Figure S1. Wild type and bisulfite converted

Figure S1. Wild type and bisulfite converted sequences of studied regions with positioned PCR and SIRPH primers. P16 (cyclin-dependent kinase inhibitor 2A) on NT_008413 (Genbank accession number) Wild type sequence 21954841 ccactgcact 21954901 aaaaaagttg 21954961 ggaaggaggt 21955021 gcactggCGt 21955081 ggaagggctg 21955141 ctCGtcaccC 21955201 agggcctttt 21955261 ttctttCGtc 21955321 tgcagcctca 21955381 acccttcccc 21955441 gcttCGgagg

ccagcctggg ttttctgcta ggaggtgaag gggccccagg ctctccCGtc GCGctCGtga attgcccttc CGtggttttg gggcctCGaa tctggcttct ttactgCGgc

CGacagagaa tttcctgaac accctcccac aaggatccca cCGCGggctc CGCGtgctta tgctccacag gaaagtcccC cagccatgct tttttttcac ctcctccaCG

agactcCGtc tttattaCGt ctggcctgca gccagttttg CGaggtCGcC caaaggaaac ccccCGtgct Gcctccaaac tagaatcCGt tggagaaagc ggtagttctg

Bisulfite converted sequence 21954841 ttattgtatt ttagtttggg CGatagagaa agatttCGtt 21954901 aaaaaagttg ttttttgtta ttttttgaat tttattaCGt 21954961 ggaaggaggt ggaggtgaag atttttttat ttggtttgta Bi-p16-F1 21955021 gtattggCGt gggttttagg aaggatttta gttagttttg 21955081 ggaagggttg ttttttCGtt tCGCGggttt CGaggtCGtC 21955141 ttCGttattC GCGttCGtga CGCGtgttta 21955201 21955261 21955321 21955381 21955441

agggtttttt ttttttCGtt tgtagtttta attttttttt gtttCGgagg

attgtttttt CGtggttttg gggtttCGaa tttggttttt ttattgCGgt

tgttttatag gaaagttttC tagttatgtt ttttttttat tttttttaCG

tcaaaaaaaa aaatgagaca tccagagtac gtgctggagg GcaccCGggC ttttacaggt taatCGgttg CGcagtccCG ccttctttcc ccaccCGagt ggaccCGggt

ttaaaaaaaa aaaaaaaaaa aaatgagata CGtgagattt tttagagtat ttggggtgtg

gtgttggagg ttCGgttaaa GtattCGggC GCGtttgggt SN-2 taaaggaaat ttttataggt ttCGgtgggt Bi-p16-R1 ttttCGtgtt taatCGgttg gttgggaggt Gtttttaaat CGtagtttCG tagttagttt tagaattCGt tttttttttt tgttttattt tggagaaagt ttattCGagt tttttagttt ggtagttttg ggattCGggt ttagttCGCG

SIRPH Primer P16 SN-2 (-2585 bp from the transcription start site)  

aaaaaaaaaa CGtgagatct ttggggtgtg ccCGgccaaa GCGcctgggc ttCGgtgggc gttgggaggt cagtcagttc tgctccatct ctctcagcct ctagtcCGCG

APC (adenomatosis polyposis coli) on NT_034772 (Genbank accession number) Wild type sequence 14488201 ttctctgtgc 14488261 cCGgcatctt 14488321 tcaactgcca 14488381 gggtaCGggg 14488441 gagtgCGggt 14488501 gCGctcccca 14488561 atgtggctgt 14488621 GgactCGgaa 14488681 gggaaggtgg 14488741 actgcagcat 14488801 ggagatggat

tgcaaaaatc gtgctaatcc tcaacttcct ctagggctag CGggaagCGg ttccCGtCGg attggtgcag atggggtagg ttttccctCG gccaaaCGag ttcctgtact

atagcaatCG ttctgccctg tgcttgctgg gcaggctgtg agagagaagc gagccCGcCG ccCGccaggg tgctggagcc cactgtctta gaggcagggg tCGgatccag

agatgtaatt CGgacctccc ggactggggc CGgttgggCG agctgtgtaa attggctggg tgtcactgga accatggcca aacCGatggc CGtCGtcccc ggtttttgac

tattactctc cCGactcttt CGCGagggca gggccctgtg tcCGctggat tgtgggCGca gacagaatgg ggcttgctgC ctttccttgg cCGcccccca agaagaggaa

Bisulfite converted sequence 14488201 ttttttgtgt tgtaaaaatt atagtaatCG agatgtaatt tattattttt 14488261 tCGgtatttt gtgttaattt ttttgttttg CGgatttttt tCGatttttt 14488321 ttaattgtta ttaatttttt tgtttgttgg ggattggggt CGCGagggta Bi-APC-F1 14488381 gggtaCGggg ttagggttag gtaggttgtg CGgttgggCG gggttttgtg SN-1 14488441 gagtgCGggt CGggaagCGg agagagaagt agttgtgtaa ttCGttggat SN-2 14488501 gCGtttttta ttttCGtCGg gagttCGtCG attggttggg tgtgggCGta Bi-APC-R1 14488561 atgtggttgt attggtgtag ttCGttaggg tgttattgga gatagaatgg 14488621 GgattCGgaa atggggtagg tgttggagtt attatggtta ggtttgttgC 14488681 gggaaggtgg ttttttttCG tattgtttta aatCGatggt ttttttttgg 14488741 attgtagtat gttaaaCGag gaggtagggg CGtCGttttt tCGtttttta 14488801 ggagatggat tttttgtatt tCGgatttag ggtttttgat agaagaggaa

cctcccacct actatgCGtg taccccCGag ccccactgCG gCGgaccagg CGtgacCGac aggtgctgcC Ggggggaggg cacagggtcc ctgcagcact gaagggggag

ttttttattt attatgCGtg tattttCGag ttttattgCG gCGgattagg CGtgatCGat aggtgttgtC Ggggggaggg tatagggttt ttgtagtatt gaagggggag

