Factors affecting the bond strength of self-etch ...

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Original Article

Factors affecting the bond strength of self-etch adhesives: A meta-analysis of literature P Pranau Vanajasan, Malarvizhi Dhakshinamoorthy1, CV Subba Rao1 Department of Conservative Dentistry, Saveetha Dental College, Saveetha University, 1Department of Conservative Dentistry, Government Dental College, Dr. MGR University, Chennai, India

Abstract Aim: The purpose of this study is to critically evaluate the factors that affect the bond strength of one-step and two-step selfetch adhesives by using meta-analysis. Materials and Methods: Potential papers that were selected according to inclusion and exclusion criteria from articles were published in 13 peer-reviewed journals using “PubMed data base”. From each report, means and standard deviations of bond strengths were extracted and tabulated with corresponding experimental conditions. Results: All the studied parameters showed no significant difference, except for dentin origin/site and bonding area. In addition, statistical analysis done with ANOVA showed statistical significance between the one-step and two-step self-etch adhesives. Conclusions: Our analysis has showed that two-step self-etch adhesive system showed a superior in vitro performance in comparison to one-step self-etch system. Nevertheless, certain factors such as dentin origin, site and area of bonding affect the bond strength of adhesives. Keywords: Bonding agents; meta-analysis review; self-etch adhesive

INTRODUCTION Aesthetic restorative materials have gained considerable importance over a period. Bonding to enamel is been considered satisfactory, but adhesion to dentin is much more complex due to the nature of the substrate. The best way to test the effectiveness of a biomaterial, as well as composite or luting cement or a bonding system, is in vivo experimentation in controlled trials. However, it is quite impossible to distinguish by clinical evaluation and adhesion potential of a dentin-bonding system from many other parameters that influence either the quality or the longevity of a restoration. Theoretically, in vitro bonding tests would allow for measurement of the true strength of bonding of a given system to a dentinal substrate. Unfortunately, in vitro dentin adhesion testing has not been very successful in predicting in vivo effectiveness, probably because of the number of factors influencing test results and clinical relevance.[1-3] Address for correspondence:

Dr. P Pranau Vanajasan, Department of Conservative dentistry and Endodontics, Saveetha Dental Surgery, Saveetha University, Velapanchavadi, Chennai - 600077. Tamil Nadu, India. E-mail: [email protected]

Several authors have already studied the influence of some of these factors, such as the origin of the substrate,[4] substrate storage,[5] dentin depth,[6] type of composite,[7] testing mode,[8] etc. Some previous reviews have listed most of the variables influencing dentin bond strength,[9] but none has quantified their importance or defined their statistical significance. The purpose of this study is to evaluate the true factor that affects the bond strength of one-step and two-step self-etch adhesive. This quantitative overview could be considered as a meta-analysis study performed under nonexperimental conditions to assess the effects of certain clinical parameters on bond strength of adhesives.

MATERIALS AND METHODS Data collection

Potential papers were selected from articles published in 13 peer-reviewed journals using “PubMed data base”. American Journal of Dentistry, Journal of Adhesive Dentistry, Access this article online Quick Response Code:

Date of submission: 24.08.2010 Review completed: 25.08.2010 Date of acceptance: 05.10.2010

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Journal of Conservative Dentistry | Jan-Mar 2011 | Vol 14 | Issue 1

Website: www.jcd.org.in

DOI: 10.4103/0972-0707.80746


Vanajasan, et al.: Self etch adhesives: A meta-analysis

British Dental Journal, Dental Materials, European Journal of Oral Science, International Dental Journal, Journal of Dentistry, Journal of Dental Research, Journal of Oral Rehabilitation, Journal of Prosthetic Dentistry, Operative Dentistry, Quintessence International and Journal of Conservative Dentistry.

We classified the parameters into four groups: Group A Factors related to the dentin substrate; Group B - Type of composite and dentin-bonding agents; Group C - Bonding area and; Group D - Thermocycling and testing mode . All the selected article were summarized in Table 1 according to the commercial products.

Inclusion criteria

Statistical analysis

• Study with original data. • Paper published in English or translated to English. • Only studies measuring the bond strength of one-step and two-step self-etch dentin-bonding agents to be included. • The bonding agents that are commercially available and tested by shear or tensile mode on sound dentin from permanent teeth. • Dentin-bonding agents involved in the analysis should have been tested in at least five different studies. • The outcome measure had to be the dentin bond strength expressed in MPa/Psi. • Light curing unit with intensity of 400-500 mW/cm2 .[10,11] • Among the selected studies, some exclusion criteria applied. • Paper displaying only graphic data. • Dentin-bonding agents not used according to manufacturer’s instructions.[7] • Dentin samples, which were contaminated or not treated under clinical conditions before application of the dentin-bonding agents, were excluded from the analysis • Study without data analysis.

