Extracellular DNA contributes to dental biofilm formation
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Extracellular DNA contributes to dental biofilm formation
CLSM image of a 16.5 h old biofilm stained with SYTO 60. (red) and the cell-impermeant DNA stain TOTO-1 (green). Considerable amounts of eDNA can be ...
Extracellular DNA contributes to dental biofilm formation – An ex vivo study
S. Schlafer, R.L. Meyer, I. Dige, V.R. Regina Study design For a long time, research on the biofilm matrix has centered on the role of extracellular polysaccharides. When Whitchurch et al. (Science, 2002) discovered that young P. aeruginosa biofilms could be dispersed by treatment with DNase, extracellular DNA (eDNA) emerged as major structural matrix component.
10 healthy volunteers Custom-made glass slabs (1200 grit) 2.5, 5, 7.5, 16.5 and 24 h biofilms Treatment of adjacent biofilms with DNase or heat-inactivated DNase (1 h; 100 Kunitz) • Quantification of biofilm biovolume in 16200 CLSM images from 300 biofilms
• • • •
Bacteria
The aim of the present study was to investigate whether eDNA is present in young dental biofilms grown in situ in the absence of sucrose, and how treatment with DNase affects biofilm biovolume.
2.5 h
2.5 h
eDNA
HI DNase 7.5 h
DNase 7.5 h
20 µm CLSM image of a 16.5 h old biofilm stained with SYTO 60 (red) and the cell-impermeant DNA stain TOTO-1 (green). Considerable amounts of eDNA can be visualized on bacterial cell surfaces and between microcolonies.