SIRPH Primer SN-1 (-131 bp from the transcription start site) and SN-2 (-75 bp from the transcription start site)

3OST2 (HS3ST2, Hepara sulfat-D-glucosaminyl-3-0-sulfotransferase-2) on NT_010393 (Genbank accession number) Wild type sequence 14138701 GCGctcCGaa 14138761 gccccatCGc 14138821 ctgccccCGg 14138881 CGcccccaaC 14138941 cagggccaca 14139001 ccatggagcc 14139061 GCGcaggctg 14139121 cctgtgctgc 14139181 CGgccccagC 14139241 gcCGaCGccc 14139301 cctctcCGgt 14139361 cctcattgtg 14139421 ccCGgaCGtg 14139481 gctggattgg

ccCGgCGcaC ctaggacCGg gaccccctgg GccCGaccCG gcagctcagc atggcctata ctcttCGcct tgCGaCGacc GCGggCGgcc agCGagccca tccaaccact ggCGtgaaga CGggccttgg tacaggtaag

Gtaagagcct gagatgctgg cactgtgCGc CGtggcCGtg CGcCGgtgcc gggtcctggg tcaCGctctC tgggtCGgag agaaacttct gCGctcccag cCGgctcacc aggggggcac gcaCGgaacc gaccaggagc

gggagCGccC aaatgcaacC accctggtca gcagCGccaC ccctCGgaaa cCGCGCGggg Gctctcctgc cCGcctcctC ccagaagtcc CGCGccCGcC caagctgggt cCGggcCGtg ccacttcttt tcCGctcCGt

ttCGgCGtaC ttaggatCGg gattttttgg GttCGattCG gtagtttagt Bi-3OST2-F2 14139001 ttatggagtt atggtttata SN-2 14139061 GCGtaggttg tttttCGttt

Gtaagagttt gagatgttgg tattgtgCGt CGtggtCGtg CGtCGgtgtt

gggagCGttC aaatgtaatC attttggtta gtagCGttaC ttttCGgaaa

Bisulfite converted sequence 14138701 GCGtttCGaa 14138761 gttttatCGt 14138821 ttgttttCGg 14138881 CGtttttaaC 14138941 tagggttata

GagcCGccCG Gcctgttccc gcagccccCG GCGagccctc ccatgacccc ccacctcagc acttacctgt GgCGCGcctC CGcccctgtg GcCGcCGtgc accaagCGgt ctggagttta gacaggaact gCGcCGggtc

gctgccCGga CGaggagcCG gagaagaCGg taggCGacCG CGgCGCGggc CGCGgagggC gttacagctt GctgcctcCG atccctcCGg cCGcccctCG tgccccaagc tcCGagtaca aCGgcCGCGg tctgatCGct

GagtCGttCG Gtttgttttt gtagttttCG GCGagttttt ttatgatttt SN-1 gggttttggg tCGCGCGggg ttattttagt

gttgttCGga CGaggagtCG gagaagaCGg taggCGatCG CGgCGCGggt CGCGgagggC

ttaCGttttC Gtttttttgt atttatttgt gttatagttt

14139121 tttgtgttgt tgCGaCGatt tgggtCGgag tCGttttttC Bi-3OST2-R2 14139181 CGgttttagC GCGggCGgtt agaaattttt ttagaagttt 14139241 gtCGaCGttt agCGagttta gCGtttttag CGCGttCGtC 14139301 ttttttCGgt tttaattatt tCGgtttatt taagttgggt 14139361 ttttattgtg ggCGtgaaga aggggggtat tCGggtCGtg 14139421 ttCGgaCGtg CGggttttgg gtaCGgaatt ttattttttt 14139481 gttggattgg tataggtaag gattaggagt ttCGtttCGt 14139541 tttattggga gagttattCG ttttttgtgt tttttttttt

GgCGCGtttC GttgttttCG CGtttttgtg GtCGtCGtgt attaagCGgt ttggagttta gataggaatt gCGtCGggtt ttttaattta

attttttCGg tCGtttttCG tgttttaagt ttCGagtata aCGgtCGCGg tttgatCGtt atttattgta

SIRPH Primer SN-1 (+99 bp from the transcription start site) and SN-2 (+124 bp from the transcription start site)

DAP-KINASE (DEATH ASSOSIATED PROTEIN KINASE 1) on NT_023935 (Genbank accession number) Wild type sequence 19278181 tggggaCGcC 19278241 gtCGggggCG 19278301 aggggcagct 19278361 ttccttttgg 19278421 ggagactgat 19278481 Ggagctgaag 19278541 aggaaaaCGt 19278601 agaagCGtac 19278661 aatgcagttt 19278721 aagagttact