The problem of bond-strength testing is that the materials were seldom compared with a standard. Rather, different materials were tested under various conditions and bond strengths are reported as point estimates and expressed in MPa. It is difficult to compute a standard effect size for each study, as required in a classic meta-analysis. Thus, we decided to analyze directly the measures of bond strength from reports published in literature. This quantitative overview was performed in non-experimental conditions since materials and conditions are not allocated randomly. From each report means and standard deviations of bond strengths were extracted and tabulated with corresponding experimental conditions. The Statistical Analysis Software System MIX 2.0 Professional (Biostat, Englewood, NJ) was used to perform the statistical analysis. Differences between means were computed with analysis of variance.

RESULTS Tables 2 and 3 show descriptive statistics of different parameters tabulated from the selected studies according to the four groups described in materials and methods

Table 1: Selected studies were included in the meta-analysis Commercial products

Manufacturer

Authors

Clearfil S3

Kuraray American Co.

Adper prompt-L-Pop

3M ESPE

G-bond

GC

i-bond

Heraeus Kulzer

One-up bond F plus Optibond all-in-one Xeno III

Tokuyama Dental Corporation Kerr Corporation Dentsply International

Burrow MF et al,[12]; Do Amaral RC et al,[13]; Erickson RL et al [39] Ishikawa A et al,[14]; Owens BM et al,[15]; Perdigão J et al,[16]; Perdigão J et al,[17]; Perdigão J et al,[18]; Reis AF et al,[19]; Reis A et al,[20]; Reis A et al,[21]; Sarr M et al,[22]; Salz U et al,[23]; Soderholm KJ et al,[2]; Van Landuyt KL et al,[22]; Yaseen SM et al [40]; Kimmes NS et al [41] Narasimha Bharadwaj et al,[24]; Burrow MF et al,[12]; Do Amaral RC et al,[13]; Perdigão J et al,[16]; Perdigão J et al,[17]; Perdigão J et al,[18]; R eis AF et al,[19]; Reis A et al,[20]; Van Landuyt KL et al,[32]; Gianini RJ et al,[29]; Kimmes NS et al, [41] Van Landuyt KL et al,[22]; Salz U et al,[23]; Soderholm KJ et al,[2]; Ishikawa A et al,[14] Owens BM et al,[15]; Perdigão J et al,[16] Narasimha Bharadwaj et al,[24]; Van Landuyt KL et al,[22]; Salz U et al,[23]; Reis A et al,[20]; Perdigão J et al,[18]; Soderholm KJ et al,[2]; Perdigão J et al,[17]; Reis AF et al,[19]; Owens BM et al,[15]; Perdigão J et al,[16] Burrow MF et al,[12]; Van Landuyt KL et al,[22]; Britta LC et al, [44] Salz U et al,[23]; Van Landuyt KL et al,[22]; Kimmes NS et al [41] Narasimha Bharadwaj et al,[24]; Do Amaral RC et al,[13]; Landuyt KL et al,[22]; Kimmes NS et al, [41]

One-step self-etch adhesives

Two-step self-etch adhesives (SEP) Clearfil SE

Kuraray American Co.

AdheSE

Ivoclar Vivadent

Optibond solo plus

Kerr Corporation

Narasimha Bharadwaj et al,[24]; Burrow MF et al,[12]; Reis A et al,[20]; Kimmes NS et al (2010) Sarr M et al,[22]; Lee JI et al, [43] Gianini RJ et al, [42]; Kimmes NS et al, [41] ; Britta LC et al, [44] Kimmes NS et al,[41]


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Vanajasan, et al.: Self etch adhesives: A meta-analysis Table 2: Descriptive statistics of the different parameters studied using one-step self-etch adhesives I

Parameters

Description

Origin of dentin

Human teeth Non-human teeth Incisor Molar Occlusal Non-occlusal One-step self-etch Packable Flowable Enamel Enamel and dentin Yes No Shear Tensile Micro-tensile

