Paulista (UNESP) â Jaboticabal, SP 3Fundação Educacional de Andradina â Andradina, SP. We thank FAPESP for financial support (Proc. No. 11/21531-9).
UNIV. ESTADUAL PAULISTA “JÚLIO DE MESQUITA FILHO” Faculdade de Medicina Veterinária de Araçatuba
Detection of anti-T. vivax IgG antibodies by IFAT and ELISA in a Trypanosoma vivax outbreak in dairy farm F.A.CADIOLI1, P. H. SAMPAIO2, O.L. FIDELIS JUNIOR2, P. A. BARNABÉ3; C. O. MORALES2, R. Z. MACHADO2, L. C. MARQUES2 1 Faculdade
de Medicina Veterinária – Univ. Estadual Paulista (UNESP) – Araçatuba, SP 2 Faculdade de Ciências Agrárias e Veterinárias – Univ. Estadual Paulista (UNESP) – Jaboticabal, SP 3Fundação Educacional de Andradina – Andradina, SP
The hemoprotozoan Trypanosoma vivax infects a wide range ungulates animals and causes important economic losses in tropical regions of Africa and Central and South Americas. In recent years there has been noted an increase of the number of outbreaks in Brazil in nonendemic areas. The financial impact of this disease can reach 17% of the total herd in endemic areas.
The aim of this work were determine the percentage of cows infected by T. vivax six months after the introduction of this hemoparasite in a dairy farm.
Figure: ELISA levels (EL) and absorbance variation (Abs) of serum samples from cows naturally infected by Trypanosoma vivax during a outbreak in an dairy farm. EL
Abs
Cows (%)
0
0 – 0,193
10(1,97)
1
0,194 – 0,261
2 (0,4)
2
0,262 – 0,328
2 (0,4)
3
0,329 – 0,396
21 (4,14)
4
0,397 – 0,463
30 (5,92)
5
0,464 – 0,531
34 (6,71)
6
0,532 – 0,598
35 (6,90)
7
0,599 – 0,666
49 (9,66)
8
0,667 – 0,733
42 (8,28)
9
≥0,734
282 (55,62)
Total
In this study were used serum samples collected in a dairy herd with 1,080 cows during the first registered outbreak of T. vivax in São Paulo state, Brazil. Whole blood and serum samples of 507 cows, 46.95% of the herd, were collected for molecular and serologic tests; six months after the introduction of infected cows in the herd.
PCR: all samples had 177 bp DNA fragments amplified, which confirms T. vivax diagnosis.
2
Result
Negative
Positive
507 (100)
ELISA is more sensible than IFAT, although IFAT is the more specific serum test for T. vivax diagnosis. The high frequency of anti-T. vivax antibodies indicates fast and efficient transmission of this hemoparasite in dairy farm conditions.
We thank FAPESP for financial support (Proc. No. 11/21531-9).
IFAT: 89.74% (455 animals) positive for anti-T. vivax IgG antibodies and 10, 26% (52 animals) were negatives. ELISA: 93.09% (472 cows) were positive for anti-T. vivax IgG antibodies and 6.91% (35 cows) were negative (Figure).
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