GacctCGggg tccctggcCG cCGgagaccC ggttgccatt gcatgagggg tgccctgggc ggatgattac cctcctggat gaatcaggCG aacccagCGg

tgcCGtggtg cccagcttta GgtcttcagC ttactattat gctaCGgagg tttggtgagg taCGacacCG tgtggaaatg tctccctCGc taaacCGcct

ccCGgcccca acaaagggtg GagCGgggtc tattgccttt CGcaggagCG CGtgacagtt gCGaggaact cataaCGatg cctttctgga gatccaaggg

CGCGCGCGCG ctcctctcca ttagCGcCGg tttttttctt gtggtgatgg tatcatgacC tggcaggtaa gggccattgg gatgCGcaaa cctgggggtg

gggctgaggg cccCGCGagg ggaggtctac caaaaggact tctgggaagC Gtgttcaggc agggggtacc gtggtaaaca tcatagagaa gaggagaggc

Bisulfite converted sequence 19278181 tggggaCGtC GatttCGggg tgtCGtggtg ttCGgtttta CGCGCGCGCG gggttgaggg 19278241 gtCGggggCG tttttggtCG tttagtttta ataaagggtg ttttttttta tttCGCGagg 19278301 aggggtagtt tCGgagattC GgtttttagC GagCGgggtt ttagCGtCGg ggaggtttat Bi-DAPK-1-F1 19278361 ttttttttgg ggttgttatt ttattattat tattgttttt tttttttttt taaaaggatt 19278421 ggagattgat gtatgagggg gttaCGgagg CGtaggagCG gtggtgatgg tttgggaagC 19278481 Ggagttgaag tgttttgggt SN-3 19278541 aggaaaaCGt ggatgattat 19278601 agaagCGtat ttttttggat 19278661 aatgtagttt gaattaggCG 19278721 aagagttatt aatttagCGg

tttggtgagg CGtgatagtt tattatgatC Gtgtttaggt Bi-DAPK-1-R1 taCGatatCG gCGaggaatt tggtaggtaa agggggtatt tgtggaaatg tataaCGatg gggttattgg gtggtaaata tttttttCGt tttttttgga gatgCGtaaa ttatagagaa taaatCGttt gatttaaggg tttgggggtg gaggagaggt

SIRPH Primer SN-3 (+1262 bp from the transcription start site)

ACIN1 (apoptotic chromatin condensation inducer 1) on NT_026437.11 (Genbank accession number) Wild type sequence 4526761 ctgggctgCG 4526821 cagggcactC 4526881 CGgggCGggt 4526941 gagCGCGCGg 4527001 CGgttccCGg 4527061 ttttgagagc 4527121 aagggtccag 4527181 tCGgCGggag 4527241 aacagagCGa 4527301 aggtgaccaa 4527361 tcctagccct 4527421 aaggtccaca 4527481 aataagcagg

gggCGcatac GggCGggagg gcaccCGgga tgaggtgggg ctggcttttg ccatgcaaag agagtcCGag gtCGgaggCG ggagctgcCG acccctaatt gctggggtgc gctttgggtt aagcagtcag

agatgCGgCG aCGtccaggc gCGCGaagct aattCGgagc ggactggtgt caCGgtagca gccCGggccc ggcagtggaa ccccctggtg taacCGtgca CGccaCGgac ggtcagggcc cctaaaggaa

cccctccCGg caCGgCGcca gccagtcccC ccactgCGga ggatggaatc ggtcagctgg agggagCGgg cccttcttgg gtagttgtgg caggaccagg acaggttgct aaggcagggg cacatggaat

acCGggccCG ggccatttcC GccCGcagCG gagaccCGgc ccagctgcaa gggacactta tCGggaacag gaagctCGgt gaggcaccaa aaccagaaga ccatgccttc ataaaagaCG gagagcaact

cagCGccCGg Gaagagctgg gaaaaCGggC tcCGggctcc ggCGgCGgCG aggaCGgcag aggcaCGaca tacaggggcc ggtggcaggc ggacccaccc cCGaatgagg tgggaagttc cctactccag

tttttttCGg taCGgCGtta gttagttttC ttattgCGga Bi-ACIN1-F2 4527001 CGgttttCGg ttggtttttg ggattggtgt ggatggaatt SN-1 4527061 ttttgagagt ttatgtaaag taCGgtagta ggttagttgg

atCGggttCG ggttattttC GttCGtagCG gagattCGgt

tagCGttCGg Gaagagttgg gaaaaCGggC ttCGggtttt

  Bisulfite converted sequence 4526761 ttgggttgCG 4526821 tagggtattC 4526881 CGgggCGggt 4526941 gagCGCGCGg

gggCGtatat GggCGggagg gtattCGgga tgaggtgggg

agatgCGgCG aCGtttaggt gCGCGaagtt aattCGgagt

ttagttgtaa ggCGgCGgCG gggatattta aggaCGgtag

4527121 aagggtttag agagttCGag gttCGggttt agggagCGgg tCGggaatag aggtaCGata SN-3 4527181 tCGgCGggag gtCGgaggCG ggtagtggaa ttttttttgg gaagttCGgt tataggggtt Bi-ACIN1-R2 4527241 aatagagCGa ggagttgtCG ttttttggtg gtagttgtgg gaggtattaa ggtggtaggt 4527301 aggtgattaa atttttaatt taatCGtgta taggattagg aattagaaga ggatttattt 4527361 ttttagtttt gttggggtgt CGttaCGgat ataggttgtt ttatgttttt tCGaatgagg 4527421 aaggtttata gttttgggtt ggttagggtt aaggtagggg ataaaagaCG tgggaagttt 4527481 aataagtagg aagtagttag tttaaaggaa tatatggaat gagagtaatt tttattttag