Type of teeth Site of test II

Dentin bonding agents Type of composite

III

Bonding area/dentin depth

IV

Thermocycling Testing mode

N

Mean

SD

P value

470 30 7 25 129 81 83 80 20 32 100 5 132 20 50 10

18.2 14.7 19.5 16.1 17.9 15.7 17.2 15.7 13.9 17.9 12.9 14.6 13.3 14.5 17.8 19.5

6.8 5.2 7.8 5.1 8.6 5.9 8.4 4.7 5.3 8.9 3.8 7.6 5.8 6.2 4.6 4.1

0.03 0.025 0.124 0.023 0.09 0.02

Table 3: Descriptive statistics of the different parameters studied using two-step self-etch adhesives I

Parameters

Description

Origin of dentin

Human teeth Non-human teeth Incisor Molar Occlusal Non-occlusal Two-step self-etch Packable Flowable Enamel Enamel and dentin Yes No Shear Tensile Micro-tensile

Type of teeth Site of test II

Dentin-bonding agents Type of composite

III

Bonding area

IV

Thermocycling Testing mode

section. Some parameters, such as tooth storage medium and dentin surface topography, were not included in the statistical analysis, due to their greater variability or lack of information. It was noted that about one-third of the selected articles provided such information and that the distinction between the different failure modes was not always available and clearly identified. For this reason, both composite and dentin cohesive failures were grouped together. One-way ANOVA comparison between one-step and two-step self-etch adhesives is given in Table 4 The P-values indicate the potential relation between each parameter and the bond strength. Their respective influences were given by the P-value at the significance level of 0.05(α). All the studied parameters showed no significant difference, except for dentin origin/site and bonding area. In addition, statistical analysis done with ANOVA showed statistical significance between the one-step and two-step self-etch adhesives.

DISCUSSION As quoted by DerSimonian and Laird (1986) “Meta-analysis

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N

Mean

SD

P value

187 2 75 63 120 4 200 154 41 50 150 5 192 30 75 14

17.7 11.8 18.2 16.5 19.5 18.2 19.9 15.6 13.2 20.9 18.7 14.6 13.4 18.2 15.2 20.1

8.6 3.4 4.9 5.5 4.9 4.9 4.7 2.5 6.7 4.9 2.4 4.5 6.8 4 2.4 4.9

0.1 0.02 0.103 0.02 0.1 0.03

is the statistical analysis of a collection of analytic results for the purpose of integrating the findings.” The advantage of meta-analysis over the traditional literature review is the possibility of combining results from many studies and analyzing the factors that affect the bond strength of selfetch adhesives.[25] In the studies selected, the tensile or shear testing was done using a constant crosshead speed and the samples were continuously loaded such that the crack will propagate at increasing speed until separation. In a clinical situation, the propagation kinetics of the crack is completely different. During function, an assembly is submitted to a series of brief loads. If these loads are high enough, small extensions of cracks ensure sub-critical crack growth in bonded area as explained by A. A. Griffith. According to Griffith’s theory of brittle solid it is stated that the low fracture strength observed in experiments, as well as the size-dependence of strength, were due to the presence of microscopic flaws in the bulk material.[26] In this practical situation, the crack speed is much lower than during tensile or shear forces, which is used to determine the adhesion potential of a



Vanajasan, et al.: Self etch adhesives: A meta-analysis Table 4: Comparison between one-step and two-step self-etch adhesives using ANOVA Groups

Parameters

I

Origin of dentin

II III IV

Description

Human teeth Non-human teeth Type of teeth Incisor Molar Site of test Occlusal Non-occlusal Type of composite Packable Flowable Bonding area/dentin depth Enamel Enamel and dentin Thermocycling Yes No Testing mode Shear Tensile Micro-tensile

P value 0.07 0.11 0.27 0.23 0.1 0.01 0.04 0.01 0.02 0.11 0.04 0.003 0.12 0.1 0.08

dentin-bonding system. Conventional tests may be simple and fast but they have many drawbacks. The location of the initial flaw is unknown, and the stress concentration at the bonded interface is non-uniform and unpredictable. Moreover, the stress distribution is highly dependent on the test geometry, on the loading mode and on the material properties.[27-29] Our analysis revealed that the origin of dentin, site of bonding and bonding area had significant influence on bond strength of one-step and two-step self-etch adhesive. The studies have also showed that there is a difference in bond strength between human teeth and bovine teeth. This difference explains the fact that dentinal tubules are large in the coronal portion of bovine dentin, but one study by Nakamichi et al,[30] showed that there is no difference in bond strength between bovine and human dentin when only the superficial layers was used. Studies also showed that the bond strength varied between buccal and occlusal surfaces. Oilo and Olsson[31] showed that the tensile strength is higher on the buccal surface compared to the occlusal surface, which could be explained by the fact that the tubules are lower in number with less area percentage on the buccal surface than on the occlusal surface. The analysis also showed a definite difference between enamel and dentin bonding which might be explained by various reasons. Dentin has a heterogeneous structure and different orientation of tubules with root dentin having smaller and more numerous branching of tubules when compared to crown dentin. Acidity of monomer also caused change in surface chemistry and morphology of dentin, which in turn can influence bonding. A significantly thicker hybrid layer was noted in areas with perpendicular tubule orientation than in areas with parallel tubule orientation[32]

