST STRAINS : Quantitation - Comparative C (C) : result LAST STRAINS.eds : 2016 Sep 01 10:41:26 PM : 2016 Sep 02 12:53:35 AM : 132 minutes 8 seconds : 2016 Sep 02 12:58:14 AM : : 21 : 21

Instrument Name Instrument Type Comments

: : Applied Biosystems StepOnePlus™ Instrument :

User:

1

Printed:2016 Sep 08 2:48:42 PM

Experiment:LAST STRAINS

Experiment Results Report

Applied Biosystems StepOnePlus™ Instrument

Results Summary Sample

C (Mean)

C (Std Dev)

C (Mean)

C (Std Err)

C

RQ

RQ (Min)

RQ (Max)

Target: CYP4G1 91-C100X

36.9813

11.3382

13.4705

0.0001

91-C10X

30.6732

8.725

10.8573

0.0005

91-R 100X

19.976

-2.5552

-0.423

1.3407

91-R10X

16.2095

-2.9947

-0.8625

1.8181

CS100X

18.1133

-1.6422

0.49

0.712

CS10X *

14.7951

-2.1322

0

1

91-C100X

30.4777

4.8347

-4.0909

17.04

91-C10X

29.9275

7.9792

-0.9463

1.9269

91-R 100X

28.0073

5.4761

-3.4494

10.9236

91-R10X

25.4799

6.2757

-2.6498

6.2759

CS100X

28.796

9.0406

0.1151

0.9233

CS10X *

25.8529

8.9255

0

1

Target: MRP1

Target: RP49 † 91-C100X

25.643

91-C10X

21.9482

91-R 100X

22.5312

91-R10X

19.2042

CS100X

19.7555

CS10X *

16.9274

Notes: (*) denotes Calibrator (†) denotes Endogenous Control

User:

2

Printed:2016 Sep 08 2:48:42 PM

Experiment:LAST STRAINS

Experiment Results Report

Applied Biosystems StepOnePlus™ Instrument

BiologicalGroup Replicate Results

User:

3

Printed:2016 Sep 08 2:48:42 PM

Experiment Results Report

Applied Biosystems StepOnePlus™ Instrument

Plate Layout

Experiment:LAST STRAINS

User:

4

Printed:2016 Sep 08 2:48:42 PM

Experiment:LAST STRAINS

Experiment Results Report

Applied Biosystems StepOnePlus™ Instrument

Gene Expression Plot (RQ vs Target)

User:

5

Printed:2016 Sep 08 2:48:42 PM

Experiment:LAST STRAINS

Experiment Results Report

Applied Biosystems StepOnePlus™ Instrument

Gene Expression Plot (RQ vs Sample)

User:

6

Printed:2016 Sep 08 2:48:42 PM

Experiment:LAST STRAINS

Experiment Results Report

Applied Biosystems StepOnePlus™ Instrument

Gene Expression Plot (RQ vs Target)

User:

7

Printed:2016 Sep 08 2:48:42 PM

Experiment:LAST STRAINS

Experiment Results Report

Applied Biosystems StepOnePlus™ Instrument

Gene Expression Plot (RQ vs Biogroup)

User:

8

Printed:2016 Sep 08 2:48:42 PM

Experiment:LAST STRAINS

Experiment Results Report

Applied Biosystems StepOnePlus™ Instrument

Melt Curve (Derivative Reporter)

User:

9

Printed:2016 Sep 08 2:48:42 PM

Experiment:LAST STRAINS

Experiment Results Report

Applied Biosystems StepOnePlus™ Instrument

Melt Curve (Normalized Reporter)

User:

10

Printed:2016 Sep 08 2:48:42 PM

Experiment:LAST STRAINS

Experiment Results Report

Applied Biosystems StepOnePlus™ Instrument

Results Table Well

User:

Sample

Target

Task

C

C

Tm1

A1

RP49

NTC

29.0665

86.0176

A2

MRP1

NTC

30.7788

79.7547

A3

CYP4G1

NTC

Undetermined

91.9795

A4

CS10X

RP49

UNKNOWN

16.9274

86.6115

A5

CS10X

CYP4G1

UNKNOWN

14.7951

82.1412

A6

CS10X

MRP1

UNKNOWN

25.8529

79.9048

A7

91-C10X

RP49

UNKNOWN

21.9482

86.6106

A8

91-C10X

MRP1

UNKNOWN

29.9275

80.0512

A9

91-C10X

CYP4G1

UNKNOWN

30.6732

82.2914

A10

91-R10X

RP49

UNKNOWN

19.2042

86.7651

A11

91-R10X

MRP1

UNKNOWN

25.4799

80.0524

A12

91-R10X

CYP4G1

UNKNOWN

16.2095

82.4376

B1

CS100X

RP49

UNKNOWN

19.7555

86.6141

B2

CS100X

MRP1

UNKNOWN

28.796

79.9038

B3

CS100X

CYP4G1

UNKNOWN

18.1133

82.7346

B4

91-C100X

RP49

UNKNOWN

25.643

86.9097

B5

91-C100X

MRP1

UNKNOWN

30.4777

79.9048

B6

91-C100X

CYP4G1

UNKNOWN

36.9813

79.7557

B7

91-R 100X

RP49

UNKNOWN

22.5312

87.0579

B8

91-R 100X

MRP1

UNKNOWN

28.0073

80.2003

B9

91-R 100X

CYP4G1

UNKNOWN

19.976

82.4405

11

Tm2

Tm3

61.4118

84.3748

77.5195

Printed:2016 Sep 08 2:48:42 PM

Experiment:LAST STRAINS

Experiment Results Report

Applied Biosystems StepOnePlus™ Instrument

QC Summary Total Wells

96

Processed Wells

21

Targets Used

3

Well Setup

21

Flagged Wells

4

Samples Used

6

Flag AMPNC BADROX BLFAIL CTFAIL EXPFAIL HIGHSD MTP NOAMP NOISE NOSIGNAL OFFSCALE OUTLIERRG SPIKE THOLDFAIL

User:

Name

Frequency

Amplification in negative control Bad passive reference signal Baseline algorithm failed C algorithm failed Exponential algorithm failed High standard deviation in replicate group Multiple Tm peaks No amplification Noise higher than others in plate No signal in well Fluorescence is offscale Outlier in replicate group Noise spikes Thresholding algorithm failed

2 0 0 0 0 0

A1, A2

2 0 0 0 0 0 0 0

A3, A9

12

Locations

Printed:2016 Sep 08 2:48:42 PM