SIRPH Primer SN-1(-548 bp from the transcription start site) and SN-3(-415 bp from the transcription start site)              

BCL2 (antagonist of cell death) on NT_033903 (Genbank accession number) Wild type sequence 9342301 tcccaccttc 9342361 gtcctcaaat 9342421 tggcctctgc 9342481 accctcacct 9342541 tgccctgcCG 9342601 tctgtgccCG 9342661 aCGgagcccc 9342721 ctgccagcct 9342781 CGggcCGgcc 9342841 tctggggggc 9342901 cccaatgCGC 9342961 CGaccccttc

ctgactctag accagctgaa tgctcccctg caccttcCGg attcCGggcc gcaccaagaa CGctctgtcC ggctcagcCG acacagtagc CGCGgcaCGg Gtgctgggga CGtctctgtC

ctggtgtcca tgtctaactt tccttccatg tCGcagctct ctCGggagct cCGgccCGgg GggtcccCGc ctccctccCG tgctggaaag tttgcagagC cttggacctg Gctggatgtc

ctgagggaca ctgggtgtga ggtccactcc agctgcctcc tcCGCGagca ttataggggg atgaagcctc cccCGCGagg ggctgaccca GCGggatCGg ggcCGCGcag aggcagagcc

cactccaagt cattccagcc tccttgCGcC tccctgaagc gggcCGtggC CGccaataaa agtttccagC taccCGggCG aataggagCG gctgcCGgca cccaggccCG taagtttccc

cagttcccag cctagaagcc Gggggggatg cctctctgac Gcaatgcatc tctttctgga GacCGCGctg gCGctggccc agtgaccctc gCGctgCGgt CGagcCGcCG tCGtaagaca

Bisulfite converted sequence 9342301 ttttattttt ttgattttag ttggtgttta ttgagggata tattttaagt tagtttttag 9342361 gtttttaaat attagttgaa tgtttaattt ttgggtgtga tattttagtt tttagaagtt 9342421 tggtttttgt tgtttttttg tttttttatg ggtttatttt tttttgCGtC Gggggggatg Bi-BCL2-F1 9342481 atttttattt tatttttCGg tCGtagtttt agttgttttt tttttgaagt tttttttgat SN-1 9342541 tgttttgtCG atttCGggtt ttCGggagtt ttCGCGagta gggtCGtggC Gtaatgtatt 9342601 tttgtgttCG gtattaagaa tCGgttCGgg ttataggggg CGttaataaa tttttttgga 9342661 aCGgagtttt CGttttgttC GggttttCGt atgaagtttt 9342721 ttgttagttt ggtttagtCG ttttttttCG tttCGCGagg Bi-BCL2-R1 9342781 CGggtCGgtt atatagtagt tgttggaaag ggttgattta 9342841 tttggggggt CGCGgtaCGg tttgtagagC GCGggatCGg 9342901 tttaatgCGC Gtgttgggga tttggatttg ggtCGCGtag 9342961 CGattttttt CGtttttgtC Gttggatgtt aggtagagtt

SIRPH Primer SN-1 (-496 bp from the transcription start site).

agtttttagC GatCGCGttg tattCGggCG gCGttggttt aataggagCG gttgtCGgta tttaggttCG taagtttttt

agtgattttt gCGttgCGgt CGagtCGtCG tCGtaagata

CD44 on NT_009237 (Genbank accession number) Wild type sequence 33947641 CGcCGCGggg 33947701 ctCGgaggca 33947761 CGattattta 33947821 tgctacttct 33947881 cttCGctCGc 33947941 gccaacttcC 33948001 ccatcctCGt 33948061 tCGccCGCGc 33948121 gggactctgc 33948181 gcagcaagat 33948241 ggccctgggg 33948301 gggctgggat  

ccagCGggag caggcacccC cagcctcagc taaaacctct tccctccctc Gaggcagcct ccCGtcctcC cctcCGttCG ctCGtgcCGc gggtgCGggg gactggagtc ttgCGctaaa

aagaaagcca GCGacactcc agagcaCGgg gCGggctgct CGtcttaggt cattgcccag GcCGgcccct ctcCGgacac tgagcctggC tgctcagCGC aagtgagctg cCGttggaga

gtgCGtctct aggttcccCG gCGggggcag tagtcacagc cactgttttc CGgaccccag gcccCGCGcc catggacaag GcagatCGgt GgaccCGgCG tctgCGaagt atgtgtctgt

gggCGcaggg acccaCGtcc aggggccCGc cccccttgct aacctCGaat cctctgccag cagggatcct ttttggtggc gagtgccCGc gcagcccctc gcattgggct ggaagcacca

gccagtgggg ctggcagccc cCGggagggc tgggtgtgtc aaaaactgca gttCGgtcCG ccagctcctt aCGcagcctg CGcagcctgg CGgctgagtc cCGgaaagca tttggttgaa