Surface area significantly affects the bond strength in onestep and two-step self-etch adhesives. Bond strength is calculated as the load at failure divided by the cross-sectional area of the bonded surface. An inverse relationship between bond strength and bonded surface area has been recently shown,[33] confirming previous studies.[34] These authors explain the lower strengths of large bonded samples by the greater number of defects within the adhesive joint. Burrow MF et al, stated that Clearfil SE Bond showed no statistical difference to G-Bond.[12] Do Amaral RC et al, suggested application of bonding agents with agitation on the dentin surface to improving the resin-dentin bond strength of one-step self-etch adhesives.[13] When bonded to dentin, the adhesives with simplified application procedures (in particular, one-step self-etch adhesives) still underperform as compared to conventional threestep adhesives. “Mild” two-step self-etch adhesives that provide additional chemical bonding, appear to most optimally combine bonding effectiveness with a simplified application protocol[22] Our analysis has showed that two-step self-etch adhesive system showed a superior in vitro performance in comparison to one-step self-etch system. These results are in accordance with those of other studies.[14, 24, 35, 36, 38] The bonding ability of the all-in-one adhesives depends on their specific composition. In light of the low in vitro bond strengths and high rate of spontaneous failures of some all-in-one adhesives compared to those of the two-step adhesives, the newest adhesives should be screened more strictly before they are recommended for clinical use.[14] One-step self-etch adhesives showed higher bond strengths on ground enamel and no reductions in resin-enamel bonds were observed after 12 months of water storage. Marginal adaptation was significantly better for Clearfil S3 Bond than for Adper Prompt L-Pop.[15] Serious limitation of all-in-one adhesives are as follows: incomplete polymerization and continued demineralization of the adjacent dentin structure in the tubules. For all-inone adhesives to be acidic, the formulation have become more hydrophilic, thereby allowing deeper penetration. As these adhesives penetrate the wet dentinal tubules deeply, the water content increases. Studies have shown that this water acts as a major interfering factor in polymerization which leads to unpolymerized acidic and aggressive monomers to continue etching the dentin, thereby leading to a detrimental impact on the bond.[35] Other factor leading to low bond strength and failure of resin-based adhesives in vivo are harsh conditions of the oral environment, such as intraoral temperature, moisture contact, fatigue of bond due to tooth flexure and bacterial enzymes.[37]


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Vanajasan, et al.: Self etch adhesives: A meta-analysis

The shelf life of one-component self-etching adhesives is determined by their chemical composition. In conventional methacrylate-based adhesives (AdheSE), the inherently acidic environment of such formulations leads to monomer degradation due to hydrolysis. In contrast, methacrylamidebased adhesives are stable to aqueous acid and exhibit much superior storage stability without monomer degradationrelated losses in adhesion performance.[23] Intermediary strong' self-etch adhesives (AdheSE and Xeno III) exhibited significantly superior shear bond strength values compared to both `strong' (Prompt-L-Pop) and `weak' (Clearfil SE Bond) self-etch systems, which implies that pH of the adhesive has a significant effect on the bond strength.[24] Another major variable in bond strength tests is the depth of dentin. Suzuki and Finger[1] found a linear relationship between the remaining dentin thickness to the pulp and the shear bond strengths of three different adhesives, an observation subsequently confirmed by other authors[6,16] The analysis surprisingly showed that type of composite and thermocycling did not significantly affect bond strength. Nevertheless, three studies reported a decrease in adherence after thermocycling[31,32] whereas other studies reported an increase, depending on the adhesive system tested.[31]

9. 10. 11. 12. 13.

14. 15. 16. 17. 18. 19. 20. 21.

22.

CONCLUSION From this meta-analysis study, we have analyzed that twostep self-etch adhesive showed better bonding ability than one-step self-etch adhesives. Thereby, several clinical parameters among which dentin origin, site and area of bonding have shown a significant influence on mean bond strength of adhesive system. Thus, our study suggests, that the practitioners can easily control these parameters by using standardized protocol for the clinical success of selfetch adhesive systems.

23. 24.

25. 26. 27. 28.

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Source of Support: Nil, Conflict of Interest: None declared.

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