Figure S2 ABCD - PLOS

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Figure S2. MI score > 3.0. HK. RR. 13. 15. 16. 250. 251. 254. 255. 257. 258. 261. 269. 272. 273 ... 88. HK853. RR468. 230. 240. 250. 260. 270. 280. 290. EnvZ AGVKO. EnvZ ... 300. HK. EnvZ. RstB. CpxA. HK853. 310. 320. 330. 340. 350. 360.
Figure S2

A

D

MI score > 3.0 HK

RR

250 251 254 255 257 258 261 269 272 273

13 15 16 19 22 23 108 110

234 235 246 247 350 351 392 393 394

B

HK

C

57 84 85 88 91

HK853

RR468

EnvZ RstB CpxA HK853

230 240 250 260 270 280 290 AGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQ--------DGYLAESINKDIEECNAIIEQFIDYLRTG---QEM DNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSDNL--------SAAESQALNRDISQLEALIEELLTYARLDRPQNEL TALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRR------SGESKELERIETEAQRLDSMINDLLVMSRNQQ-KNAL ERLKRIDRMKTEFIANISHELRTPLTAIKAYAETIYNSLGELDLSTLKEFLEVIIDQSNHLENLLNELLDFSRLERKSLQI

EnvZ RstB CpxA HK853

300 310 320 330 340 350 360 PMEMADLNAVLG----EVIAAESGYEREIETALYP-GSIEVKMHPLSIKRAVANMVVNAARYGNGW-----IKVSSGTEPN HLSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQG-HYAALDMR--LMERVLDNLLNNALRYCHST-----VETSLLLSGN VSETIKANQLWSEVLDNAAFEAEQMGKSLTVNFPP-GPWPLYGNPNALESALENIVRNALRYSHTK-----IEVGFAVDKD NREKVDLCDLVESAVNAIKEFASSHNVNVLFESNVPCPVEAYIDPTRIRQVLLNLLNNGVKYSKKDAPDKYVKVILDEKDG

EnvZ RstB CpxA HK853

370 380 390 400 410 420 430 440 450 RAWFQVEDDGPGIAPEQRKHLFQPFVRGDSAR--TISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG RATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLAIVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA GITITVDDDGPGVSPEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIWLPLYKRS GVLIIVEDNGIGIPDHAKDRIFEQFYRVDSSLTYEVPGTGLGLAITKEIVELHGGRIWVESEVGKGSRFFVWIPKDRAGEDNRQDN

α1

β1

α2

β2

α3

β5

β3

β4

α4

α5

ATP lid

β6

β7

OmpR RstA CpxR RR468

10 20 30 40 50 60 70 MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLLTRESFHLMVLDLMLPGEDGLSICRRL ---MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDGMTICRDL ---MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDD-SIDLLLLDVMMPKKNGIDTLKAL --MSKKVLLVDDSAVLRKIVSFNLKKEGYEVIEAENGQIALEKLSEFTPDLIVLDIMMPVMDGFTVLKKL

OmpR RstA CpxR RR468

80 90 100 110 120 RSQS--NPMPIIMVTAKGEEVDRIVGLEIGADDYIPKPFNPRELLARIRAVLRRQAN RAKW---SGPIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNEQ RQTH---QTPVIMLTARGSELDRVLGLELGADDYLPKPFNDRELVARIRAILRRSHW QEKEEWKRIPVIVLTAKGGEEDESLALSLGARKVMRKPFSPSQFIEEVKHLLNE

RR

α1

β1

β4

α2

β2

α4

β5

β3

α5

α3

= highly conserved residues

}

= covarying residues