Bisulfite converted sequence 33947641 CGtCGCGggg ttagCGggag aagaaagtta gtgCGttttt gggCGtaggg gttagtgggg 33947701 ttCGgaggta taggtatttC GCGatatttt aggtttttCG atttaCGttt ttggtagttt 33947761 CGattattta tagttttagt agagtaCGgg gCGggggtag aggggttCGt tCGggagggt Bi-CD44-F1 33947821 tgttattttt taaaattttt gCGggttgtt tagttatagt tttttttgtt tgggtgtgtt 33947881 tttCGttCGt tttttttttt CGttttaggt tattgttttt aatttCGaat aaaaattgta SN-2 33947941 gttaattttC Gaggtagttt tattgtttag CGgattttag tttttgttag gttCGgttCG 33948001 ttattttCGt ttCGtttttC GtCGgttttt gtttCGCGtt tagggatttt ttagtttttt Bi- CD44-R1 33948061 tCGttCGCGt ttttCGttCG tttCGgatat tatggataag ttttggtggt aCGtagtttg 33948121 gggattttgt ttCGtgtCGt tgagtttggC GtagatCGgt gagtgttCGt CGtagtttgg 33948181 gtagtaagat gggtgCGggg tgtttagCGC GgattCGgCG gtagtttttt CGgttgagtt 33948241 ggttttgggg gattggagtt aagtgagttg tttgCGaagt gtattgggtt tCGgaaagta 33948301 gggttgggat ttgCGttaaa tCGttggaga atgtgtttgt ggaagtatta tttggttgaa

SIRPH Primer SN-2 (+320 bp from the transcription start site)                

RARB (RETINOIC ACID RECEPTOR, β) on NT_022517 (Genbank accession number) Wild type sequence 25409521 agcacttctt 25409581 CGctgcctgc 25409641 gagaatcctg 25409701 gtagggttca 25409761 acagaagtag 25409821 ggaccagaat 25409881 CGagcagggt 25409941 tgcaagcatt 25410001 gattggcCGa 25410061 gctggcttgt 25410121 ccaaaaaagg 25410181 acctagagga

gtattgtttt ctctctggct ggagttggtg cCGaaagttc taggaagtga tcccccatgC ttgtctgggc tacttggaag gcaagcctgg ctgtcataat ggcagagttt taagcacttt

taaggtgaga gtctgctttt atgtcagact actCGcatat gctgttcaga Gagctgtttg acCGtCGggg gagaacttgg aaaatggtaa tcatgattCG gatggagttg tgcagacatt

Bisulfite converted sequence 25409521 agtatttttt 25409581 CGttgtttgt 25409641 gagaattttg 25409701 gtagggttta

gtattgtttt ttttttggtt ggagttggtg tCGaaagttt

taaggtgaga gtttgttttt atgttagatt attCGtatat

25409761 25409821 25409881 25409941 25410001 25410061 25410121 25410181

aataggaaag gcagggctgc agttgggtca attaggcaat ggcaggaggg aggactggga taggatcCGg gatctttctg atgatcattt gggctgggaa ggtggacttt cagtgcaagg

aaaaCGcCGg tgggagtttt tttgaaggtt tcaatctttc tctattcttt tgcCGagaaC aaCGcattCG ggaacccccC ggatcaatta aaagaccaac tctatgccat gagatcatgt

cttgtgCGct taagctctgt agcagccCGg attctgtgtg gccaaagggg GCGagCGatc gaaggctttt GcccCGgctg caggctttta agcctaCGtg ttgcctccac ttgactgtat

aataggaaag aaaaCGtCGg gtagggttgt tgggagtttt agttgggtta tttgaaggtt attaggtaat ttaatttttt Bi-RARBeta-F1 atagaagtag taggaagtga gttgtttaga ggtaggaggg tttatttttt SN-1 ggattagaat ttttttatgC Gagttgtttg aggattggga tgtCGagaaC SN-2 CGagtagggt ttgtttgggt atCGtCGggg taggattCGg aaCGtattCG tgtaagtatt tatttggaag gagaatttgg gatttttttg ggaatttttC Bi-RARBeta-R1 gattggtCGa gtaagtttgg aaaatggtaa atgattattt ggattaatta gttggtttgt ttgttataat ttatgattCG gggttgggaa aaagattaat ttaaaaaagg ggtagagttt gatggagttg ggtggatttt tttatgttat atttagagga taagtatttt tgtagatatt tagtgtaagg gagattatgt

tttgtgCGtt taagttttgt agtagttCGg attttgtgtg gttaaagggg GCGagCGatt gaaggttttt GtttCGgttg taggttttta agtttaCGtg ttgtttttat ttgattgtat

SIRPH Primer SN-1 (+90 bp from the transcription start site) and SN-2(+125 bp from the transcription start site)

