Figure S2 - PLOS

CC15 CC17 CC14 CC13 CC10 CC4 CC2 CC1

SR15

SR24 SR25 SR28 SR4 SR1 SR12 SR14 SR23 SR30 SR21 SR2 SR3 SR8 SR11 SR5 SR10 SR22 SR26 SR13 SR17 SR18 SR29 E7 MI7 MI8 CC5 CC6 CC7 CC8 CC9 CC11 CC12 CC16 SR19 SR16 SR20 SL1 SL2 MI1 MI4 MI2 MI3 MI5 SL5 LK07-02 LK07-03 LK07-01 SR6 LK07-05 SR7 SR9 NNKB01 BP05 BP06 BP14 NNKB02 NNKB03 BP02 BP08 BP09 BP12 BP13 BP15 NNKB05 BP16 NNKB06 NNKB09 NNKB10 NNKB08 SL6 BP01 BP04 BP07 BP10 BP11 NNKB04 SL4

Locations: CC - Charlotte Co., FL SR - Shark & Harney River, FL EP - Everglades NP, FL MI - Marco Island, FL SL - St. Lucie Co., FL LK - Long Key, FL NNK - No Name Key, FL BP - Big Pine Key, FL EI - Exuma Island, Bahamas SSI - San Salvador Island, Bahamas PG - Papa Gabriel, Twin Cays, Belize Dan - Dangriga, Belize Hon - Honduras PanRS - Panama Gitmo - Cuba Koce - K. ocellatus, Guaratiba, Brazil Hy - Nagoya Higashiyama Zoo (obtained by Zoo from a German fish hobbyist, locality unknown)

BP03 NNK5 PG91-7 NNK4 NNK2 NNKB07 EI4 EI6 EI3 EI2 EI1 EI5

E3 PG01 PG06 PG02 PG03 PG05 PG07

SSI1 = 02RhlA SSI2 SSI3 SSI4 SSI5 LK07-04 LK07-06

PG04 PG08 PG91-2 PG91-4 PG33 PG48 PG36 PG44 NNK1 NNK3 PG09 PG35 PG38 PG39 PG40 PG41 PG43 PG46 PG47 PG34 PG45 HonR2 Hon2 Hon11 Hon7 Hon9

K2P 0.005 K. marmoratus

Dan2k PG10 PG37 PG42

K. ocellatus

Figure S2

PanRS Gitmo Koce204 Koce205 Koce206 Koce201 = 03HyA Koce202 Koce203 Koce207 Koce208 Koce209 Koce210