... EEY DSSYD- I DVD ELV I SMRTDL NRFMSQAEDR EKGNRCCLIC KIVHR I ... IVKIT YQFFA HD - AETILLL TKN ISGFMEG TIYHFPKV IN EANRPEDEYH ...
MA T - - - - E E Y MA Y V K T GQ E Y ME T - - - - E K Y MV T - - - - E E Y MA R - - - - E E Y M T Y V K T GQ E Y MV T - - - - E E Y MF T - - - - EQY
NSSYD - VDLD DS SCD - VD I D DN L DD - VD I D DSSYE - VD I D DS SCD - VD I D DSSYD - VD I D DSSYD - I DVD DS T HDDVD I D
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GNGN R C C L E NGN R C C L K NGN R C C L E KGN R C C L QK V N R C C L V KGK R C C L E KGN R C C L KNRNNYGL
SNAY E P S V L S SNAY E P S V L S RNAY E PGV I S SNAY E P S V L S NNAYKP S V L S SNAY E P SA L S I NAY E P S V L S RNAY E P S V L S
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L QAMEME KW I L QAME T E KW I L L AME T E KW I L V AME T E KW I L QAME T E KW I L QAME T E KW I L L AME T E KW I L L AMEME KW I
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NMD S NMD S Y I DS NMD S YMD S NMG S NMD S DMD S
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T NR T NK TNS T NR T KN T NN TNS T SD
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YRF F S YQF - S YQF I S YRF F S YQF F A YQF S S YQL F S YQL F S
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S E F ME G S E F ME G S E F ME G S E F ME G S G F ME G C E F ME G S E F ME G S E F ME G
L YH F PKA L YHL PKV L YHF PKV L YHF PK I I YHF PKV L YHF PKV L YHF PKV L YHF PKV
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VHR VHR VHR VHR VHR VHR VHR I YR
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RH T D RQ T D RQA D RL TD RQ T D RQ T D RQ T D RL TD
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PFF PFF PFF PFF PFF PFF PFF PFF
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EDNRP ED F YH EANRP ED F YH EANRPDE F YH EANRP ED F YH EANRP ED F YH EANRP ED F YH EANRP ED F YH EANRPRD F SH
L L H L CHKY F K L L H L CHKY L K L L H L CHK F L K L L H L CHKY L K L L H L CHKY L K L L H L CHKY L K L L H L CHKY L K L L H L C HMY L K
P S H N L E DGH H P SHK L EDDNH P SHD L DDDRH P SHK I EDDHD P SHK L ECDHH P S H N L E D DQH P SHK L DDDHH P S H K L E D E QH
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C L P I DDKS S L C L P I DDKS S L C L P I DDKS S L C L P I DDKS S L C L P I DDKS S L - - - - - - - - - V CQ * - - - - - C L P I DDKS S L
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SDEDVS T L F T SDEDVS T L F T SDEDVS T L F T SDEDVXXXXX SDEDVS T L F T - - - - - - - - - - - - - - - - - - SDEEVS T L F T
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H N H F S N PWV G H N H F S N PWV G H N H F S N PWV G H N H F S N PWV G H N H F S N PWV G - - - - - - - - - - - - - - - - - - H K H F S N PWL G
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F AA F AA F AA F AA F AA - - - - F AA
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Figure S2. Protein sequence alignment of LpSDUF247 orthologs from self-incompatible and self-compatible Poaceae species The alignment includes self-incompatible (SI) perennial ryegrass (Lolium perenne L.), annual ryegrass (Lolium multiflorum Lam.), westerwolds ryegrass (Lolium multiflorum Lam. var. westerwoldicum Wittm.) and meadow fescue (Festuca pratensis Huds.) as well as self-compatible (SC) darnel (Lolium temulentum L.), oat (Avena atlantica B. R. Baum & G. Fedak), wild rice (Oryza brachyantha) and Tausch's goatgrass (Aegilops tauschii). Stop codons are represented by an asterisk, X indicates unknown protein sequence.