TNFRSF10C ( tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain) on NT_023666 (Genbank accession number) Wild type sequence 1334521 agcctggaca 1334581 agttcctgtc 1334641 aggccatgcc 1334701 cttttccccC 1334761 gggaacctct 1334821 accagagatg 1334881 GcccCGgcCG 1334941 ccataccatg 1335001 gctgccagtg 1335061 ggggcaggga 1335121 gtcCGggcag 1335181 aggagggacc 1335241 aagggaggga 1335301 gcctcctgaa 1335361 taaccccatg 1335421 gaaaaaactt

ggaccCGgaa CGtccatgca ccttttctga GCGggtataa ccaCGCGcaC caaggggtga cctgatggcC gccCGgatcc agtccCGgcC gaCGgggaca gaCGaactCG CGgcCGCGag agttccagaa gacacagagC tttacattct ttcagCGctg

tcaaacCGca cccaccacca gtgcttggaa attcagaggC Gaactcagcc aggagCGctt Gaggcagggt ccaagaccct GCGgtccctg CGgcagggat cCGtCGgagt ggagcagaga tCGagagagg Ggttgcctct taaaCGtgtt tctttagcaa

ggccctgggt aaacccaggc gtgactgctg GctgCGctcC aaCGatttct cctacCGtta gCGacccagg aaagttCGtC gctggggaag gcctggccct caggggaaga ggCGgtcccc gagggagtca ctcattaatt ccttggagat ctttcacctc

cacCGctgcC cttcctggag caagtgacaa Gattctggca gatagatttt gggaactctg acccaggaCG GtCGtcatCG agCGcacctg ggtcacctgC actgggtccc ctggctgccc aggtggaacc aattaattag CGgtttaacc ctctgtcctg

Bisulfite converted sequence 1334881 GtttCGgtCG tttgatggtC Gaggtagggt gCGatttagg atttaggaCG 1334941 ttatattatg gttCGgattt ttaagatttt aaagttCGtC GtCGttatCG 1335001 gttgttagtg agtttCGgtC GCGgtttttg gttggggaag agCGtatttg Bi-TNFRSF10C-F1 1335061 ggggtaggga gaCGgggata CGgtagggat gtttggtttt ggttatttgC Sn-1 1335121 gttCGggtag gaCGaattCG tCGtCGgagt taggggaaga attgggtttt 1335181 aggagggatt CGgtCGCGag ggagtagaga ggCGgttttt ttggttgttt Bi-TNFRSF10C-R1 1335241 aagggaggga agttttagaa tCGagagagg gagggagtta aggtggaatt

Ggaaagagcc gtgctagggg gtgaccaCGc gtgcagctgt tgggagtttg gggacagagC gCGtCGggaa tCGCGgtcct gCGcCGggag GgcCGggcat CGggctgggc CGagccCGCG catagagtga ttaataaaat aacagccagt aaagtggcag

gCGtCGggaa tCGCGgtttt gCGtCGggag GgtCGggtat CGggttgggt CGagttCGCG tatagagtga

1335301 gttttttgaa gatatagagC Ggttgttttt tttattaatt aattaattag ttaataaaat 1335361 taattttatg tttatatttt taaaCGtgtt ttttggagat CGgtttaatt aatagttagt 1335421 gaaaaaattt tttagCGttg tttttagtaa tttttatttt ttttgttttg aaagtggtag

SIRPH Primer SN-1 (+400bp from the transcription start site).

Alu (accession numbers: Alu-J U14567; Alu-Sb U14568; Alu-Sb1 U14569; Alu-Sb2 U14570; Alu-Sc U14571; Alu-Sp U14572; Alu-Sq U14573; Alu-Sx U14574) Wild type sequences ps-alu ----------ggcCGggCGCGgtggctcaCGcctgtaatcccagcactttgggaggcCGa 50 sq-alu ----------ggcCGggCGCGgtggctcaCGcctgtaatcccagcactttgggaggcCGa 50 sx-alu ggCGggCGgaggcCGggCGCGgtggctcaCGcctgtaatcccagcactttggga------ 54 j-alu ----------ggcCGggCGCGgtggctcaCGcctgtaatcccagcactttgggaggcCGa 50 sb1-alu ----------ggcCGggCGCGgtggctcaCGcctgtaatcccagcactttgggaggcCGa 50 sb2-alu ----------ggcCGggCGCGgtggctcaCGcctgtaatcccagcactttgggaggcCGa 50 sb-alu ----------ggcCGggCGCGgtggctcaCGcctgtaatcccagcactttgggaggcCGa 50 sc-alu ----------ggcCGggCGCGgtggctcaCGcctgtaatcccagcactttgggaggcCGa 50 sp-alu sq-alu sx-alu j-alu sb1-alu sb2-alu sb-alu sc-alu

ggCGggCGgatcacctgaggtCGggagttCGagaccagcctgaccaacatggagaaaccc ggCGggtggatcacctgaggtcaggagttCGagaccagcctggccaacatggtgaaaccc ----ggaagatcacctgaggtcaggagttCGagaccagcctggccaacatggtgaaaccc ggCGggaggatcacttgagcccaggagttCGagaccagcctgggcaacatagtgaaaccc ggCGggCGgatca--CGaggtcaggagatCGagaccatccCGgctaaaaCGgtgaaaccc ggCGggtggatca--tgaggtcaggagatCGagaccatcctggctaacaaggtgaaaccc ggCGggCGgatca--CGaggtcaggagatCGagaccatcctggctaacaCGgtgaaaccc ggCGggCGgatca--CGaggtcaagagatCGagaccatcctggccaacatggtgaaaccc

