fluorescence images were merged into single images. (h) ITs can be seen inside the curled and GUS- stained root hairs. Scale bars are 1 mm (a-f) and 100 µm ...
Figure S1
a AtEXPA7_aa AtEXPA18_aa LjEXPA7_chr4.CM0126.580.r2.m_aa MtEXPA7_aa LjEXPA8_chr2.CM0021.2840.r2.m_aa MtEXPA8_aa
1 1 1 1 1 1
G G A CG Y G N L F NS GY G L S T A A LS T T L F N D G YG C G Q C F Q IT C SK S PH C Y SG K G G A CG Y G N M Y DS GY G V A T T A LS T AL F N E G YA C G Q C F Q L KC VS S PN C Y YG S G G A CG Y G N L F LN GY G T D T A A LS S T L F N N G YA C G T C Y Q I KC V Q AS A C Y GN V G G A CG Y G N L F VN GY G T D T A A LS S T L F N N G YA C G T C Y Q I KC V Q S S A C N TN V G G A CG Y G N L F QS GY G T D T V A LS S T L F N N G YA C G T C Y Q I KC Y Q S S A C Y KN V G G A CG Y G N L F QN GY G T D T V A LS S T L F N N G YA C G T C F Q I KC Y Q S S A C Y RN V
S- T VV T A T N L C P PN W Y QD S NA G G W C N P P R TH F D M AK P A FM K L A Y W R A GI I PA T VV T A T N I C P PN Y G QA S N NG G W C N P P R VH F D L TK P A FM K I A N W K A GI I PY T TV T A T N L C P PN W S EA S D NG G W C N P P R TH F D M AK P A FM K I A Q W K A GI V PY T TV T A T N I C P PN W S QA S D NG G W C N P P R SH F D M PK P A FM K I A Q W K A GI R AF T TV T A T N L C P PN W S KP S D NG G W C N P P R AH F D M SK P A FM K I A Q W K A GI V AF T TV T A T N L C P PN W S KP S D NG G W C N P P R VH F D M AK P A FM K I A Q W K A GI V
P V A YR R V P C Q R SGG M R F Q F Q GN S Y W L L I P V S YR R V A C K K IGG I R F K F E GN G Y W L L V P V M YR R V P C V R RGG L R F S F Q GN G Y W L L V P V M YR T V P C V R SE G F R F S F Q GN G Y W L L V P V L FR R V P C Q S KGG V R F S F Q GN G Y W L L V P V M YR R V P C Q R KGG I R F S F Q GN G Y W L L V
Figure S1 The multiple alignment of expansin protein sequences by CLUSTAL W. (a) The amino acid sequence between 55 (as “1” in the illustration) and 181 (as “127” in the illustration) of AtEXPA7 and the equivalent regions of each expansin protein sequence were aligned by CLUSTAL W. Residues that are identical among all amino acid sequences (black) and conserved substitutions (gray) are indicated. (b) Phylogenetic tree based on the amino acid sequence between 55 (Gly) and 181 (Ile) of AtEXPA7 and the equivalent regions of each expansin protein sequence.
Figure S2
RH101
0.4 0.3 0.2 0.1 0
0.5
Whole roots Root hairs Stripped roots
b
b a
a
Control
a
a
+M. loti
Relative transcript level
Relative transcript level
0.5
RH102
Whole roots Root hairs Stripped roots
0.4
b
0.3
b
0.2 0.1 0
a
a
Control
a
a
+M. loti
Figure S2 Root hair-specific expression analysis of RH101 and RH102 in response to infection by M. loti. Real-time RT-PCR analysis of RH101 and RH102 transcripts in root hair cells, stripped roots or whole roots 2 days after mock-inoculation (Control) or inoculation with M. loti (+M. loti). The data are indicated as means and SD of 3 or 4 biological replications. Means with different letters are significantly different (Tukey-Kramer multiple comparison test, P
