Figure 4A,B,C chemograms. JQ1 Concentration (mM Mean. SD. N. Mean. SD. N. Mean. SD. N. Mean. SD. N. Mean. SD. N. Mean. SD. N. Mean. SD. N. Mean. SD.
Figure 4A,B,C chemograms JQ1 Concentration (mM 0,000 0 0,001 0,001 0,003 0,003 0,010 0,01 0,030 0,03 0,100 0,1 0,300 0,3 1,000 1 3,000 3 10,000 10 30,000 30
figure 4D
Mean 100 90 86 82 82 79 74 60 51 44 37
(CRCM17) SD 10,92 4,48 3,3 3,85 2,16 2,57 2,55 0,53 0,38 1,6 0,86
N
Mean 3 3 3 3 3 3 3 3 3 3 3
100 101 102 102 101 98 76 51 29 21 14
(CRCM16) SD 7,64 7,02 3,05 2,26 3,64 7,94 0,58 3,67 1,08 0,21 0,09
N
Mean 3 3 3 3 3 3 3 3 3 3 3
100 100 100 98 96 91 78 55 38 24 12
spheroids assay
MYC low DMSO 86,83876 100,2606 84,67612 88,29445 86,40707 124,1886 109,7308 88,92326 86,83876 81,12198 75,30049 86,00406 91,30586 110,6883 107,812 90,41254 86,83876 95,02122 114,7981 124,5452 139,7758 126,3671 137,7167 76,13335
figure 4E
JQ1 (2µM) 98,67584 66,09727 75,6963 75,83363 77,73877 135,6698 86,33214 69,94681 256 67,34264 56,34584 59,71405 52,46368 45,46173 71,49242 68,27697 69,70473 60,61635 81,55502 81,39976 120,611 86,22461 76,62412 89,24203
MYC high DMSO 88,33624 83,43211 102,945 106,2216 126,8207 105,9222 119,2664 103,4209 88,33624 129,6242 132,5938 106,4214 121,864 113,1813 95,83772 91,92612 88,33624 92,11407 68,18607 70,98103 84,74858 83,49 101,0047 94,98939
JQ1 (2µM) 58,7326 51,31942 49,91767 53,19592 56,25889 56,52087 47,15796 64,49735 33,74336 32,83791 33,80598 40,19901 45,27991 43,87851 42,53554 41,73729 39,95705 37,36454 45,92672 50,39285 49,97701 36,30051 46,12014 33,89827
densitometry Western blot
normalisation DMSO = 1 DMSO 100 nM 250 nM 500 nM 1000 nM 2000 nM DMSO 100 nM 250 nM 500 nM 1000 nM 2000 nM DMSO 100 nM 250 nM 500 nM 1000 nM 2000 nM
MYC/Actin 0,73648784 0,49307371 0,50308586 0,33728148 0,29555753 0,21431798 p27/Actin 0,34180971 0,3779941 0,59921737 0,5622749 0,69527442 0,6573687 CAS3/Actin 0,04957735 0,11034718 0,12338484 0,3824577 1,09666435 1,34567988
0,73028444 0,47795068 0,49665425 0,31892923 0,28686861 0,20733268
0,78308616 0,52436556 0,54147803 0,36787666 0,33496337 0,24543298
1 0,66949334 0,6830878 0,45795933 0,40130674 0,29100002
1 1 0,65447194 0,66961414 0,68008329 0,69146673 0,43671918 0,46977802 0,39281764 0,42774778 0,28390675 0,3134176
0,33964859 0,37749772 0,59375872 0,55724952 0,69119709 0,65093491
0,3485488 0,38076626 0,59629197 0,55610137 0,69431461 0,67611388
1 1,10586123 1,75307301 1,64499396 2,03409796 1,92320082
1 1,11143615 1,74815597 1,64066489 2,03503591 1,91649524
1 1,09243311 1,71078473 1,59547635 1,99201548 1,93979686
0,04590744 0,10614516 0,1180598 0,37766744 1,0935521 1,32856527
0,06102248 0,12488895 0,14301975 0,41355253 1,14402136 1,41241617
1 2,22575792 2,48873404 7,71436315 22,1202687 27,1430368
1 2,31215588 2,57169199 8,22671536 23,8208032 28,9400861
1 2,04660559 2,3437222 6,77705183 18,7475387 23,1458325
(CRCM04) SD 7,89 4,18 6,18 7,49 9,41 3,83 4,38 0,43 0,79 0,34 0,13
N
Mean 3 3 3 3 3 3 3 3 3 3 3
100 89 84 84 86 77 71 52 42 32 23
(CRCM110) SD 1,35 4,9 2,3 4,77 0,41 1,4 1,25 1,02 1,14 0,69 0,47
N
Mean 3 3 3 3 3 3 3 3 3 3 3
100 98 91 93 93 89 83 70 61 55 50
(CRCM05) SD 5,71 5,86 1,89 1,06 3,09 0,96 3,13 1,09 1,41 0,11 0,91
N
Mean 3 3 3 3 3 3 3 3 3 3 3
100 99 101 99 101 100 92 86 75 70 65
(CRCM10) SD 9,76 1,02 4,71 1,52 1,71 4,68 3,53 0,59 0,42 2,28 1,38
N
Mean 3 3 3 3 3 3 3 3 3 3 3
100 101 95 95 94 91 83 69 59 59 55
(CRCM11) SD 9,32 7,02 2,9 5,06 3,74 3,57 0,89 2,1 2,1 1,39 0,67
N
Mean 3 3 3 3 3 3 3 3 3 3 3
100 97 105 100 104 97 89 83 68 64 48
(CRCM109) SD 9,08 6,86 3,76 1,46 2,41 3,51 1,38 5,76 1,32 3,03 0,65
N 3 3 3 3 3 3 3 3 3 3 3
Figure 4. E : source data for western blots
β actin
C-MYC
Non specific band Non specific band C-MYC
p27KIP1
Non specific band
Cleaved Casp3
Non specific band