110 110 110 110 108 108 108 108

sp-alu sq-alu sx-alu j-alu sb1-alu sb2-alu sb-alu sc-alu

CGtctctactaaaaatacaaaaa-ttagcCGggCGtggtggCGcatgcctgtaatcccag CGtctctactaaaaatacaaaaa-ttagcCGggCGtggtggCGggCGcctgtaatcccag CGtctctactaaaaatacaaaaa-ttagcCGggCGtggtggCGCGCGcctgtaatcccag CGtctctacaaaaaatacaaaaa-ttagcCGggCGtggtggCGCGCGcctgtagtcccag CGtctctactaaaaatacaaaaa-ttagcCGggCGtagtggCGggCGcctgtagtcccag CGtctctactaaaaatacaaaaaattagcCGggCGCGgtggCGggCGcctgtagtcccag CGtctctactaaaaatacaaaaa-ttagcCGggCGtggtggCGggCGcctgtagtcccag CGtctctactaaaaatacaaaaa-ttagctgggCGtggtggCGCGCGcctgtagtcccag

169 169 169 169 167 168 167 167

sp-alu sq-alu sx-alu j-alu sb1-alu sb2-alu sb-alu sc-alu

ctactCGggaggctgaggcaggagaatCGcttgaaccCGggaggCGgaggttgCGgtgag ctactCGggaggctgaggcaggagaatCGcttgaaccCGggaggCGgaggttgcagtgag ctactCGggaggctgaggcaggagaatCGcttgaaccCGggaggCGgaggttgcagtgag ctactCGggaggctgaggcaggaggatCGcttgagccCGggaggtCGaggctgcagtgag ctacttgggaggctgaggcaggagaatggCGtgaaccCGggaggCGgagcttgcagtgag ctactggggaggctgaggcaggagaatggCGtgaaccCGggaagCGgagcttgcagtgag ctactCGggaggctgaggcaggagaatggCGtgaaccCGggaggCGgagcttgcagtgag ctactCGggaggctgaggcaggagaatCGcttgaaccCGggaggCGgaggttgcagtgag

229 229 229 229 227 228 227 227

sp-alu sq-alu sx-alu j-alu sb1-alu sb2-alu sb-alu sc-alu

cCGagatCGCGccattgcactcca-------gcctgggcaacaagagCGaaactcCGtct cCGagatCGCGccactgcactcca-------gcctgggcaacaagagCGaaactcCGtct cCGagatCGCGccactgcactcca-------gcctgggCGaca-gagCGagactcCGtct cCGtgatCGCGccactgcactcca-------gcctgggCGaca-gagCGagaccctgtct cCGagatccCGccactgcactcca-------gcctgggCGaca-gagCGagactcCGtct cCGagattgCGccactgcagtcCGcagtcCGgcctgggCGaca-gagCGagactcCGtct cCGagatCGCGccactgcactcca-------gcctgggCGaca-gagCGagactcCGtct cCGagatCGCGccactgcactcca-------gcctgg-CGaca-gagCGagactcCGtct

282 282 281 281 279 287 279 278

Sp-Alu Sq-Alu Sx-Alu J-Alu Sb1-Alu Sb2-Alu Sb-Alu Sc-Alu  

CAAAAAAAA CAAAAAAAA CAAAAAAAA CAAAAAAAA CAAAAAAAA CAAAAAAAA CAAAAAAAA CAAAAAAAA

291 291 290 290 288 296 288 287

Bisulfite sequences Sp-Alu sq-alu sx-alu j-alu sb1-alu sb2-alu

Bi-Alu-All-F3 ----------ggtCGggCGtggtggtttaCGtttgtaattttagtattttgggaggtCGa ----------ggtCGggCGtggtggtttaCGtttgtaattttagtattttgggaggtCGa ggCGggCGgaggtCGggCGtggtggtttaCGtttgtaattttagtattttggga---------------ggtCGggCGtggtggtttaCGtttgtaattttagtattttgggaggtCGa ----------ggtCGggCGtggtggtttaCGtttgtaattttagtattttgggaggtCGa ----------ggtCGggCGtggtggtttaCGtttgtaattttagtattttgggaggtCGa

50 50 54 50 50 50

sb-alu sc-alu

----------ggtCGggCGtggtggtttaCGtttgtaattttagtattttgggaggtCGa 50 ----------ggtCGggCGtggtggtttaCGtttgtaattttagtattttgggaggtCGa 50

sp-alu sq-alu sx-alu j-alu sb1-alu sb2-alu sb-alu sc-alu

ggCGggCGgattatttgaggtCGggagttCGagattagtttgattaatatggagaaattt ggCGggtggattatttgaggttaggagttCGagattagtttggttaatatggtgaaattt ----ggaagattatttgaggttaggagttCGagattagtttggttaatatggtgaaattt ggCGggaggattatttgagtttaggagttCGagattagtttgggtaatatagtgaaattt ggCGggCGgatta--CGaggttaggagatCGagattatttCGgttaaaaCGgtgaaattt ggCGggtggatta--tgaggttaggagatCGagattattttggttaataaggtgaaattt ggCGggCGgatta--CGaggttaggagatCGagattattttggttaataCGgtgaaattt ggCGggCGgatta--CGaggttaagagatCGagattattttggttaatatggtgaaattt

