Fig S4_2.pptx - Plos

trans-splicing site （-193）

Premature mRNA

A

100 bases +1

-761

Reverse transcriptase AUG

Ser/Thr kinase UAG AUG

Putative Retrotransposon Ser/Thr kinase

SL sequence

B -220

SL trans-spliced mRNA

trans-splicing site (-193)

-210

-200

-190

-180

-170

-40

-30

-20

-10

+1

+10

// ・・・・・・aaaggaauaauaaaaccuauuuauaaag GUAGAGGAGAUAGGCUAAAUGCCUCU・・ ・・・CAGCGGUACGAGAAAAGGUAAACGAGUAGCUUUGAAGAUGUUC AUGGUGGAUGCUAA・・・・・・

// ・・・CAGCGGUACGAGAAAAGGUAAACGAGUAGCUUUGAAGAUGUUC AUGGUGGAUGCUAA・・・・・・ CUGGAUAAUCCGGCUUUUCUG GUAGAGGAGAUAGGCUAAAUGCCUCU・・

SL sequence

Ser/Thr kinase

S4 Fig. A case of SL trans-spliced mRNA rescued from the disturbance by the retrotransposon inser>on just upstream of the coding region. A. Schema'c models of the premature and SL trans-sliced forms of the transcripts encoding a serine/threonine kinase. These RNA sequences were searched from the RNA-seq data of P. micropora obtained by Illumina HiSeq 4000. B. Nucleo'de sequences covering the trans-splicing site and transla'on ini'a'on context of the transcripts modeled in A, with the 3’ splicing acceptor mo'f (AG), termina'on codon, and ini'a'on codon shown by red characters.