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time (s). 0. 400. 800. RU. 800. 400. 0. 0. 40. 80. 120 time (s). RU. 0 ÂµM. 500 ÂµM. 0 ÂµM. 500 ÂµM. Req. 0 1 2 3 4 5. 250. 500. 750. KD=67Â±6Î¼M. 0 1 2 3 4 5.

A

RU

800

Req 750 500

500 µM

KD=67±6μM

250

0 1 2 3 4 5 [PriL-CTD] (102 · μM)

400 0 µM

0

0

B

RU

800

80

40 time (s)

120

Req 750

500 µM

500 250

KD=168±18μM

0 1 2 3 4 5 [PriL-CTD] (102 · μM)

400 0 µM

0

0

40

time (s)

Figure S3

80

120