Feasibility of Transferring Fluorescent In Situ Hybridization Probes to ...
Recommend Documents
Microautoradiographyâa New Tool for. Structure-Function Analyses ..... signature region of the 16S rRNA of most halophilic and halotolerant ammonia oxidizers ...
Here we described a robust and reliable method for mapping genome sequences to C. intes- tinalis chromosomes using FISH to metaphase spreads pre-. F. A.
Mar 29, 2016 - Responsible Editor: Hans de Jong. S. Hesse .J. Fuchs . ..... Kelly RB, Cozzarelli NR, Deutscher MP, Lehman IR, Kornberg A. (1970a) Enzymatic ...
Vidal, F., J. Mensa, M. Almela, J. A. Martinez, F. Marco, C. Casals, J. M.. Gatell, E. Soriano, and ... EPIC International Advisory Committee. JAMA 274:639â644.
Jul 21, 2006 - Mailing address: Dako UK Ltd., Den- mark House, Angel Drove, ... E-mail: jeremy [email protected]. .... for automation. Thus, a much-higher ...
Conventional cytogenetic analysis and fluorescence in situ hybridization (FISH) results of bone marrow samples of 36 multiple myeloma (MM) patients at the ...
Infectious Diseases Research Center, Bushehr University of Medical Sciences, Bushehr (Iran). 2Department of Microbiology, Islamic Azad University, Jahrom ...
Oct 19, 2017 - High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and .... 160 µL of cold 70 % EtOH (RNase-free) for 30 min at 2,250 x g at 4 °C. .... mass (boxes) fly rearing, use standard fly lab protocols on cornmeal ...
Feb 2, 2012 - of the particle17. Images of FISH-hybridized yeast cells are acquired .... analyze a large number of mRNAs simultaneously and rapidly rep- resents a ... ever, the number of uniquely labeled mRNAs can increase when a specific color ....
Abstract. â Background and Objectives: Streptococcus pyogenes (S. pyogenes) is an impor- tant cause of pharyngitis. Rapid detection of this microorganism in ...
Dec 10, 2001 - Susan Rigby,1 Gary W. Procop,2 Gerhard Haase,3 Deborah Wilson,2 Geraldine Hall,2. Cletus Kurtzman,4 Kenneth Oliveira,1 Sabina Von Oy,3 ...
polymerases for directly detected FISH (e.g., from Molecular Probes Inc., Eugene, ...... Landegent, J. E., Jasen in De Wal, N., Baan, R. A., Hoeijmakers, J. H., and ...
1California Institute of Technology, Pasadena, CA and 2The University of ... 1000 Sectioning System from Technical. Products ..... by the NSF CAREER grant no.
Jun 1, 2005 - merism quantification by FISH on routine smears: easier, faster and more sensitive than STR-PCR. Blood 100:5271. 5. da Silva, C. C., and A. D. ...
VETERINARSKI ARHIV 82 (5), 495-504, 2012. Fluorescent in-situ ... Key words:
classical swine fever virus, fluorescent in-situ hybridization, virus localization ... a
genome of approximately 12.3 kb (MOORMANN et al., 1990; HA et al., 2004).
delineation of an X:Y translocation in a patient with secondary ... material involving the long arm of chromosome X (Xq28) and the long arm of chromosome Y ...
1Universidade Estadual do Sudoeste da Bahia, Departamento de Ciências Naturais, Vitória da Conquista,. BA, Brazil. 2Universidade Federal de Viçosa, ...
Boom, R.; Geelen, J. L.; Sol, C. J.; Raap, A. K.; ... Raap, A. K.; Van de Rijke, F .M.; Dirks, R. W.; Sol, C. J.; ...... Schöfer, C.; Müller, M.; Leitner, M. D.; Wachtler, F.
F. Illustration of a chromosomal translocation ..... Figure 1: Schematic illustration of âcomparative ...... and basic methodology of DNA/RNA detection by in situ ...
In situ hybridization techniques allow specific nucleic acid sequences to be detected in morphologically preserved chromosomes, cells or tissue sections. In.
applicability of in situ hybridization tech- niques, their use ... DN A recombinant technology. ...... guide to help solve hybridization problems ...... the mixture. â· Cool the mixture to â20°C in the freezer. â¢2 Perform infiltration at about
Feasibility of Transferring Fluorescent In Situ Hybridization Probes to ...
Sep 17, 2007 - Lange, M., L. Guillou, D. Vaulot, N. Simon, R. I. Amann, W. Ludwig, and ... Lindblad-Toh, K., E. Winchester, M. J. Daly, D. G. Wang, J. N. ...
Feasibility of Transferring Fluorescent In Situ Hybridization Probes to an 18S rRNA Gene Phylochip and Mapping of Signal Intensities䌤 Katja Metfies and Linda K. Medlin* Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, D-27570 Bremerhaven, Germany Received 17 September 2007/Accepted 28 February 2008
DNA microarray technology offers the possibility to analyze microbial communities without cultivation, thus benefiting biodiversity studies. We developed a DNA phylochip to assess phytoplankton diversity and transferred 18S rRNA probes from dot blot or fluorescent in situ hybridization (FISH) analyses to a microarray format. Similar studies with 16S rRNA probes have been done determined that in order to achieve a signal on the microarray, the 16S rRNA molecule had to be fragmented, or PCR amplicons had to be