110 110 110 110 108 108 108 108

sp-alu sq-alu sx-alu j-alu sb1-alu sb2-alu sb-alu sc-alu

SN-1 SN-3 CGtttttattaaaaatataaaaa-ttagtCGggCGtggtggCGtatgtttgtaattttag CGtttttattaaaaatataaaaa-ttagtCGggCGtggtggCGggCGtttgtaattttag CGtttttattaaaaatataaaaa-ttagtCGggCGtggtggCGCGCGtttgtaattttag CGtttttataaaaaatataaaaa-ttagtCGggCGtggtggCGCGCGtttgtagttttag CGtttttattaaaaatataaaaa-ttagtCGggCGtagtggCGggCGtttgtagttttag CGtttttattaaaaatataaaaaattagtCGggCGCGgtggtgggCGtttgtagttttag CGtttttattaaaaatataaaaa-ttagtCGggCGtggtggCGggCGtttgtagttttag CGtttttattaaaaatataaaaa-ttagttgggCGtggtggCGCGCGtttgtagttttag

169 169 169 169 167 168 167 167

sp-alu sq-alu sx-alu j-alu sb1-alu sb2-alu sb-alu sc-alu

ttattCGggaggttgaggtaggagaatCGtttgaattCGggaggCGgaggttgCGgtgag ttattCGggaggttgaggtaggagaatCGtttgaattCGggaggCGgaggttgtagtgag ttattCGggaggttgaggtaggagaatCGtttgaattCGggaggCGgaggttgtagtgag ttattCGggaggttgaggtaggaggatCGtttgagttCGggaggtCGaggttgtagtgag ttatttgggaggttgaggtaggagaatggCGtgaattCGggaggCGgagtttgtagtgag ttattggggaggttgaggtaggagaatggCGtgaattCGggaagCGgagtttgtagtgag ttattCGggaggttgaggtaggagaatggCGtgaattCGggaggCGgagtttgtagtgag ttattCGggaggttgaggtaggagaatCGtttgaattCGggaggCGgaggttgtagtgag Bi-Alu-All-R2 tCGagatCGCGttattgtatttta-------gtttgggtaataagagtgaaatttCGttt tCGagatCGCGttattgtatttta-------gtttgggtaataagagtgaaatttCGttt tCGagatCGCGttattgtatttta-------gtttgggCGata-gagCGagatttCGttt tCGtgatCGCGttattgtatttta-------gtttgggCGata-gagCGagattttgttt tCGagatttCGttattgtatttta-------gtttgggCGata-gagCGagatttCGttt ttgagattgCGttattgtagttCGtagttCGgtttgggCGata-gagCGagatttCGttt tCGagatCGCGttattgtatttta-------gtttgggCGata-gagCGagatttCGttt tCGagatCGCGttattgtatttta-------gtttgg-CGata-gagCGagatttCGttt

Sp-Alu Sq-Alu Sx-Alu J-Alu Sb1-Alu Sb2-Alu Sb-Alu Sc-Alu

TAAAAAAAA TAAAAAAAA TAAAAAAAA TAAAAAAAA TAAAAAAAA TAAAAAAAA TAAAAAAAA TAAAAAAAA

sp-alu sq-alu sx-alu j-alu sb1-alu sb2-alu sb-alu sc-alu

291 291 290 290 288 296 288 287

SIRPH Primer SN-1, SN-3 and SN-4  

229 229 229 229 227 228 227 227 282 282 281 281 279 287 279 278

LINE-1 elements (accession number X52235) Wild type sequence 61 121 181 241 301 361 421 481

CGcagaagac gtgccagaca Gaggcattgt aaggggtgaC acCGgcttaa CGcccaCGga gcaaggctgg tggcagctCG

gggtgatttc gtgggCGcag ctcacttggg GgaCGcacct aaaaCGgCGc gtctCGctga gggaggggCG aactgggtgg

tgcatttcca gtcagtgggt aagCGcaagg ggaaaatCGg acCGCGagat ttgctagcac ccCGccattg agcccaccac

tctgaggtac gCGCGcacCG ggtcagggag gtctctccca tatatcttgc agcagtctga cccaggcttg agctcaagga

tgggttcatc tcactaggga tgcaCGagcC GaagcagggC ttccctttcC Gagtcaaaga ccCGaatatt9 gCGctttCGg acctggctag gagggtccta gatcaaactg caaggcCGca cttgggtaaa caaagcagcc ggcctgcctg cctttgtagg

61 CGtagaagat gggtgatttt tgtattttta 121 gtgttagata gtgggCGtag gttagtgggt 181 Gaggtattgt tttatttggg aagCGtaagg SN-1 241 aaggggtgaC GgaCGtattt ggaaaatCGg

tttgaggtat gCGCGtatCG ggttagggag SN-8 gtttttttta

301 atCGgtttaa aaaaCGgCGt atCGCGagat 361 CGtttaCGga gtttCGttga ttgttagtat 421 gtaaggttgg gggaggggCG ttCGttattg Bi-LINE-1-R1 481 tggtagttCG aattgggtgg agtttattat

tatattttgt atttggttag gagggtttta agtagtttga gattaaattg taaggtCGta tttaggtttg tttgggtaaa taaagtagtt

Bisulfite sequence

SIRPH Primer: SN-1 and SN-8.

 

Bi-LINE-1-F1 tgggtttatt ttattaggga tgtaCGagtC GaagtagggC tttttttttC Gagttaaaga ttCGaatatt gCGttttCGg

agtttaagga ggtttgtttg tttttgtagg